Abstract
Soybean (Glycine max (L.) Merr.) protoplasts have been surface-labelled with cationized ferritin, and the fate of the label has been followed ultrastructurally. Endocytosis of the label occurs via the coated-membrane system. The pathway followed by the label, once it has been taken into the interior of the protoplast, appears to be similar to that found during receptor-mediated endocytosis in animal cells. Cationized ferritin is first seen in coated vesicles but rapidly appears in smooth vesicles. Labelled, partially coated vesicles are occasionally observed, indicating that the smooth vesicles may have arisen by the uncoating of coated vesicles. Structures which eventually become labelled with cationized ferritin include multivesicular bodies, dictyosomes, large smooth vesicles, and a system of partially coated reticula.
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Abbreviations
- CF:
-
cationized ferritin
References
Bonnett, H.T. (1969) Cortical cell death during lateral root formation. J. Cell Biol. 40, 144–159
Bonnett, H.T., Newcomb, E.H. (1966) Coated vesicles and other cytoplasmic components of growing root hairs of radish. Protoplasma 62, 59–75
Brown, M.S., Anderson, R.G.W., Goldstein, J.L. (1983) Recycling receptors: the roundtrip itinerary of migrant membrane proteins. Cell 32, 663–667
Burgess, J., Linstead, P.J. (1976) Ultrastructural studies of the binding of concanavalin A to the plasmalemma of higher plant protoplasts. Planta 130, 73–79
Cram, W.J. (1980) Pinocytosis in plants. New Phytol. 84, 1–17
Doohan, M.E., Palevitz, B.A. (1980) Microtubules and coated vesicles in guard cell protoplasts of Allium cepa L. Planta 149, 389–401
Fowke, L.C. (1982) Electron microscopy of protoplasts. In: Plant tissue culture methods, pp. 72–76, Welter, L.R., Constabel, F., eds. Plant Biotechnology Institute, Saskatoon, Sask., Canada
Fowke, L.C., Bech-Hansen, C.W., Gamborg, O.L., Constabel, F. (1975) Electron microscope observations of mitosis and cytokinesis in multinucleate protoplasts of soybean. J. Cell Sci. 18, 491–501
Fowke, L.C., Griffing, L.R., Mersey, B.G., Van der Valk, P. (1983) Protoplasts for studies of the plasma membrane and associated cell organelles. In: Protoplasts 1983 (Lecture Proc., 6th Int. Protoplast Symp., Basel, Switzerland). Experientia Suppl. 46, 101–110
Franke, W.W., Herth, W. (1974) Morphological evidence for de-novo formation of plasma membrane from coated vesicles in exponentially growing cultivated plant cells. Exp. Cell Res. 89, 447–451
Fukunaga, Y., Nagata, T., Takebe, I., Kakehi, T., Matsui, C. (1983) An ultrastructural study of the interaction of liposomes with plant protoplasts. Exp. Cell Res. 144, 181–189
Gamborg, O.L. (1982) Callus and cell culture. In: Plant tissue culture methods, pp. 1–10, Wetter, L.R., Constabel, F., eds. Plant Biotechnology Institute, Saskatoon, Sask., Canada
Geuze, H.J., Slot, J.W., Strous, G.J.A.M., Lodish, H.F., Schwartz, A.L. (1983) Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double label immunoelectron microscopy during receptor-mediated endocytosis. Cell 32, 277–287
Kerk, K.N., Gruber, P.J., Frederick, S.E. (1983) Capacity of Daucus carota protoplasts for endocytosis. (Abstr.) Plant Physiol. 72, Suppl., 177
Lucas, W.J., Franceschi, V.R. (1981) Characean charasome complex and plasmalemma vesicle development. Protoplasma 107, 255–267
Mersey, B.G., Fowke, L.C., Constabel, F., Newcomb, E.H. (1982) Preparation of a coated vesicle-enriched fraction from plant cells. Exp. Cell Res. 141, 459–463
Nakamura, S., Miki-Hiroshige, H. (1982) Coated vesicles and cell plate formation in the microspore mother cell. J. Ultrastruct. Res. 80, 302–311
