Abstract
In vitro phagocytosis of thirteen Candida berkhout species by rat leukocytes was studied to assess a possible correlation between pathogenicity and phagocytosis
Yeast-leukocyte suspensions were mixed up for 3 h and phagocytic index, germ-tube formation and leukocyte candidacidal activity were evaluated. Highest values for phagocytosis were reached in all cases at the end of the first hour. Leukocyte candidacidal activity was absent. Only C. albicans produced germ-tubes.
The various phagocytosis indices were determined depending on the Candida species assayed. Under these conditions, the more pathogenic species presented the lower indices of phagocytosis.
It is determined that the in vitro phagocytic index may bear a close relationship with the pathogenicity of the Candida berkhout.
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Arai, T., Y. Mikami & K. Yokoyama, 1977. Phagocytosis of C. albicans by rabbit alveolar macrophages and guinea pig neutrophils. Sabouraudia 15: 171–177.
Borghi, A. L. & B. C. de Bracalenti, 1971. II Fagocitosis en leucocitos de ratón in vivo e in vitro. Actas II Jornadas Argentinas de Micologia 145–148.
Evron, R., 1980. In vitro phagocytosis of C. albicans by Peritoneal mouse macrophages. Infect. Immun. 28: 963–971.
Lehrer, R. I. & M. J. Cline, 1969. Interaction of Candida albicans with human leukocytes and serum. J. Bacteriol. 98: 996–1004.
Lehrer, R. I. & M. J. Cline, 1969. Leukocyte myeloperoxidase deficiency and disseminated candidiasis. The role of myeloperoxidase in resistance to Candida infection. J. Clin. Invest. 48: 1478
Leijh, P. C., M. T. Van Den Barselaar & R. Van Furth, 1977. Kinetics of phagocytosis and intracellular killing of Candida albicans by human granulocytes and monocytes. Infect. Inmun. 17:313–318.
Louria, D. B. & R. G. Brayton, 1964. Behavior of Candida cells within leukocytes. Proc. Soc. Exp. Biol. Med. 115: 93–98.
Margni, R., 1982. Inmunología e Inmunoquímica. Editorial Medica Panamericana S.A. Bs. As. Argentina. 3rd. Ed.: pp. 447.
Morelli, R. & L. T. Rosenberg, 1971. The role of complement in the phagocytosis of Candida albicans by mouse peripheral blood leukocytes. J. Immunol. 107: 476–482.
Ozato, K. & I. Uesaka, 1974. The role of macrophages in C. albicans infection in vitro. Jpn. J. Microbiol. 18: 29–35.
Stanley, V. C. & R. Hurley, 1969. The growth of Candida species in cultures of mouse peritoneal macrophages. J. Pathol. 97: 357–366.
Van Furth, R., T. L. Van Zwet & P. C. Leijh, 1977. In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes. In D. M. Weir (ed.), Handbook of Experimental Immunology, J. B. Lippincott Co., New York, 3rd ed.
Van Gelderen de Komaid, A., J. Silva & F. Elías, 1980. Las onicomicosis candidiasicas en Tucumán. Clasificación de especies según N. van Uden y H. Buckley, 1971. Rev. Arg. Micol. 3(1): 5–8.
Venkataraman, M., L. N. Mohapatra & U. N. Bhoyan, 1973. Phagocytosis of Candida albicans by rabbit neutrophils. Sabouraudia 9: 183–191.
Verhaegen, H., W. De Cock & J. De Cree, 1976. In vitro phagocytosis of Candida albicans by peripheral polymorphonuclear neutrophils of patients with recurrent infections. Case reports of serum-dependent abnormalities. Biomedicine 24: 164–170.
Wang, C. J. & Y. Schwarz, 1959. Phagocytosis of yeast cells in vitro. Amer. J. Path. 35: 901
Young, G., 1958. The process of invasion and the persistence of C. albicans injected intraperitoneally into mice. J. Infect. Dis. 102: 114–120.
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de Petrino, S.E.F., de Jorrat, M.E.B.B. & Sirena, A. In vitro phagocytosis of several candida berkhout species by murine leukocytes. Mycopathologia 89, 139–145 (1985). https://doi.org/10.1007/BF00447022
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DOI: https://doi.org/10.1007/BF00447022