Abstract
The linkage relationships of 11 loci concerned with protein or enzyme variation in the inbred mouse (Mus musculus) have been investigated. By means of a three-point cross, the order of the loci glucosephosphate isomerase (Gpi-1), albino (c), and hemoglobin β-chain in linkage group I has been established as Gpi-c-Hbb. Similarly, the order of the loci autosomal glucose 6-phosphate dehydrogenase (Gpd-1), misty (m), and brown (b) in linkage group VIII has been established as Gpd-m-b. The levulinate dehydratase locus (Lv) in linkage group VIII which shows 5±2% recombination with the brown locus is near the anemia locus (an). The locus for malic dehydrogenase (Mdh-1) shows 10.1±2.9% recombination with the dilute locus and 12.0±6.5% recombination with the luxoid locus. The tentative order of the three loci is d-Mdh-1-lu. Recombination between the isocitric dehydrogenase locus (Id-1) and the leaden locus (ln) is 16.7±5.8% and between Id-1 and the splotch locus (Sp) is 11.0±5.4% in linkage group XIII. The tentative order of the three loci is ln-Sp-Id-1. Recombination between the lactic dehydrogenase regulatory locus (Ldr-1) and the microphthalmia locus (Mi wh) in linkage group XI is 28.7±4.4%. Recombination between the phosphoglucomutase locus (Pgm-1) and the W-locus in linkage group XVII is 3.0±1.7%. The esterase-3 locus has not been placed in a linkage group and has been tested against markers on linkage groups I, II, III, IV, V, VI, VIII, XI, XII, XIII, XVI, XVII, XVIII, and XX. In no case was there physical linkage of structural genes whose products participate in related metabolic pathways.
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To Dr. Margaret M. Dickie—in memoriam.
Supported by the Roche Institute of Molecular Biology and AEC contract AT (30-1)-3671 with The Jackson Laboratory. The principles of laboratory animal care as promulgated by the National Society for Medical Research were observed.
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Hutton, J.J., Roderick, T.H. Linkage analyses using biochemical variants in mice. III. Linkage relationships of eleven biochemical markers. Biochem Genet 4, 339–350 (1970). https://doi.org/10.1007/BF00485782
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DOI: https://doi.org/10.1007/BF00485782