Abstract
The authors show the direct in vitro action of thyroid hormones on RNA-polymerase activity in rat liver mitochondria. 3,5,3′ L-triiodothyronine (L-T3) and 3,5,3′,5′ L-tetraiodothyronine (L-T4) stimulate mitochondrial RNA synthesis without either increasing the permeability of preswollen mitochondria or stimulating the synthesis of the triphosphate ribonucleotides (NTP's). Thyroid hormones do not directly depress mitochondrial RNA hydrolysis. Studies carried out with structural analogues of thyroid hormones indicate the structural specifications of the regulating system of the mitochondrial RNA-polymerase. L-T3 and L-T4 are also effective ‘in vitro’ on mitochondria obtained from animals undergoing different hormonal and dietary treatments, with the exceptions of those fed with a hypoprotein diet. Thus, the authors suggest the possible intervention of a specific mitochondrial receptor for L-T3 and L-T4.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
OppenheimerJ. H., KoernerD., SchawartsH. & Lud SurksM. I., 1972. J. Clin. Endocrinol. Metab. 35: 330–333.
DeGrootL. J. & TorresaniJ., 1975. Endocrinology 96: 357–369.
EdelmanI. S. & Ismail-BeigiF., 1974. Recent Progr. Horm. Res. 30: 235–257.
PrimackM. P., TapleyD. F. & BuchananJ., 1972. Endocrinology 91: 840–844.
DeLeoT., DiMeoS., BarlettaA., MartinoG. & GogliaF., 1976. Pflugers Arch. 366: 73–77.
LiveriniG., MartinoG., BarlettaA., DiMeoS. & DeLeoT., 1976. It. J. Biochem. 25: 115–126.
LiveriniG., MartinoG., BarlettaA., DiMeoS. & DeLeoT., 1976. It. J. Biochem. 25: 236–244.
GadaletaM. N., DiRedaN., BoveG. & SacconeC., 1975. Europ. J. Biochem. 51: 495–501.
SterlingK. & MilchP. O., 1976. In: RobbinsJ. & BravermanL. E. (eds.) Thyroid Research. Excerpta Medica Elsevier, Amsterdam, pp. 342–346.
SterlingK., LazarusJ. H., MilchP. O., SakuradaT. & BrennerM. A., 1978. Science 201: 1126–1129.
SterlingK., 1979(a). N. Engl. J. Med. 300: 117–123.
SterlingK., 1979(b). N. Engl. J. Med. 300: 173–177.
SterlingK., BrennerP. O. & SakuradaT., 1980. Science 210: 340–342.
SterlingK., CampbellG. A., TaliadourosG. S. & NunezE. A., 1984. Cell Tissue Res. 236: 321–325.
RemingtonR. E. & LevineH., 1936. J. Nutr. 11: 343–357.
EvansE. S., SchooleyR. A., EvansA. B., JenkinsC. A. & TaurogA., 1966. Endocrinology 78: 933–1001.
WaddelW. J., 1956. J. Lab. Clin. Med. 48: 311–314.
GadaletaM. N., BarlettaA., CaldarazzoM., DeLeoT. & SacconeC., 1972. Europ. J. Biochem. 30: 376–381.
GadaletaM. N., GrecoM. & SacconeC., 1970. FEBS Lett. 10: 54–56.
Piatnek-LeunissenD. A. & LeunissenR. L. A., 1969. Endocrinology 84: 456–461.
EstabrookR. W. & PullmanM. N., 1967. In: ColowickS. P. & KaplanN. O. (eds.) Methods in Enzimology, Academic Press, New York, Vol. 10, pp. 41–47.
HufnerM. & HeschR. D., 1973. Acta Endocr. 72: 464–474.
BurtonK., 1956. Biochem. J. 62: 315–323.
ObregonM. J., Morreale de EscobarG. & Escobar del ReyF., 1977. Acta Endocr. (Koebvn), 85, Suppl. 212, 172.
Saccone, C., Gadaleta, M. N. & Gimigliano, A. F., 1968. In: Slater, E. C., Tager, J. M., Papa, S. & Quagliariello, E. (eds.) Biochemical Aspects of the Biogenesis of Mitochondria, Adriatica Editrice Bari, pp. 265–278.
GalleraniR. & SacconeC., 1974. In: KroonA. M. & SacconeC. (eds.) The Biogenesis of Mitochondria, Academic Press, New York, pp. 59–69.
Pittman, C. S. & Pittman, J. A., 1974. In: Green, M. A. & Solomon, D. A. (eds.), Thyroid Amer. Physiol. Soc., Washington, Vol. III., pp. 233–253.
RohrA. P., MichelH. J. & BianchiL., 1971. Pathol. Europ. 6: 322–341.
HaradaNaoko, 1967. J. Nutr. 93: 263–272.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Martino, G., Covello, C., De Giovanni, R. et al. Direct in vitro action of thyroid hormones on mitochondrial RNA-polymerase. Mol Biol Rep 11, 205–211 (1986). https://doi.org/10.1007/BF00419598
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00419598