Skip to main content
Log in

Alterations in protein metabolism in selected tissues of fish, Cyprinus carpio, during sublethal concentration of cypermethrin

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Freshwater fish, Cyprinus carpio, were exposed to sublethal concentration of cypermethrin (20 µg/l) for 6, 12, 24 and 48 h to determine the protein fractions, amino acids, protease, alanine aminotransferase, aspartate aminotransferase, ammonia, urea and glutamine levels in gill, brain, liver and muscle tissues. Total, structural and soluble proteins showed decrement; whereas free amino acids and the activities of protease, aspartate aminotransferase and alanine aminotransferase significantly increased at all exposure periods in cypermethrin-exposed fish. Interestingly, ammonia content decreased but urea and glutamine levels increased in all the tissues during cypermethrin stress. It was observed that the changes steadily increased with an increase in the period of exposure and exhibited tissue specificity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Armes, N. J. A. R., Jadhav, D. R., Bond, G. S. and King, A. B. S.: 1992, ‘Insecticide resistance in Helicoverpa armigera in South India’, Pestic. Sci. 34, 355–364.

    Google Scholar 

  • Bradbury, S. P., Mckim, J. R. and Coats, J. R.: 1987, ‘Physiological responses of rainbow trout, Salmo gairdneri to acute fenvalerate intoxication’, Pestic. Biochem. Physiol. 27, 275–285.

    Google Scholar 

  • Bradbury, S. P. and Coats, J. R.: 1989, ‘Comparative toxicology of the pyrethroid insecticides’, Rev. Environ. Contam. Toxicol. 108, 143–177.

    Google Scholar 

  • Bergmeyer, H. U.: 1965, in: Methods of Enzymatic Analysis. Academic Press, New York.

    Google Scholar 

  • Casida, J. E.: 1980, ‘Pyrethrum flowers and pyrethroid insecticides’, Environ. Hlth. Perspect. 34, 189–202.

    Google Scholar 

  • Casida, J. E., Gammon, D. W., Glickman, A. H. and Lawrence, L. R.: 1983, ‘Mechanisms of selective action of pyrethroid insecticides’, Ann. Rev. Pharmacol. Toxicol. 23, 413–438.

    Google Scholar 

  • Colowick, S. P. and Kaplon, N. O.: 1967, in: Methods in Enzymology. Academic Press, New York.

    Google Scholar 

  • Lowenstein, J. M. and Goodman, M. N.: 1978, ‘The purine nucleotide cycle in skeletal muscle’, Fedn Proc. Fedn Am. Socs. exp. Biol. 37, 2308–2312.

    Google Scholar 

  • Lowry, O. H., Rosenbrough, N. J., Farr, A. R. and Randall, R. J.: 1951, ‘Protein measurement with folin phenol reagent’, J. Biol. Chem. 193, 165–173.

    Google Scholar 

  • Malla Reddy, P. and Bashamohideen, Md.: 1989, ‘Toxicity of synthetic pyrethroid insecticides, fenvalerate and cypermethrin to the fish, Cyprinus carpio’, Environ. Ecol. 7, 1016–1018.

    Google Scholar 

  • Malla Reddy, P. and Bashamohideen, Md.: 1995, ‘Modulations in the levels of repiration and ions in the fish, Cyprinus carpio, exposed to cypermethrin’, Environ. Monit. Assess. (in Press).

  • Malla Reddy, P. and Harold Philip, G.: 1994, ‘In vivo inhibition of AChE, ATPase activities in the tissues of freshwater fish, Cyprinus carpio, exposed to technical grade cypermethrin, Bull. Environ. Contam. Toxicol. 52, 619–626.

    Google Scholar 

  • McCaffery, A. R., King, A. B. S., Walker, A. J. and El-Nayir, H.: 1989, ‘Resistance to synthetic pyrethroids in the bull worm, Heliothis armigera from Andhra Pradesh, India’, Pestic. Sci. 27, 65–76.

    Google Scholar 

  • Miyamoto, J.: 1976, ‘Degradation, metabolism and toxicity of synthetic pyrethroids’, Environ. Hlth. Perspect. 14, 15–28.

