Skip to main content
SpringerLink
Log in

Characterization of a new murein-associated lipoprotein in the outer membrane of Proteus mirabilis

  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

A murein-associated outer membrane protein from Proteus mirabilis has been isolated. Since the protein carries ester- as well as amide-linked fatty acids it can be classified as a second outer membrane lipoprotein. An apparent molecular weight of 15,000 for this protein was determined from amino acid analysis and sodium dodecylsulfate/polyacrylamide gel electrophoresis. The amino acid composition, however, does not show similarities with the amino acid composition of the lipoprotein covalently linked to murein, which has a molecular weight of 7,300 as described previously in Proteus mirabilis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

SDS:

sodium dodecylsulfate

References

  • Braun V (1975) Covalent lipoprotein from the outer membrane of Escherichia coli. Biochim Biophys Acta 415:335–377

    Google Scholar 

  • Braun V, Rehn K (1969) Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli wall. Eur J Biochem 10:426–438

    Google Scholar 

  • DiRienzo JM, Nakamura K, Inoye M (1978) The outer membrane proteins of gram-negative bacteria: Biosynthesis, assembly and functions. Ann Rev Biochem 47:481–532

    Google Scholar 

  • Gmeiner J (1979) Covalent linkage of lipoprotein to peptidoglycan is not essential for outer membrane stability in Proteus mirabilis. Arch Microbiol 121:177–180

    Google Scholar 

  • Gmeiner J, Martin HH (1976) Phospholipid and lipopolysaccharide in Proteus mirabilis and its stable protoplast l-form. Eur J Biochem 67:487–494

    Google Scholar 

  • Gmeiner J, Kroll HP, Martin HH (1978) The covalent rigid-layer lipoprotein in cell walls of Proteus mirabilis. Eur J Biochem 83:227–233

    Google Scholar 

  • Gmeiner J, Schlecht S (1980) Molecular composition of the outer membrane of Escherichia coli and the importance of protein-lipopolysaccharide interactions. Arch Microbiol. 127:81–86

    Google Scholar 

  • Gruss P, Gmeiner J, Martin HH (1975) Amino-acid composition of the covalent rigid-layer lipoprotein in cell walls of Proteus mirabilis. Eur J Biochem 57:411–414

    Google Scholar 

  • Hantke K, Braun V (1973) Covalent binding of lipid to protein. Diglyceride and amid-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane. Eur J Biochem 34:284–296

    Google Scholar 

  • Hazumi N, Yamada H, Mizushima S (1978) Two new peptidoglycan-associated proteins in the outer membrane of Escherichia coli K-12. FEMS Lett 4:275–277

    Google Scholar 

  • Hirashima A, Wu HC, Venkateswaran PS, Inouye M (1973) Two forms of a structural lipoprotein in the envelope of Escherichia coli. J Biol Chem 248:5654–5659

    Google Scholar 

  • Hirota Y, Suzuki H, Nishimura Y, Yasuda S (1977) On the process of cellular division in Escherichia coli — Mutant of E. coli lacking a murein-lipoprotein. Proc. Natl Acad Sci (Wash) 74:1417–1420

    Google Scholar 

  • Inouye M, Shaw J, Shen C (1972) The assembly of a structural lipoprotein in the envelope of Escherichia coli. J Biol Chem 247:8154–8159

    Google Scholar 

  • Inouye S, Takeishi K, Lee N, DeMartini M, Hirashima A, Inouye M (1976) Lipoprotein from the outer membrane of Escherichia coli: Purification, paracrystallization, and some properties of its free form. J Bacteriol 127:555–563

    Google Scholar 

  • Lugtenberg B, Bronstein H, Van Selm N, Peters R (1977) Peptidoglycan-associated outer membrane proteins in gram-negative bacteria. Biochim Biophys Acta 465:571–578

    Google Scholar 

  • Martin HH, Maskos C, Burger R (1975) d-Alanyl-d-alanine carboxypeptidase in the bacterial form and l-form of Proteus mirabilis. Eur J Biochem 55:465–473

    Google Scholar 

  • Mizuno T (1979) A novel peptidoglycan-associated lipoprotein found in the cell envelope of Pseudomonas aeruginosa and Escherichia coli. J Biochem (Tokyo) 86:991–1000

    Google Scholar 

  • Nixdorff K, Fitzer H, Gmeiner J, Martin HH (1977) Reconstitution of model membranes from phospholipid and outer membrane proteins of Proteus mirabilis. Eur J Biochem 81:63–69

    Google Scholar 

  • Rosenbusch JP (1974) Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding. J Biol Chem 249:8019–8029

    Google Scholar 

  • Yamada H, Mizushima S (1978) Reconstitution of an ordered structure from major outer membrane constituents and the lipoprotein-bearing peptidoglycan sacculus of Escherichia coli. J Bacteriol 135:1024–1031

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie, Fachbereich Biologie, Technische Hochschule Darmstadt, Schnittspahnstr. 9, D-6100, Darmstadt, Federal Republic of Germany

    Jobst Gmeiner

Authors
  1. Jobst Gmeiner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gmeiner, J. Characterization of a new murein-associated lipoprotein in the outer membrane of Proteus mirabilis . Arch. Microbiol. 128, 299–302 (1981). https://doi.org/10.1007/BF00422534

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00422534

Key words

Search

Navigation