Abstract
An electrophoretic procedure for separating the molecular forms of catechol-O-methyltransferase in cellulose acetate gel is described; the zones of enzyme activity were revealed by autoradiography. The electrophoretic patterns of the enzyme in several tissues and cell lines derived from four different species are presented.
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This investigation was supported by Grant 500.6/Ric. 70/1981 from the Italian Ministry of Health and by the Italian Ministry of Education. We are grateful to Dr. M. Castagnola for useful advice and help with thin-layer chromatography.
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Brahe, C., Crosti, N., Khan, P.M. et al. Catechol-O-methyltransferase: A method for autoradiographic visualization of isozymes in Cellogel. Biochem Genet 22, 125–132 (1984). https://doi.org/10.1007/BF00499292
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DOI: https://doi.org/10.1007/BF00499292