Abstract
Putrescine transport was examined in normal and cystic fibrosis fibroblasts. No differences were observed in accumulation pattern, kinetics of uptake, or efflux between CF and normal cells. In both growing and growth-arrested CF and normal fibroblasts, exogenously supplied putrescine remained unchanged for at least 60 min. Some differences were observed in the response of CF and normal cells to environmental (media) changes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arvanitakis, S. N., Mangos, J. A., McSherry, N. R., Rennert, O. M., and LaPointe, D. (1973). Role of polyamines in cystic fibrosis. Pediat. Res. 7336.
Baker, A. P., and Hellegrass, L. M. (1974). Effects of polyamines on glycosyltransferases. Fed. Proc. 331300.
Benke, P. J., Erbstoeszer, M., and Pitot, H. C. (1972). Transport of labelled compounds in control and cystic fibrosis cells in vitro. Lancet 1182.
Bose, S. K., and Zlotnick, B. J. (1973). Growth and density-dependent inhibition of deoxyglucose transport in Balb 3T3 cells and its absence in cells transformed by murine sarcoma virus. Proc. Natl. Acad. Sci. 702374.
Brown, G. H., Oshin, A., Goodchild, M. C., and Anderson, C. M. (1971). Inhibition of sugar transport by plasma from cystic fibrosis patients. Lancet 2639.
Cohen, L. F., Farrell, P. M., Willison, J. W., and Lundgren, D. W. (1975). Localization of spermidine (Spd) and spermine (Spm) in blood of cystic fibrosis (CF) and control subjects. Pediat. Res. 9312.
Fletcher, D. S., and Lin, T. Y. (1973). Incorporation of l-leucine and d-glucosamine into skin fibroblasts derived from CF and normal individuals. Clin. Chim. Acta 445.
Johnson, H. G., and Bach, M. K. (1968). Uptake and subcellular localization of tritiated spermine in Escherichia coli. Arch. Biochem. Biophys. 128113.
Kano, K., and Oka, T. (1976). Polyamine transport and metabolism in mouse mammary gland: General properties and hormonal regulation. J. Biol. Chem. 2512795.
Kletzien, R. F., and Perdue, J. F. (1974). Sugar transport in chick embryo fibroblasts. I. Functional change in the plasma membrane associated with the rate of cell growth. J. Biol. Chem. 2493366.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193265.
Lundgren, D. W., Farrell, P. M., and diSant'Agnese, P. A. (1975). Polyamine alterations in blood of male homozygotes and heterozygotes for cystic fibrosis. Clin. Chim. Acta 62357.
McEvoy, F. A., and Hartley, C. B. (1975). Polyamines in cystic fibrosis. Pediat. Res. 9721.
Morton, H. J. (1970). A survey of commercially available tissue culture media. In Vitro 689.
Munro, G. F., Hercules, K., Morgan, J., and Sauerbier, W. (1972). Dependence of the putrescine content of Escherichia coli on the osmotic strength of the medium. J. Biol. Chem. 2471272.
Munro, G. F., Bell, C. A., and Lederman, M. (1974). Multiple transport components for putrescine in Escherichia coli. J. Bacteriol. 118952.
Pohjanpelto, P. (1976). Putrescine transport is greatly increased in human fibroblasts initiated to proliferate. J. Cell Biol. 68512.
Rennert, O. M., Frias, J., and La Pointe, D. (1973). Methylation of RNA and polyamine metabolism in cystic fibrosis. In Mangos, J. A., and Talamo, R. C. (eds.), Fundamental Problems of Cystic Fibrosis and Related Diseases, Intercontinental Medical Book Corp., New York.
Rosenthal, S. M., and Tabor, C. W. (1956). The pharmacology of spermine and spermidine distribution and excretion. J. Pharmacol. Exp. Ther. 116131.
Schneider, E. L., Stanbridge, E. J., and Epstein, C. J. (1974). Incorporation of 3H-uridine and 3H-uracil for the detection of mycoplasma contamination of cultured cells. Exp. Cell Res. 84311.
Sullivan, J. L., Kelly, J. C., Roess, W. B., and DeBusk, A. G. (1976). Methionine transport in fibroblasts from cystic fibrosis patients. In preparation.
Tabor, C. W., and Tabor, H. (1966). Transport systems for 1,4-diaminobutane, spermidine and spermine in Escherichia coli. J. Biol. Chem. 2413714.
Tabor, H., and Tabor, C. W. (1964). Spermidine, spermine and related amines. Pharmacol. Rev. 16245.
Tabor, H., and Tabor, C. W. (1972). Biosynthesis and metabolism of 1,4-diaminobutane, spermidine, spermine and related amines. Adv. Enzymol. 36203.
Taussig, L. M., and Gardner, J. D. (1972). Effects of saliva and plasma of CF patients on membrane transport. Lancet 11367.
Williams-Ashman, H. G., and Lockwood, D. H. (1970). Role of polyamines in reproductive physiology and sex hormone action. Ann. N.Y. Acad. Sci. 171882.
Author information
Authors and Affiliations
Additional information
This research was supported by a grant from the Cystic Fibrosis Foundation and by a grant from the National Institutes of Health, Training Grant (GM01316 11 GNC).
Rights and permissions
About this article
Cite this article
Kelly, J.C., DeBusk, A.G. Membrane function in cystic fibrosis. I. Putrescine transport in normal and cystic fibrosis fibroblasts. Biochem Genet 15, 695–705 (1977). https://doi.org/10.1007/BF00484098
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00484098