Summary
In strain IGC 4052 of the amylolytic yeast Lipomyces kononenkoae growing in starch-limited chemostat cultures the critical dilution rate was reduced to about half of its theoretical value due to severe catabolite repression of amylase formation while its value in a repression-resistant mutant was near its theoretical value. The enzyme yield coefficients and the specific production rates of α-amylase and glucoamylase passed through maxima at intermediate dilution rates. The shapes of the respective curves were partly determined by catabolite repression (parent strain) or its absence (mutant strain) while induction did not seem to play to role. An additional growth-linked regulatory mechanism seemed to be involved. The use of continuous culture as compared with batch culture, increased the maximum biomass productivity by a factor of 2.2 in the mutant strain and by a factor of 1.4 in the parent strain.
Similar content being viewed by others
References
Augustin J, Zemek J, Kocková-Kratochvílová A, Kunika L (1978) Production of α-amylase by yeasts and yeast-like organisms. Folia Microbiol 23:353–361
Azoulay E, Jouanneau F, Bertrout JC, Raphael A, Janssens J, Lebault JM (1980) Fermentation methods for protein enrichment of cassava and corn with Candida tropicalis. Appl Environ Microbiol 39:41–47
Clarke P, Houldsworth M, Lilly MD (1968) Catabolite repression and the induction of amidase synthesis by Pseudomonas aeruginosa 8602 in continuous culture. J Gen Microbiol 51:225–234
Dean ACR (1972) Influence of environment on the control of enzyme synthesis. J Appl Chem Biotechnol 22:245–259
Dubois M, Giles UA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Henderson C, Hobson PN, Summers R (1969) The production of amylase, protease and lipolytic enzymes by two species of anaerobic rumen bacteria. In: Malék, Beran, Fencl, Munk, Ribica, Smrbková (eds) Continuous cultivation of microorganisms. Academia, Prague, pp 189–204
Herbert D (1958) Some principles of continuous culture. In: Tunevall G (ed) Recent progress in microbiology. VII International Congress for Microbiology. Almquist & Wiksell, Stockholm, p 381
Hernandez E, Pirt J (1975) Kinetics of utilisation of a highly polimerised carbon source (starch) in a chemostat culture of Klebsiella aerogenes: pullulanase and α-amylase activites. J Appl Chem Biotechnol 25:297–304
Hiromi K, Shibaoka T, Fukube H, One S (1969) Quantitative determination of anomeric forms of sugar produced by amylases. IV. Anomeric form of maltose produced from phenyl α-maltoside catalysed by saccharifying α-amylase from Bacillus subtilis. J Biochem 66:63
Lodder J (1970) The Yeasts, 2nd edn. North Holland Publishing Co, Amsterdam London
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Pirt SJ (1975) Principles of microbe and cell cultivation. Blackwell Scientific Publication, Oxford London Edinburgh Melbourne
Spencer-Martins I, van Uden N (1977) Yields of yeast growth on starch. Eur J Appl Microbiol 4:29–35
Spencer-Martins I, van Uden N (1979) Extracellular amylolytic system of the yeast Lipomyces kononenkoae. Eur J Appl Microbiol Biotechnol 6:241–250
van Uden N (1967) Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168
van Uden N, Cabeca-Silva C, Madeira Lopes A, Spencer-Martins I (1980) Selective isolation of derepressed mutants of an α-amylase yeast by the use of 2-deoxyglucose. Biotechnol Bioeng 22:651–654
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sá-Correia, I., van Uden, N. Production of biomass and amylases by the yeast Lipomyces kononenkoae in starch-limited continous culture. European J. Appl. Microbiol. Biotechnol. 13, 24–28 (1981). https://doi.org/10.1007/BF00505337
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00505337