Summary
The localization of bombesin- (BOMB) and enkephalin-(ENK) immunoreactive (IR) nerves was studied in rat coeliac-superior mesenteric ganglion complex in relation to neuropeptide Y (NPY)- and tyrosine hydroxylase (TH)-immunoreactive neurons with an immunofluorescence double-staining method. Very dense networks of BOMB-IR nerve terminals surrounded the majority of the principal ganglion cells, wheter or not they were TH-IR. BOMB-IR nerves were specifically related to the non-NPY-IR neurons. Moderately dense networks of ENK-IR fibers were unevenly distributed among the ganglion cells. Majority of these neurons exhibited TH-IR and some of them also contained NPY-IR. In sections double stained with antibodies to ENK and BOMB some nerve fibers contained both peptides. The findings suggest that BOMB-IR nerves, which have been previously demonstrated to originate from gut, control the function of non-NPY-IR ganglion cells. ENK-IR nerves apparently control the adrenergic neurons which project to gut and also some NPY-IR vasomotoric neurons. The finding that ENK- and BOMB-IR coexist in some nerves suggests that some ENK-IR nerves may originate from gut, although the major part probably represents preganglionic fibers originating from spinal cord.
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References
Anastasi A, Erspamer V, Bucci M (1971) Isolation and structure of bombesin and alytesin, two analogous active peptides from the skin of the European amphibians Bombina and Alytes. Experientia 27:166–167
Costa M, Furness JB (1984) Somatostatin is present in a subpopulation of noradrenergic nerve fibres supplying the intestine. Neuroscience 13:911–920
Dalsgaard C-J, Hökfelt T, Elfvin L-G, Terenius L (1982) Enkephalin-containing sympathetic preganglionic neurons projecting to the inferior mesenteric ganglion: evidence from combined retrograde tracing and immunohistochemistry. Neuroscience 7:2039–2050
Dalsgaard C-J, Hökfelt T, Schultzberg M (1983) Origin of peptide containing fibers in the inferior mesenteric ganglion of the guinea pig: Immunohistochemical studies with antisera to substance P, enkephalin, vasoactive intestinal polypeptide, cholecystokinin and bombesin. Neuroscience 9:191–211
Fuxe K, Agnati LF, McDonald T, Locatelli V, Hökfelt T, Dalsgaard C-J, Battistini N, Yanaihara N, Mutt V, Cuello AC (1983) Immunohistochemical indications of gastrin releasing peptide-bombesin like immunoreactivity in the nervous system of the rat. Codistribution with substance P-like immunoreactive nerve terminal systems and coexistence with substance P-like immunoreactivity in dorsal root ganglion cell bodies. Neurosci Lett 37:17–22
Hamaji M, Kawai Y, Kawashima Y, Tohyama M (1987) Projections of bombesin-like immunoreactive fibers from the rat stomach to the celiac ganglion revealed, by a double-labeling technique. Brain Res 416:192–194
Helen P, Panula P, Yang H-Y T, Rapoport SI (1984) Bombesin/gastrin-releasing peptide (GRP)- and Met5-enkephaline-Arg6-Gly7-Leu8-like immunoreactivities in small intensely fluorescent (SIF) cells and nerve fibers of rat sympathetic ganglia. J Histochem Cytochem 32:1131–1138
Hervonen A, Linnoila I, Pickel VM, Helen P, Pelto-Huikko M, Alho H, Miller RJ (1981) Localization of (Met5)- and (Leu5)-enkephalin-like immunoreactivity in nerve terminals in human paravertebral sympathetic ganglia. Neuroscience 6:323–330
Jule Y, Clerc N, Niel JP, Condamin M (1986) (Met)- and (Leu)enkephalin-like immunoreactive cell bodies and nerve fibers in the coeliac ganglion of the cat. Neuroscience 18:487–498
Järvi R, Helen P, Pelto-Huikko M, Hervonen A (1986.) Neuropeptide Y (NPY)-like immunoreactivity in rat sympathetic neurons and small granule-containing cells. Neurosci Lett 67:223–227
Kondo H, Kuramoto H, Wainer BH, Yanaihara N (1985) Evidence for the coexistence of acetylcholine and enkephalin in the sympathetic preganglionic neurons of rats. Brain Res 335:309–314
Lindh B, Hökfelt T, Elfvin L-G, Terenius L, Fahrenkrug J, Elde R, Goldstein M (1986) Topography of NPY-, somatostatin- and VIP-immunoreactive neuronal subpopulations in the guinea pig celiac-superior mesenteric ganglion and their projection to the pylorus. J Neurosci 6:2371–2383
Lindh B, Hökfelt T, Elfvin L-G (1988) Distribution and origin of peptide-containing nerve fibers in the celiac superior mesenteric ganglion of the guinea-pig. Neuroscience 26:1037–1071
Lundberg JM, Tatemoto K (1982) Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistent to α-adrenoreceptor blocade. Acta Physiol Scand 116:393–402
Lundberg JM, Terenius L, Hökfelt T, Goldstein M (1983) High levels of neuropeptide Y (NPY) in peripheral noradrenergic neurons in various mammals including man. Neurosci Lett 42:167–172
Macrac IM, Furness JB, Costa M (1986) Distribution of subgroups of noradrenaline neurons in the coelic ganglion of the guinea-pig. Cell Tissue Res 244:173–180
McDonald TJ, Jörnvall H, Nilsson G, Vagne M, Ghatei M, Bloom SR, Mutt V (1979) Characterization of a gastrin releasing peptide from porcine nonantral tissue. Biochem Biophys Res Commun 90:227–233
Panula P, Hadjiconstantinou M, Yang H-YT, Costa E (1983) Immunohistochemical localization of bombesin/gastrin releasing peptide and substance P in primary sensory neurons. J Neurosci 3:2021–2029
Roth KA, Weber E, Barchas JD (1982) Distribution of gastrin releasing peptide-bombesin-like immunostaining in rat brain. Brain Res 251:277–282
Schultzberg M (1983) Bombesin-like immunoreactivity in sympathetic ganglia. Neuroscience 8:363–374
Schultzberg M, Dalsgaard C-J (1983) Enteric origin of bombesin immunoreactive fibers in the rat coeliac-superior mesenteric ganglion. Brain Res 269:190–195
Schultzberg M, Hökfelt T, Terenius L, Elfvin L-G, Lundberg J-M, Brandt J, Elde P, Goldstein M (1979) Enkephalin immunoreactive nerve fibers and cell bodies in sympathetic ganglia of the guinea-pig and rat. Neuroscience 4:249–270
Schultzberg M, Hökfelt T, Nilsson G, Terenius L, Rehfeld JF, Brown M, Elde R, Goldstein M, Said SI (1980) Distribution of peptide and catecholamine-containing neurons in the gastrointestinal traact of rat and guinea pig: Immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostain, gastrin/cholecystokinin, neurotensin and dopamine b-hydroxylase. Neuroscience 5:689–744
Senba E, Shiosaka S, Hara Y, Inagaki S, Sakanaka M, Takatsuki K, Kawai Y, Tohyama M (1982) Ontogeny of the peptidergic system in the rat spinal cord: Immunohistochemical analysis. J Comp Neurol 208:54–66
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Järvi, R. Localization of bombesin-, neuropeptide Y-, enkephalin-and tyrosine hydroxylase-like immunoreactivities in rat coeliac-superior mesenteric ganglion. Histochemistry 92, 231–236 (1989). https://doi.org/10.1007/BF00500923
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DOI: https://doi.org/10.1007/BF00500923