    Google Scholar 

  • Mommsen, T. P. and Walsh, P. J.: 1992, ‘Biochemical and environmental perspectives on nitrogen metabolism in fishes’, Experientia 48, 583–593.

    Google Scholar 

  • Moore, S. and Stein, W. H.: 1954, ‘A modified ninhydrin reagent for the photometric determination of amino acids and related compounds’, J. Biol. Chem. 211, 907–913.

    Google Scholar 

  • Moorthy, K. S., Kasi Reddy, B., Swami, K. S. and Chetty, C. S.: 1984, ‘Changes in respiration and ionic content in the tissues of freshwater mussel exposed to methyl parathion toxicity’, Toxicol. Lett. 21, 287–291.

    Google Scholar 

  • Natarajan, G. M.: 1985, ‘Inhibition of branchial enzymes in snake head fish (Channa striatus) by oxydemeton-methyl’, Pestic. Biochem. Physiol. 23, 41–46.

    Google Scholar 

  • Netelson, S.: 1971, in: Techniques of Clinical Chemistry, Charles, (Ed.), Thomas Publishers, Spring-field, Illinois.

    Google Scholar 

  • Pillai, S. K. and Sinha, H. C.: 1968, in: Statistical Methods for Biological Workers, Ramprasad & Sons, Agra, India.

    Google Scholar 

  • Ravinder, V., Suryanarayana, N. and Narayana, N.: 1988, ‘Decis induced biochemical alterations in a freshwater fish, Clarias batrachus’, Ind. J. Comp. Anim. Physiol. 6, 5–12.

    Google Scholar 

  • Reitman, S. and Frankel, S.: 1957, ‘A colorimetric method for the determination of glutamic oxaloacetate and glutamic pyruvic transaminases’, Amer. J. Clinc. Pathol. 289, 56–63.

    Google Scholar 

  • Sambasiva Rao, K. R. S.: 1987, ‘Variations in the nitrogen products of Channa punctatus augumented by interaction of carbaryl and phenthoate’, J. Environ. Biol. 8, 173–177.

    Google Scholar 

  • Seshagiri Rao, K., Srinivasa Moorthy, K., Kasi Reddy, B., Swami, K. S. and Chetty, C. S.: 1987, ‘Effect of benthiocarb on protein metabolism of teleost, Sarotherodon mossambicus’, Ind. J. Environ. Hlth. 29, 45–50.

    Google Scholar 

  • Srinivasa Moorthy, K., Kasi Reddy, B., Swami, K. S., and Chetty, C. S.: 1986, ‘Dichlorvos induced metabolic changes in tissues of freshwater mussel, Lamellidens marginalis’, J. Environ. Biol. 7, 101–106.

    Google Scholar 

  • Stephenson, R. R.: 1982, ‘Aquatic toxicology of cypermethrin. I. Acute toxicity to some freshwater fish and invertebrates in laboratory tests’, Aqua. Toxicol. 2, 175–185.

    Google Scholar 

  • Vijverberg, H. P. M. and Bercken, J. V. D.: 1982, ‘Action of pyrethroid insecticides on the vertebrate nervous system’, Neuropathol. Appl. Neurobiol. 421–428.

  • Vijverberg, H. P. M. and Bercken, J. V. D. 1990, ‘Neurotoxicological effects and the mode of action of pyrethroid insecticides’, Crit. Rev. Toxicol. 21, 105–126.

    Google Scholar 

  • Wekell, M. M. and Brown, G. W.: 1973, ‘Ornithine aminotransferases of fishes’, Comp. Biochem. Physiol. 46, 779–795.

    Google Scholar 

  • Wouters, W. and Bercken, J. V. D.: 1978, ‘Review: Action of pyrethroids’, Gen. Pharmacol. 9, 387–398.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Reddy, P.M., Bashamohideen, M. Alterations in protein metabolism in selected tissues of fish, Cyprinus carpio, during sublethal concentration of cypermethrin. Environ Monit Assess 36, 183–190 (1995). https://doi.org/10.1007/BF00546789

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00546789

Keywords

Navigation