Abstract
Strain S-36, a marine Pseudomonas sp., was grown under manganese limitation in continuous culture. At dilution rates below a maximal growth rate of 0.066 h-1, the rate at which the organism fixed CO2 into macromolecules was equal to the cell carbon production rate. In addition, the total amount of cell carbon or CO2 fixed at steady-state was in proportion to the amount of energy available from the oxidation of Mn2+ in the medium. These data suggest that the organism can grow by obtaining the energy for CO2 fixation from manganese oxidation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ali SH, Stokes JL (1971) Stimulation of heterotrophic and autotrophic growth of Sphaerotilus discophorus by manganese ions. Antonie von Leeuwenhoek J Microbiol Serol 37:519–528
Beijerinck MW (1913) Oxydation des Manganbikarbonates durch Bakterien und Schimmelpilze. Folia Microbiol (Delft) 2:123–134
Bowden WB (1977) Comparison of two direct-count techniques for enumerating aquatic bacteria. Appl Environ Microbiol 33:1229–1232
Brewer PG, Spencer DW (1971) Colorimetric determination of manganese in anoxic waters. Limnol Oceanogr 16:107–110
Bromfield SM, David O (1976) Sorption and oxidation of manganous ions and reduction of manganese oxide by cell suspensions of a manganese-oxidizing bacterium. Soil Biol Biochem 8:37–43
Eccleston M, Kelly DP (1978) Oxidation kinetics and chemostat growth kinetics of Thiobacillus ferroxidans on tetrathionate and thiosulfate. J Bacteriol 134:718–727
Ehrlich HL (1976) Manganese as an energy source for bacteria. In: Niragu O (ed) Environmental biogeochemistry, vol 2, Metals, transfer and ecological mass balance. Ann Arbor Science Publishers. Ann Arbor, Michigan, pp 633–644
Ehrlich HL (1978) Conditions for bacterial participation in the initiation of manganese deposition around marine sediment particles. In: Krumbein WE (ed) Environmental biogeochemistry and geomicrobiology, vol 3, Methods, metals and assessement. Ann Arbor Science Publishers, Ann Arbor, Michigan, pp 839–845
Ehrlich HL (1982) Enhanced removal of manganese II from sea water by marine sediments and clay minerals in the presence of bacteria. Can J Microbiol 28:1389–1395
Ehrlich HL (1983) Manganese-oxidizing bacteria from a hydrothermally active area on the Galapagos rift. In: Halberg R (ed) Environmental biogeochemistry. Ecol Bull (Stockholm) 35:357–366
Ehrlich HL (1985) Mesophilic manganese-oxidizing bacteria from hydrothermal discharge areas at 21° North on the East Pacific Rise. In: Caldwell DE, Brierly JA, Brierly CA (eds) Planetary ecology. Van Nostrand Reinhold, New York, pp 186–194
Hempfling WP, Vishniac W (1967) Yield coefficients of Thiobacillus neapolitanus in continuous culture. J Bacteriol 93:874–878
Kepkay PE, Burdige DJ, Nealson KH (1984) Kinetics of bacterial manganese binding and oxidation in the chemostat. Geomicrobiol J 3:245–262
Krumbein WE (1971) Manganese oxidizing fungii and bacteria in recent shelf sediments of the Bay of Biscay and the North Sea. Naturwissenschaften 58:56–57
Lieske R (1919) Zur Ernährungsphysiologie der Eisenbakterien. Zentralbl Bakt Infectionskr Hyg II Abt 49:413–425
Luria SE (1960) The bacterial protoplasm: Composition and organization. In: Gunsalus IC, Stanier RY (eds) The bacteria, vol I. Academic Press, New York, pp 107–129
Morgan JJ (1967) Chemical equilibria and kinetic properties of Mn in natural waters. In: Faust SD, Hunter IV (eds) Principles and applications of water chemistry. Wiley, New York, pp 238–251
Nealson KH (1978) The isolation and characterization of marine bacteria which catalyze manganese oxidation. In: Krumbein W (ed) Environmental biogeochemistry, vol 3. Ann Arbor Science Publishers, Ann Arbor, Michigan, 847–858
Pirt SJ (1975) Principles of microbe and cell cultivation. Wiley, New York
Rosenfeld JK (1979) Ammonium adsorption in nearshore anoxic sediments. Limnol Oceanogr 24:356–364
Sartory A, Meyer J (1947) Contribution á l'étude du metabolisme hydrocarbone des bactéries ferrugireuses. C. R. Acad Science, 225:541–542
Schlegel HG (1975) Mechanisms of chemoautotrophy In: Kinne O (ed) Marine ecology, vol II. Wiley Interscience, New York, pp 64–89
Schütt C, Ottow JCG (1978) Distribution and identification of manganese precipitating bacteria from non-contaminated ferromanganese nodules. In: Krumbein WE (ed) Environmental biogeochemistry and geomicrobiology, vol 3, Methods, metals and assessment. Ann Arbor Science Publishers, Ann Arbor, Michigan, pp 869–878
Schwiesfurth R, Eleftheriadis D, Gundlach H, Jacobs M, Jung W (1978) Microbiology of the precipitation of manganese. In: Krumbein WE (ed) Environmental biogeochemistry and geomicrobiology, vol 3, Methods, metals and assessment. Ann Arbor Science Publishers, Ann Arbor, Michigan, pp 923–928
Solorzano L (1969) Determination of ammonia in natural waters by phenolhypochlorite methods. Limnol Oceanogr 14:799–801
Stumm W, Morgan JJ (1981) Aquatic chemistry. Wiley Interscience, New York
Tebo BM (1983) The ecology and ultrastructure of manganese oxidizing bacteria. Ph. D. Thesis, Univ. of California, San Diego
Woese CR, Stackebrandt E, Weisburg WG, Paster BJ, Madigan MT, Fowler VJ, Hahn CM, Blanz P, Gupta R, Nealson KH, Fox GH (1984) The phylogeny of the purple bacteria: The alpha subdivision. Syst Appl Microbiol 5:315–326
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kepkay, P.E., Nealson, K.H. Growth of a manganese oxidizing Pseudomonas sp. in continuous culture. Arch. Microbiol. 148, 63–67 (1987). https://doi.org/10.1007/BF00429649
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00429649