Abstract
The yeast-mycelium dimorphims of the genus Benjaminiella poitrasii has been investigated. To understand the mechanism of dimorphism two stable yeast-phase mutants (Y-1 & Y-2) and one slow growing mycelial mutant (M-1) of B. poitrasii were isolated after NTG treatment of parent strain spores and studied for their biochemical characteristics. Effects of (i) kind and concentration of carbon source, (ii) presence of complex organic nitrogen and (iii) C:N ratio in the growth medium on the morphology of parent and mutant strains were carried out at 28°C under shaking conditions. Ethanol induced morphological change and its reversal were studied in all the strains in order to elucidate the possible mechanism of morphogenesis.
Similar content being viewed by others
References
Bridson FN & Brecker A (1970) Design and formulation of microbial culture media. In: Norris JR & Ribbons DW (Eds) Methods in Microbiology, 3A (pp 229–296) Academic Press, New York
Cannon RD (1986) Isolation of a mycelial mutant of Candida albicans. J. Gen. Microbiol. 132: 2405–2407
Haidlee CW & Storck R (1966) Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem. Biophys. Res. Commun. 22: 174–180.
Inderlied CB & Sypherd PS (1978) Glucose metabolism and dimorphism in Mucor. J. Bacteriol. 133: 1282–1286
Manning M & Mitchell TG (1980) Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain or temperature. J. Bacteriol. 144: 258–273
Martinez-Pacheco M, Rodriguez G, Reyna G, Calvo-Mendez C & Ruiz-Herrera J (1989) Inhibition of the yeast-mycelial transition and the phorogenesis of Mucorales by diamino butanone. Arch. Microbiol. 151: 10–14
Nombela C, Pomes R & Gil C (1987) Protoplasts fusion hybrids from Candida albicans morphological mutants. In: CRC Critical Reviews in Microbiology 15(1): 79–85
Ruiz-Herrera J, Ruiz A & Lopez-Romero E (1983) Isolation and biochemical analysis of Mucor bacilliformis monomorphic mutants. J. Bacteriol. 156: 264–272
San-Blas G & San-Blas F (1983) Molecular aspects of fungal dimorphism. In: CRC Critical Reviews in Microbiology 11 (2): 101–127
Sevilla MJ, Landajuela L & Uruburu F (1983) The effect of alcohols on the morphology of Aureobasidium pullulans. Curr. Microbiol. 9: 169–172
Stewart E, Hawser S & Gow NAR (1989) Changes in internal and external pH accompanying growth of Candida albicans: studies of non-dimorphic variants. Arch. Microbiol. 151: 149–153
Sypherd PS, Borgia PT & Paznokos JL (1978) Biochemistry of dimorphism in the fungus Mucor. In: Rose AH & Morris JG (Eds) Advances in Microbial Physiology 18 (pp 67–104), Academic Press, New York
Tani Y, Yamada Y & Kamihara T (1980) Dimorphism in Candida tropicalis pK233: Inositol prevents the morphological change caused by ethanol. In: Stewart GG & Russell I (Eds) Advances in Biotechnology: Current Developments in Yeast Research (pp 459–464) Pergamon Press, New York
Worthington Enzyme Manual (1977) Dekker LA (Ed) Worthington Biochemical Corporation, Freehold, New Jersey, USA
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Khale, A., Srinivasan, M.C., Deshmukh, S.S. et al. Dimorphism of Benjaminiella poitrasii: Isolation and biochemical studies of morphological mutants. Antonie van Leeuwenhoek 57, 37–41 (1990). https://doi.org/10.1007/BF00400334
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00400334