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Effect of nitrogen stress and abscisic acid on nitrate absorption and transport in barley and tomato

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Abstract

The potential of barley (Hordeum vulgare L.) and tomato (Lycopersicon esculentum Mill.) roots for net NO -3 absorption increased two-to five fold within 2 d of being deprived of NO -3 supply. Nitrogen-starved barley roots continued to maintain a high potential for NO -3 absorption, whereas NO -3 absorption by tomato roots declined below control levels after 10 d of N starvation. When placed in a 0.2 mM NO -3 solution, roots of both species transported more NO -3 and total solutes to the xylem after 2 d of N starvation than did N-sufficient controls. However, replenishment of root NO -3 stores took precedence over NO -3 transport to the xylem. Consequently, as N stress became more severe, transport of NO -3 and total solutes to the xylem declined, relative to controls. Nitrogen stress caused an increase in hydraulic conductance (L p) and exudate volume (J v) in barley but decrased these parameters in tomato. Nitrogen stress had no significant effect upon abscisic acid (ABA) levels in roots of barley or flacca (a low-ABA mutant) tomato, but prevented an agerelated decline in ABA in wild-type tomato roots. Applied ABA had the same effect upon barley and upon the wild type and flacca tomatoes: L p and J v were increased, but NO -3 absorption and NO -3 flux to the xylem were either unaffected or sometimes inhibited. We conclude that ABA is not directly involved in the normal changes in NO -3 absorption and transport that occur with N stress in barley and tomato, because (1) the root ABA level was either unaffected by N stress (barley and flacca tomato) or changed, after the greatest changes in NO -3 absorption and transport and L p had been observed (wild-type tomato); (2) changes in NO -3 absorption/transport characteristics either did not respond to applied ABA, or, if they did, they changed in the direction opposite to that predicted from changes in root ABA with N stress; and (3) the flacca tomato (which produces very little ABA in response to N stress) responded to N stress with very similar changes in NO -3 transport to those observed in the wild type.

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Abbreviations

ABA:

abscisic acid

Jv :

exudate volume

Lp :

root hydraulic conductance

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Authors and Affiliations

  1. Institute of Arctic Biology, University of Alaska, 99775, Fairbanks, AK, USA

    F. Stuart Chapin III

  2. Department of Agricultural Sciences, University of Bristol, Long Ashton Research Station, Long Ashton, BS18 9AF, Bristol, UK

    David T. Clarkson, John R. Lenton & Colin H. S. Walter

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  1. F. Stuart Chapin III
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  2. David T. Clarkson
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  3. John R. Lenton
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  4. Colin H. S. Walter
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Chapin, F.S., Clarkson, D.T., Lenton, J.R. et al. Effect of nitrogen stress and abscisic acid on nitrate absorption and transport in barley and tomato. Planta 173, 340–351 (1988). https://doi.org/10.1007/BF00401021

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