Abstract
The measurement of cerebral blood flow (CBF) by continuous C15O2 inhalation has only been validated previously by indirect experimental protocols. In the present study using baboons, these measurements were compared directly with those obtained by injection of 68Ga-labelled serum-albumin microspheres in the left cardiac ventricle. Using a modified labelling technique, no elution of 68Ga occurred in vivo. Both methods provided similar regional CBF values, which could be described by a significant linear correlation (\(CBF_{CO_2 }\)=0.82 CBFmicrospheres+5.7; P<0.001). The validity of the labelled-microsphere-injection method was verified. The feasibility of stable in vivo labelling of 68Ga to serum-albumin microspheres provides a reference method for organ blood-flow measurements using positron-emission tomography.
Similar content being viewed by others
References
Baron JC, Comar D, Bousser G, Soussaline F, Crouzel C, Plummer D, Kellershohn C, Castaigne P (1978) Etude tomographique chez l'homme, du débit sanguin et de la consommation d'oxygène du cerveau par inhalation continue d'oxygène 15. Rev Neurol (Paris) 134:545–556
Baron JC, Naquet R, Steinling M (1980) Tomographic measurement of cerebral blood flow with the C15O2 continuous inhalation technique: experimental evidence. Ann Neurol 8:99–100
Baron JC, Steinling M, Tanaka T, Cavalheiro E, Soussaline F, Collard P (1981) Quantitative measurement of CBF, oxygen extraction fraction (OEF) and CMRO2 with the 15-O continuous inhalation technique and positron emission tomography (PET): experimental evidence and normal values in man. J Cereb Blood Flow Metab 1 (suppl 1):5–6
Blasco A (1980) Etude expérimentale de la débitmétrie sanguine par la méthode des microsphères marquées. Mémoire DERBH, Bordeaux
Buckberg G, Luck Z, Payne D (1969) Some sources or error in measuring regional blood flow with radioactive microspheres. J Appl Physiol 27:296–300
Depresseux JC, Cheslet JP, Hodiaumont J (1982) Evaluation tomographique chez l'homme du débit sanguin cérébral et du volume cérébral d'eau rapidement échangeable. J Biophys Med Nucl 6:173–177
Eichling J, Raichle M, Grubb R, Ter Pogossian MM (1974) Evidence of the limitations of water as a freely diffusible tracer in brains of the rhesus monkey. Circ Res 35:358–364
Herscovitch P, Markham J, Raichle ME (1983) Brain blood flow measurement with intravenous H2 15O. J Nucl Med 24:782–789
Heymann M, Payne B, Hoffman J, Rudolph A (1977) Blood flow measurements with radionuclide labeled particles. Proc Cardiovasc Dis 20:55–77
Huang SC, Carson RE, Hoffman EJ, Barrio J, Phelps M (1983) Quantitative measurement of local cerebral blood flow in humans by positron computed tomography and 15O water. J Cereb Blood Flow Metab 3:141–153
Jones T, Chesler DA, Ter Pogossian MM (1976) The continuous inhalation of oxygen 15 for assessing regional oxygen extraction in the brain of man. Br J Radiol 49:339–343
Kanno I, Lammertsma AA, Heather JD, Gibbs JM, Rhodes CG, Clark JC, Jones T (1984) Measurement of cerebral blood flow using bolus inhalation of C15O2 and positron emission tomography: description of the method and its comparison with the C15O2 continuous inhalation method. J Cereb Blood Flow Metab 4:224–234
Lammertsma A, Jones T, Frackowiak R, Lenzi G (1981) A theoretical study of the steady state method for measuring regional cerebral blood flow and oxygen utilisation using oxygen 15. J Comput Assist Tomogr 5:544–550
Loc'h C, Mazière B, Comar D (1980) A new generator for ionic gallium 67. J Nucl Med 21:171–173
Marcus M, Heistad D, Ehrhardt J, Abbout F (1976) Total and regional cerebral blood flow measurement with 7, 10, 15, 25 and 50 μm microspheres. J Appl Physiol 40:501–507
Marcus M, Bischof C, Heistad D (1981) Comparison of microsphere and xenon 133 clearance method in measuring skeletal muscle and cerebral blood flow. Cir Res 48:748–761
Ofjord ES, Clausen G (1983) Intrarenal flow of microspheres and red blood cells: skimming in slit and tube models. Am J Physiol 245:H429-H436
Raichle ME, Martin WR, Herscovitch P, Mintun MA, Markhan J (1983) Brain blood flow measurement with intravenous H2 15O. J Nucl Med 24:790–798
Rhodes C, Lenzi G, Jones T (1981) Measurement of CBF and CMRO2 using the continuous inhalation of C15O2 and 15O2. J Neurol Sci 50:381–389
Steinling M, Baron JC (1982) Mesure du débit sanguin cérébral local par inhalation continue de C15O2 et tomographie d'émission: discussion du modèle. J Bioph Med Nucl 6:89–96
Steinling M, Baron JC, Soussaline F, Rousseau J, Comar D, Naquet R, Kellershohn C (1983) Diffusion de l'eau et mesure du D.S.C. par inhalation de C15O2. Int J Med Biol 10:17–22
Wagner HN Jr, Rhudes BA, Sasaki Y (1969) Studies of the circulation with radioactive microspheres. Invest Radiol 4:374–386
Wisemberg G, Schelberg H, Hoffman E (1981) In vivo quantification of regional myocardial blood flow by positron-emission computed tomography. Circulation 62:1248–1258
Yvert JP, Mazière B, Berhas H, Comar D (1979) Simple, fast preparation of gallium 68 labelled human albumin microspheres. Eur J Nucl Med 4:95–99
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Steinling, M., Baron, J.C., Maziere, B. et al. Tomographic measurement of cerebral blood flow by the 68Ga-labelled-microsphere and continuous-C15O2-inhalation methods. Eur J Nucl Med 11, 29–32 (1985). https://doi.org/10.1007/BF00440957
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00440957