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Surface phenotypes of human peripheral blood mononuclear cells from patients with gastrointestinal carcinoma

  • Original Papers
  • Clinical Oncology or Epidemiology
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Summary

Peripheral blood mononuclear cells (PBMC) from 40 patients with gastrointestinal carcinoma (GIC), 13 patients with primary carcinoma in other localizations(non-GIC), and from 57 apparently healthy donors were isolated by Ficoll-Paque gradient centrifugation. The separated cells were stained with several monoclonal antibodies and subjected to analysis on a fluorescence-activated cell sorter. A decreased percentage of PBMC expressing T cell antigens was noted amongst GIC patients, and was mainly due to a reduction of the Leu 2a subset, thus, leading to an increase in the Leu3a/Leu2a ratio from 1.4 to 2.1. Non-GIC patients had decreased numbers of both T helper and suppressor cells. Amongst PBMC from GIC and non-GIC patients a statistically increased percentage of cells expressed LeuM2 (P<0.001), LeuM3 (P<0.001), OKM 1 (P<0.005), VEP 9 (P<0.001), and HLA-DR (P<0.001) antigens compared to healthy controls. The percentage of cells bearing these monocyte/macrophage antigens correlated well with the number of cells having monocyte morphology, stained for non-specific esterase, phagocytosed latex particles, and expressed Fc IgG receptor. Our results demonstrate clearly that tumor-bearing patients have an incrased relative number of monocytes. The data suggest that cells of the macrophage lineage may be involved in defense mechanisms and changes of the immune system evoked by various tumors.

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References

  • Adams DO, Hall T, Steplewski Z, Koprowski H (1984) Tumors undergoing rejection induced by monoclonal antibodies of the IgG2a isotype contain increased numbers of macrophages activated for a distinctive form of antibody-dependent cytolysis. Proc Natl Acad Sci USA 81:3506–3510

    Google Scholar 

  • Ahnen DJ, Nakane PK, Brown WR (1982) Ultrastructural localization of carcinoembryonic antigen in normal intestine and colon cancer: Abnormal distribution of CEA on the surface of colon cancer cells. Cancer 49:2077–2090

    Google Scholar 

  • Akiyama M, Bean MA, Sadamoto K, Takahashi Y, Brankovan V (1983) Suppression of the responsiveness of lymphocytes from cancer patients triggered by co-culture with autologous tumor-derived cells. J Immunol 131:3085–3090

    Google Scholar 

  • Barlozzari T, Reynolds CW, Herberman RB (1983) In vivo role of natural killer cells: Involvment of large granular lymphocytes in the clearance of tumor cells in anti-asialo GM1-treated rats. J Immunol 131:1024–1027

    Google Scholar 

  • Boltz-Nitulescu G, Förster O (1979) Antigenic differences between alveolar and peritoneal macrophages of the rat. Lack of population-specific determinants. Immunology 38:621–630

    Google Scholar 

  • Boltz-Nitulescu G, Bazin H, Spiegelberg HL (1981) Specificity of Fc receptors for IgG2a, IgG1/IgG2b, and IgE on rat macrophages. J Exp Med 154:374–384

    Google Scholar 

  • Braun DP, Cobleigh MA, Harris JE (1980) Multiple concurrent immunoregulatory defects in cancer patients with depressed PHA-induced lymphocyte DNA synthesis. Clin Immunol Immun-pathol 17:89–101

    Google Scholar 

  • Gooi HC, Thorpe SJ, Hounseel EF, Rumpold H, Kraft D, Förster O, Feizi T (1983) Marker of peripheral blood granulocytes and monocytes of man recognized by two monoclonal antibodies VEP 8 and VEP 9 involves the trisaccharide 3-fucosyl-N-acetyl-lactosamine. Eur J Immunol 13:306–312

    Google Scholar 

  • Grimm EA, Mazumder A, Zhang HZ, Rosenberg SA (1982) Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human periphral blood lymphocytes. J. Exp Med 155:1823–1841

    Google Scholar 

  • Hamilton TA, Fishman M, Crawford G, Look AT (1982) Sensitivity to macrophage-mediated cytostasis is cell cycle dependent. Cell Immunol 69:363–373

    Google Scholar 

  • Herlyn M, Steplewski Z, Herlyn D, Koprowski H (1979) Colorectal carcinoma-specific antigen: Detection by means of monoclonal antibodies. Proc Natl Acad Sci USA 76:1438–1442

    Google Scholar 

  • Koch B, Giedl J, Hermanek P, Kalden JR (1983) Analysis of mononuclear cell infiltrations in tissue sections of colon carcinoma. Immunbiology 165:298–299

    Google Scholar 

  • Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497

    Google Scholar 

  • Lanier LL, Engelman EG, Gatenby P, Babcock GF, Werner NL, Herzenberg LA (1983) Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry. Immunol Rev 74:143–160

    Google Scholar 

  • Lattime EC, Pecoraro GA, Stutman O (1983) The activity of natural cytotoxic cells is augmented by interleukin 2 and interleukin 3. J Exp Med 157:1070–1075

    Google Scholar 

  • Ligthart GJ, Corberand JX, Fournier C, Galanaud E, Hijmans W, Kennes B, Müller-Hermelink HK, Steinmann GC (1984) Admission criteria for immunogerontological studies in man: The Senieur protocol. Mech Ageing Dev 28:47–55

