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Genetic transformation of Pseudomonas phaseolicola by R-factor DNA

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Summary

The bacterium Pseudomonas phaseolicola was successfully transformed, for the first time, with R-factors RSF1010 and pBR322 DNA by a calciumshock and heat-pulse technique. Frequency of transformation for RSF1010 ranged from 0.8×10-7 to 3.1×10-6 and was ca. 0.4×10-8 for pBR322.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Hawaii, 96822, Honolulu, Hawaii, USA

    Basappa V. Gantotti & Suresh S. Patil

  2. Department of Biochemistry and Biophysics, University of Hawaii, 96822, Honolulu, Hawaii, USA

    Morton Mandel

Authors
  1. Basappa V. Gantotti
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  2. Suresh S. Patil
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  3. Morton Mandel
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Communicated by B.A. Bridges

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Gantotti, B.V., Patil, S.S. & Mandel, M. Genetic transformation of Pseudomonas phaseolicola by R-factor DNA. Molec. Gen. Genet. 174, 101–103 (1979). https://doi.org/10.1007/BF00433310

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  • DOI: https://doi.org/10.1007/BF00433310

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