Summary
3H-serine+14C-indole were administered to tips of sterily-grown pea seedlings and of non-sterile oat coleoptiles. The Try and IAA produced were extracted and purified by paper chromatography, and their 3H/14C ratios were determined. The 3H/14C ratio of IAA was lower than the 3H/14C ratio of Try. However, the same decrease of the IAA 3H/14C ratio was found when 3H, 14C-Try was supplied instead of 3H-serine+14C-indole. This result supports the view that Try is the native IAA precursor and that no significant bypass from indole to IAA exists in the plant material used.
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Abbreviations
- IAA:
-
indole-3-acetic acid
- IPyA:
-
indole-3-pyruvic acid
- Try:
-
tryptophan
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Erdmann, N., Schiewer, U. Tryptophan-dependent indoleacetic-acid biosynthesis from indole, demonstrated by double-labelling experiments. Planta 97, 135–141 (1971). https://doi.org/10.1007/BF00386761
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DOI: https://doi.org/10.1007/BF00386761