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On using cis-Pt (II)-uracil as a stain for nucleic acids in brain slices and subcellular fractions

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Summary

Chick brain cortical slices and crude mitochondrial fractions were fixed with glutaraldehyde, stained only with cis-Pt (II)-uracil and processed for electron microscopy. The optimal time of staining was determined to be 10 min. Results show that this platinum-pyrimidine complex is a relatively specific stain for the nucleic acids of brain slices. However, staining of crude mitochondrial fractions apparently resulted in some protein staining and other artifacts. The method should be helpful identifying ribosomal contamination of subcellular preparations and if its specificity can be increased it may prove a useful addition to staining methods of the electron microscopist.

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References

  1. Aggarwal, S.K.: Surface binding of platinum-pyrimidine complexes and the nucleic acids in the solid human tumors. J. Cell Biol. 67, 3a (1975)

    Google Scholar 

  2. Aggarwal, S.K., McAllister, P., Wagner, R.W., Rosenberg, R.: Platinum compounds as stains for electron microscopy. Proc. Annual Meeting Electron Microscopy Society of America 32, 230–231 (1974)

    Google Scholar 

  3. Aggarwal, S.K., Wagner, R.W., McAllister, P.K., Rosenberg, R.: Cell-surface-associated nucleic acid in tumorigenic cells made visible with platinum-pyrimidine complexes by electron microscopy. Proc. nat. Acad. Sci. USA (Wash.) 72, 928–932 (1975)

    Google Scholar 

  4. Babitch, J.A., Breithaupt, T.B., Chiu, T.C., Garadi, R., Helseth, D.L.: Preparation of chick brain synaptosomes and synaptosomal membranes. Biochim. biophys. Acta (Amst.) 433, 75–89 (1976)

    Google Scholar 

  5. Caulfield, J.B.: Effects of varying the vehicle for osmium tetroxide in tissue fixation. J. biophys. biochem. Cytol. 3, 827–829 (1957)

    Google Scholar 

  6. Cotman, C.W., Banker, G., Churchill, L., Taylor, D.: Isolation of postsynaptic densities from rat brain. J. Cell Biol. 63, 441–455 (1974)

    Google Scholar 

  7. Deanin, G.G., Gordon, M.W.: Chloramphenacol and cycloheximide sensitive protein synthetic systems in brain mitochondrial and nerve-ending preparations. J. Neurochem. 20, 55–68 (1973)

    Google Scholar 

  8. Dunkley, P.R., Carnegie, P.R.: Amino acid sequence of the smaller basic protein from rat brain myelin. Biochem. J. 141, 243–255 (1974)

    Google Scholar 

  9. Dunn, A.J., Bondy, S.C.: Functional chemistry of the brain. Flushing, N.Y.: Spectrum Publications Inc. 1974

    Google Scholar 

  10. Gordon, M.W., Deanin, G.G.: Protein synthesis by isolated rat brain mitochondrial and synaptosomes. J. biol. Chem. 243, 4222–4226 (1968)

    Google Scholar 

  11. Hamberger, A., Blomstrand, C., Lehninger, A.L.: Comparative studies on mitochondria isolated from neuron-enriched and glia-enriched fractions of rabbit and beef brain. J. Cell Biol. 45, 221–234 (1970)

    Google Scholar 

  12. Kellenberger, E., Ryter, A., Sechaud, J.: Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol. 4, 671–678 (1958)

    Google Scholar 

  13. Norton, W.T., Poduslo, S.E.: Myelination in rat brain: Method of myelin isolation. J. Neurochem. 21, 749–757 (1973)

    Google Scholar 

  14. Ovtscharoff, W.: Cytochemistry and morphology of synaptic structures in the cerebral cortex of rat. Histochemistry 44, 485–492 (1975)

    Google Scholar 

  15. Roberts, J.J., Pascoe, J.M.: Crosslinking of complementary strands of DNA in mammalian cells by antitumor platinum compounds. Nature (Lond.) 235, 282–284 (1972)

    Google Scholar 

  16. Rosenberg, B.: Platinum coordination complexes in cancer chemotherapy. Naturwissenschaften 60, 399–406 (1973)

    Google Scholar 

  17. Rosenberg, B.: Possible mechanisms for the antitumor activity of platinum coordination complexes. Cancer Chemother. Rep. 59, 589–598 (1975)

    Google Scholar 

  18. Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy.J. Ultrastruct. Res. 26, 31–43 (1969)

    Google Scholar 

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Authors and Affiliations

  1. Chemistry of Behavior Program and Department of Chemistry, Texas Christian University, 76129, Fort Worth, Texas, USA

    Joseph A. Babitch, Donald L. Helseth & Tien-Cheng Chiu

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  1. Joseph A. Babitch
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  2. Donald L. Helseth
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  3. Tien-Cheng Chiu
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Additional information

Supported by NIH Grant NS-12485, Grant P-593 from the Robert A. Welch Foundation, and Grant C-7561 from the TCU Research Foundation

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Babitch, J.A., Helseth, D.L. & Chiu, TC. On using cis-Pt (II)-uracil as a stain for nucleic acids in brain slices and subcellular fractions. Histochemistry 49, 253–261 (1976). https://doi.org/10.1007/BF00492381

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  • DOI: https://doi.org/10.1007/BF00492381

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