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A dissimilatory nitrite reductase in Paracoccus halodenitrificans

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Abstract

Paracoccus halodenitrificans produced a membrane-associated nitrite reductase. Spectrophotometric analysis showed it to be associated with a cd-cytochrome and located on the inner side of the cytoplasmic membrane. When supplied with nitrite, membrane preparations produced nitrous oxide and nitric oxide in different ratios depending on the electron donor employed. The nitrite reductase was maximally active at relatively low concentrations of sodium chloride and remained attached to the membranes at 100 mM sodium chloride.

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References

  • Cresswell CF, Hageman RH, Hewitt EJ, Hucklesby DP (1965) The reduction of nitrate, nitrite, and hydroxylamine to ammonia by enzymes from Cucurbita pepo L. in the presence of reduced benzyl viologen as electron donor. Biochem J 94:40–53

    Google Scholar 

  • Firestone MK (1982) Biological denitrification. In: Stevenson FJ (ed) Nitrogen in agricultural soils. American Society for Agronomy, Madison, Wisconsin, pp 289–326

    Google Scholar 

  • Iwasaki H, Matsubara T (1971) Cytochrome c-557 (551) and cytochrome cd of Alcaligenes feacalis. J Biochem (Tokyo) 69:847–857

    Google Scholar 

  • Iwasaki H, Matsubara T (1972) A nitrite reductase from Achromobacter cycloclastes. J Biochem 71:645–652

    Google Scholar 

  • Knowles R (1982) Denitrification. Microbiol Rev 46:43–70

    Google Scholar 

  • Kodama T (1970) Effects of growth conditions on formation of cytochrome system of a denitrifying bacterium, Pseudomonas stutzeri. Plant Cell Physiol 11:231–239

    Google Scholar 

  • Koike I, Hattori A (1975) Energy yield of denitrification: an estimate from growth yields in continuous culture of Pseudomonas denitrificans under nitrate, nitrite, and nitrous oxide-limited conditions. J Gen Microbiol 88:11–19

    Google Scholar 

  • Lam Y, Nicholas DJD (1969) A nitrite reductase with cytochrome oxidase activity from Micrococcus denitrificans. Biochim Biophys Acta 180:459–472

    Google Scholar 

  • Le Gall J, Payne WJ, Morgan v, Dervartanian D (1979) On the purification of nitrite reductase from Thiobacillus denitrificans and its reaction with nitrite under reducing conditions. Biochem Biophys Res Comm 87:335–362

    Google Scholar 

  • Lowry O, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Payne WJ (1981) Denitrification. Wiley-Interscience, New York, NY

    Google Scholar 

  • Sadler M, McAninch M, Alico R, Hochstein LI (1980) The intracellular Na+ and K+ composition of the moderately halophilic bacterium Paracoccus halodenitrificans. Can J Microbiol 26:496–502

    Google Scholar 

  • Sawhney V, Nicholas DJD (1978) Sulphide-linked nitrite reductase from Thiobacillus denitrificans with cytochrome oxidase activity: purification and properties. J Gen Microbiol 106:119–128

    Google Scholar 

  • Showe MK, DeMoss JA (1968) Localization and regulation of synthesis of nitrate reductase in Escherichia coli. J Bacteriol 95:1305–1313

    Google Scholar 

  • Wharton DC, Weintraub ST (1980) Identification of nitric oxide and nitrous oxide as products of nitrite reduction by Pseudomonas cytochrome oxidase (nitrite reductase). Biochem Biophys Res Comm 97:236–242

    Google Scholar 

  • Yamanaka T, Okunuki K (1963) Crystalline Pseudomonas cytochrome oxidase. I. Enzymatic properties with special reference to the biological specificity. Biochim Biophys Acta 67:366–378

    Google Scholar 

  • Zumft WG, Sherr BF, Payne WJ (1979) A reappraisal of the nitricoxide binding protein of denitrifying Pseudomonas. Biochem Biophys Res Comm 88:1230–1236

    Google Scholar 

  • Zumft WG, Frunzke K (1982) Discrimination of ascorbateduction of nitric oxide in denitrifying Pseudomonas perfectomarinus. Biochim Biophys Acta 681:459–468

    Google Scholar 

  • Zumft WG, Vega JM (1979) Reduction of nitrite to nitrous oxide by a cytoplasmic membrane fraction from the marine denitrifier Pseudomonas perfectomarinus. Biochim Biophys Acta 548: 484–499

    Google Scholar 

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Grant, M.A., Hochstein, L.I. A dissimilatory nitrite reductase in Paracoccus halodenitrificans . Arch. Microbiol. 137, 79–84 (1984). https://doi.org/10.1007/BF00425812

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  • DOI: https://doi.org/10.1007/BF00425812

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