    Google Scholar 

  • Mantovani A, Jerrells TR, Dean JH, Herberman RB (1979) Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer 23:18–27

    Google Scholar 

  • Meltzer MS, Occhionero M, Ruco LP (1982) Macrophage activation for tumor cytotoxicity: Regulatory mechanisms for induction and control of cytotoxic activity. Fed Proc 41:2198–2205

    Google Scholar 

  • Miescher S, Whiteside TL, Carrel S, Fliedner V von (1986) Functional properties of tumor-infiltrating and blood lymphocytes in patients with solid tumors: Effects of tumor cells and their supernatants on proliferative responses of lymphocytes. J Immunol 136:1899–1907

    Google Scholar 

  • Müller DS, Manger B, Zawatzky R, Kirchner H, Kalden JR (1984) Mitogen-induced γ interferon production in peripheral blood lymphocytes from patients with colorectal tumors. Immunobiology 166:494–499

    Google Scholar 

  • Oshimi K, Oshimi Y, Satake M, Mizoguchi H (1985) Natural killer-mediated lysis of normal and malignant target cells, and its regulation by monocytes. J Exp Med 162:472–476

    Google Scholar 

  • Perussia B, Trinchieri G, Jackson A, Warner NL, Faust J, Rumpold H, Kraft D, Lanier LL (1984) The Fc receptor for IgG on human natural killer cells: Phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol 133:180–189

    Google Scholar 

  • Reinherz EL, Kung PC, Goldstein G, Schlossman SF (1979) Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061–4065

    Google Scholar 

  • Rumpold H, Kraft D, Obexer G, Böck G, Gebhart W (1982) A monoclonal antibody against a surface antigen shared by human large granular lymphocytes and granulocytes. J Immunol 129:1458–1464

    Google Scholar 

  • Rumpold H, Kraft D, Obexer G, Radaszkiewicz T, Majdic O, Bettelheim P, Knapp W, Böck G (1983) Phenotypes of human large granular lymphocytes as defined by monoclonal antibodies. Immunobiology 164:51–62

    Google Scholar 

  • Schlossman SF, Reinherz EL (1984) Human T-cell subsets in health and disease. Springer Semin Immunopathol 7:9–18

    Google Scholar 

  • Töttermann TH, Parthenais E, Häyry P, Timonen T, Saksela E (1980) Cytological and functional analysis of inflammatory infiltrates in human malignant tumors. III. Further functional investigations using cultured autochthonous tumor cell lines and freeze-thawed infiltrating inflammatory cells. Cell Immunol 55:219–226

    Google Scholar 

  • Traill KN, Schönitzer D, Jürgens G, Böck G, Pfeilschifter R, Hilchenbach M, Holasek A, Förster O, Wick G (1985) Age-related changes in lymphocyte subset proportions, surface differentiation antigen density and plasma membrane fluidity: Application of the EURAGE Senieur protocol admission criteria. Mech Ageing Dev 33:39–66

    Google Scholar 

  • Vanky F, Klein E (1982) Specificity of auto-tumor cytotoxicity exerted by fresh, activated and propagated T lymphocytes. Int J Cancer 29:547–553

    Google Scholar 

  • Vose BM, Vanky F, Klein E (1977) Human tumour-lymphocyte interaction in vitro. V. Comparison of the reactivity of tumour-infiltrating blood and lymph node lymphocytes with autologous tumour cells. Int J Cancer 20:895–902

    Google Scholar 

  • Vose BM, Moore M (1979) Suppressor cell activity of lymphocytes infiltrating human lung and breast tumors. Int J Cancer 24:579–585

    Google Scholar 

  • Zembala M, Mytar B, Popiela T, Asherson GL (1977) Depressed in vitro peripheral brood lymphocyte response to mitogens in cancer patients: The role of suppressor cells. Int J Cancer 19:605–613

    Google Scholar 

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Authors and Affiliations

  1. Institute of General and Experimental Pathology, University of Vienna, Vienna, Austria

    G. Boltz, C. Holzinger, A. Fellinger, C. Wiltschke & K. Langer

  2. First Department of Gastroenterology and Hepatology, University of Vienna, Vienna, Austria

    E. Penner, S. Bakos, M. Walgram & A. Gangl

  3. Institute of Clinical Chemistry and Laboratory Medicine, University of Vienna, Vienna, Austria

    H. Rumpold

Authors
  1. G. Boltz
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  2. E. Penner
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  3. C. Holzinger
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  4. S. Bakos
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  5. A. Fellinger
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  6. M. Walgram
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  7. C. Wiltschke
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  8. H. Rumpold
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  9. K. Langer
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  10. A. Gangl
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Additional information

This work was supported by Fonds österreichischer Krebsforschungsinstitute

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Boltz, G., Penner, E., Holzinger, C. et al. Surface phenotypes of human peripheral blood mononuclear cells from patients with gastrointestinal carcinoma. J Cancer Res Clin Oncol 113, 291–297 (1987). https://doi.org/10.1007/BF00396388

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  • DOI: https://doi.org/10.1007/BF00396388

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