Newcomb, E.H. (1980) Coated vesicles: their occurrence in different plant cell types. In: Coated vesicles, pp. 55–68, Ockleford, C.D., Whyte, A., eds. Cambridge University Press, Cambridge London New York
Otteson, P.D., Courtoy, P.J., Farquhar, M.G. (1980) Pathways followed by membrane recovered from the surface of plasma cells and myeloma cells. J. Exp. Med. 152, 1–19
Pastan, I.H., Willingham, M.C. (1981) Journey to the center of the cell: role of the receptosome. Science 214, 504–509
Pearse, B.M.F., Bretscher, M.S. (1981) Membrane recycling by coated vesicles. Annu. Rev. Biochem. 50, 85–101
Pesacreta, T.C., Lucas, W.J. (1984) Plasma membrane coat and a coated vesicle-associated reticulum of membranes: their structure and possible interrelationship in Chara coralina. J. Cell Biol. 98, 1537–1545
Power, J.B., Cocking, E.C. (1970) Isolation of leaf protoplasts: macromolecule uptake and growth substance response. J. Exp. Bot. 21, 64–70
Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212
Robertson, J.G., Lyttleton, P. (1982) Coated and smooth vesicles in the biogenesis of cell walls, plasma membranes, infection threads, and peribacteroid membranes in root hairs and nodules of white clover. J. Cell Sci. 58, 63–78
Robinson, D.G., Kristen, U. (1982) Membrane flow via the Golgi apparatus of higher plant cells. Int. Rev. Cytol. 77, 89–127
Ryser, U. (1979) Cotton fiber differentiation: occurrence and distribution of coated and smooth vesicles during primary and secondary wall formation. Protoplasma 98, 223–239
Steinman, R.M., Mellman, I.S., Muller, W.A., Cohn, Z.A. (1983) Endocytosis and the recycling of plasma membrane. J. Cell Biol. 96, 1–27
Straubinger, R.M., Hong, K., Friend, D.S., Papahadjopoulos, D. (1983) Endocytosis of liposomes and intracellular fate of encapsulated molecules: encounter with a lower pH compartment after internalization in coated vesicles. Cell 32, 1069–1079
Suzuki, M., Takebe, I., Kajita, S., Honda, Y., Matsui, C. (1977) Endocytosis of polystyrene spheres by tobacco leaf protoplasts. Exp Cell Res. 105, 127–135
Takebe, I. (1979) Protoplasts as a host cell system for plant viruses. In: Advances in protoplast research, pp. 431–440, Ferenczy, L., Farkas, G.L., eds. Pergamon Press, New York
Tanchak, M.A., Griffing, L.R., Mersey, B.G., Fowke, L.C. (1983) Functions of coated vesicles in plant protoplasts: endocytosis of cationized ferritin and transport of peroxidase. (Abstr.) J. Cell Biol. 97, 177a
Thyberg, J., Nilsson, J., Hellgren, D. (1980) Recirculation of cationized ferritin in cultured mouse peritoneal macrophages. Electron microscopic and cytochemical studies with double-labelling technique. Eur. J. Cell Biol. 23, 85–94
Van der Valk, P., Fowke, L.C. (1981) Ultrastructural aspects of coated vesicles in tobacco protoplasts. Can. J. Bot. 59, 1307–1313
Williamson, F.A., Fowke, L.C., Constabel, F.C., Gamborg, O.L. (1976) Labelling of concanavalin A sites on the plasma membrane of soybean protoplasts. Protoplasma 89, 305–316
Willingham, M.C., Pastan, I. (1983) Formation of receptosomes from plasma membrane coated pits during endocytosis: analysis by serial sections with improved membrane labelling and preservation techniques. PNAS 80, 5617–5621
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Tanchak, M.A., Griffing, L.R., Mersey, B.G. et al. Endocytosis of cationized ferritin by coated vesicles of soybean protoplasts. Planta 162, 481–486 (1984). https://doi.org/10.1007/BF00399912
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DOI: https://doi.org/10.1007/BF00399912