Skip to main content
Log in

Dimorphism of Benjaminiella poitrasii: Isolation and biochemical studies of morphological mutants

  • Article
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

The yeast-mycelium dimorphims of the genus Benjaminiella poitrasii has been investigated. To understand the mechanism of dimorphism two stable yeast-phase mutants (Y-1 & Y-2) and one slow growing mycelial mutant (M-1) of B. poitrasii were isolated after NTG treatment of parent strain spores and studied for their biochemical characteristics. Effects of (i) kind and concentration of carbon source, (ii) presence of complex organic nitrogen and (iii) C:N ratio in the growth medium on the morphology of parent and mutant strains were carried out at 28°C under shaking conditions. Ethanol induced morphological change and its reversal were studied in all the strains in order to elucidate the possible mechanism of morphogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bridson FN & Brecker A (1970) Design and formulation of microbial culture media. In: Norris JR & Ribbons DW (Eds) Methods in Microbiology, 3A (pp 229–296) Academic Press, New York

    Google Scholar 

  • Cannon RD (1986) Isolation of a mycelial mutant of Candida albicans. J. Gen. Microbiol. 132: 2405–2407

    Google Scholar 

  • Haidlee CW & Storck R (1966) Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem. Biophys. Res. Commun. 22: 174–180.

    Google Scholar 

  • Inderlied CB & Sypherd PS (1978) Glucose metabolism and dimorphism in Mucor. J. Bacteriol. 133: 1282–1286

    Google Scholar 

  • Manning M & Mitchell TG (1980) Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain or temperature. J. Bacteriol. 144: 258–273

    Google Scholar 

  • Martinez-Pacheco M, Rodriguez G, Reyna G, Calvo-Mendez C & Ruiz-Herrera J (1989) Inhibition of the yeast-mycelial transition and the phorogenesis of Mucorales by diamino butanone. Arch. Microbiol. 151: 10–14

    Google Scholar 

  • Nombela C, Pomes R & Gil C (1987) Protoplasts fusion hybrids from Candida albicans morphological mutants. In: CRC Critical Reviews in Microbiology 15(1): 79–85

  • Ruiz-Herrera J, Ruiz A & Lopez-Romero E (1983) Isolation and biochemical analysis of Mucor bacilliformis monomorphic mutants. J. Bacteriol. 156: 264–272

    Google Scholar 

  • San-Blas G & San-Blas F (1983) Molecular aspects of fungal dimorphism. In: CRC Critical Reviews in Microbiology 11 (2): 101–127

  • Sevilla MJ, Landajuela L & Uruburu F (1983) The effect of alcohols on the morphology of Aureobasidium pullulans. Curr. Microbiol. 9: 169–172

    Google Scholar 

  • Stewart E, Hawser S & Gow NAR (1989) Changes in internal and external pH accompanying growth of Candida albicans: studies of non-dimorphic variants. Arch. Microbiol. 151: 149–153

    Google Scholar 

  • Sypherd PS, Borgia PT & Paznokos JL (1978) Biochemistry of dimorphism in the fungus Mucor. In: Rose AH & Morris JG (Eds) Advances in Microbial Physiology 18 (pp 67–104), Academic Press, New York

    Google Scholar 

  • Tani Y, Yamada Y & Kamihara T (1980) Dimorphism in Candida tropicalis pK233: Inositol prevents the morphological change caused by ethanol. In: Stewart GG & Russell I (Eds) Advances in Biotechnology: Current Developments in Yeast Research (pp 459–464) Pergamon Press, New York

    Google Scholar 

  • Worthington Enzyme Manual (1977) Dekker LA (Ed) Worthington Biochemical Corporation, Freehold, New Jersey, USA

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khale, A., Srinivasan, M.C., Deshmukh, S.S. et al. Dimorphism of Benjaminiella poitrasii: Isolation and biochemical studies of morphological mutants. Antonie van Leeuwenhoek 57, 37–41 (1990). https://doi.org/10.1007/BF00400334

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00400334

Key words

Navigation