Abstract
Thewaxy proteins encoded by the genomes A, B, and D in polyploid wheats and related diploid species were isolated by SDS-PAGE. The N-terminal amino acid sequences of mature proteins and V8 protease-induced fragments were determined. A total of five amino acid substitutions was detected in these sequences, which represent about 10% of the whole sequences of thewaxy proteins. A comparison of these sequences in polyploid wheats with those in related diploid species revealed the following: (i)waxy proteins encoded by the A genome of polyploid wheats were identical to that ofTriticum monococcum, (ii) thewaxy protein encoded by the B genome ofT. turgidum was identical to that ofT. searsii, but differed from those ofT. speltoides andT. longissimum by one amino acid substitution, (iii) thewaxy protein encoded by the B genome ofT. aestivum differed from that encoded by the B genome ofT. turgidum by one amino acid substitution, and (iv) thewaxy protein encoded by the D genome ofT. aestivum was identical to that ofT. tauschii.
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References
Ainsworth, C. C., Clark, J. R., and Balsdon, J. (1993). Expression, organisation and structure of the genes encoding thewaxy protein (granule-bound starch synthase) in wheat.Plant Mol. Biol. 2267.
Chao, S., Sharp, P. J., Worland, A. J., Warham, E. J., Kocbner, R. M. D., and Gale, M. D. (1989). RFLP-based genetic maps of wheat homoeologous group 7 chromosomes.Theor. Appl. Genet. 78495.
Clark, J. R., Robertson, M., and Ainsworth, C. C. (1991). Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding thewaxy protein.Plant Mol. Biol. 161099.
Kimber, G. (1974). A reassessment of the origin of the polyploid wheats.Genetics 78487.
Konarev, V. G. (1983). The nature and origin of wheat genomes on the data of grain protein immunochemistry and electrophoresis.Proceedings of the 6th International Wheat Genetics Symposium, Kyoto, Japan.
Konarev, V. G., Gavrilyuk, I. P., Gubareva, N. K., and Peneva, T. I. (1979). Seed proteins in genome analysis, cultivar identification and documentation of cereal genetic resources: A review.Cereal Chem. 56272.
Kyhse-Anderson, J. (1984). Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.J. Biochem. Biophys. Methods 10203.
Nishikawa, K., Furuta, Y., Yamada, T., and Kudo, S. (1992). Genetic studies of α-amylase isozymes in wheat. VII. Variation in diploid ancestral species and phylogeny of tetraploid wheat.Jpn. J. Genet. 671.
Taira, T., Uematsu, M., Nakano, Y., and Morikawa, T. (1991). Molecular identification and comparison of the starch synthase bound to starch granules between endosperm and leaf blades in rice plants.Biochem. Genet. 29301.
Taira, T., Fujita, N., Takaoka, K., Uematsu, M., Wadano, A., Kozaki, S., and Okabe, S. (1995). Variation in primary structure ofwaxy proteins (granule-bound starch synthase) in diploid cereals.Biochem. Genet. 33269.
Takumi, S., Nasuda, S., Lui, Y.-G., and Tsunewaki, K. (1993). Wheat phylogeny determined by RFLP analysis of nuclear DNA. 1. Einkorn wheat.Jpn. J. Genet. 6873.
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Fujita, N., Wadano, A., Kozaki, S. et al. Comparison of the primary structure ofwaxy proteins (granule-bound starch synthase) between polyploid wheats and related diploid species. Biochem Genet 34, 403–413 (1996). https://doi.org/10.1007/BF00570121
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DOI: https://doi.org/10.1007/BF00570121