Summary
Fragments of DNA with function as autonomous replication sequences in yeast were cloned from Physarum polycephalum. The ars activity is located in a 1.2 kbp fragment extanding 1.5 kbp to 2.7 kbp upstream of the 5′ end of a histone H4 gene. Our recent finding that a replication origin is located at a distance less than 3 kbp of this histone gene suggests that the ars element identified coincides with a specialized replication origin and can be used to direct chromosome replication in Physarum polycephalum.
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Wilhelm, M.L., Demouveau, M.F. & Wilhelm, F.X. Identification of an autonomously replicating sequence near a histone gene of Physarum polycephalum . Curr Genet 10, 459–462 (1986). https://doi.org/10.1007/BF00419874
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DOI: https://doi.org/10.1007/BF00419874