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Molecular organization of the outer membrane of Salmonella typhimurium different release of lipopolysaccharide from wild type and lipopolysaccharide mutant cells by EDTA treatment

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Abstract

Cells of Salmonella typhimurium wild type and of several well defined lipopolysaccharide mutants were treated with EDTA. The percentage release of lipopolysaccharide and phospholipid was determined. The results obtained show that the release of lipopolysaccharide by EDTA declines along with the gradually diminishing chain length of the lipopolysaccharide, althought the total amount of lipopolysaccharide was found to increase at the same time in the respective mutants. Implications of these findings for the organization of the outer membrane are discussed.

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Authors and Affiliations

  1. Technische Hochschule Darmstadt, Institut für Mikrobiologie, Schmittspahnstr. 9, D-6100, Darmstadt, Germany

    Jobst Gmeiner & Hildegard Bergmann

  2. Max-Planck-Institut für Immunbiologie, D-7800, Freiburg, Germany

    Siegfried Schlecht

Authors
  1. Jobst Gmeiner
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  2. Hildegard Bergmann
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  3. Siegfried Schlecht
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Gmeiner, J., Bergmann, H. & Schlecht, S. Molecular organization of the outer membrane of Salmonella typhimurium different release of lipopolysaccharide from wild type and lipopolysaccharide mutant cells by EDTA treatment. Arch. Microbiol. 124, 69–71 (1980). https://doi.org/10.1007/BF00407030

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  • DOI: https://doi.org/10.1007/BF00407030

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