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Extracellular magnesium regulates intracellular free Mg2+ in vascular smooth muscle cells

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  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Summary

Regulatory effects of extracellular magnesium ions ([Mg2+]o) on intracellular free ionized magnesium ([Mg2+]i) were exmained in cultured vascular smooth muscle cells (VSMCs) fromrat aorta by digital imaging microscopy using the Mg2+ fluorescent probe, Mag-fura-2. With normal Mg2+(1.2 mM)-containing incubation media, [Mg2+]i in VSMCs was 0.63±0.09 mM. The ratio of [Mg2+]i/[Mg2+]o was 0.52±0.07. Elevation of [Mg2+]o up to 4.8 mM induced consistent increments in [Mg2+]i (to a mean values of 1.63±0.08 mM) in 5 min and lowered the ratio of [Mg2+]i/[Mg2+]o to 0.34±0.02. Our data suggest that [Mg2+]o can regulate [Mg2+]i, which may be related to its effects on intracellular Ca2+ ([Ca2+]i) and tone of VSMCs.

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Authors and Affiliations

  1. Department of Physiology, State University of New York, Health Science Center at Brooklyn, 11203, Brooklyn, New York, USA

    Aimin Zhang, Bella T. Altura & Burton M. Altura

  2. Department of Anatomy and Cell Biology, State University of New York, Health Science Center at Brooklyn, 11203, Brooklyn, New York, USA

    Toni P. -O. Cheng

Authors
  1. Aimin Zhang
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  2. Toni P. -O. Cheng
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  3. Bella T. Altura
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  4. Burton M. Altura
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Zhang, A., Cheng, T.P.O., Altura, B.T. et al. Extracellular magnesium regulates intracellular free Mg2+ in vascular smooth muscle cells. Pflügers Arch 421, 391–393 (1992). https://doi.org/10.1007/BF00374229

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  • DOI: https://doi.org/10.1007/BF00374229

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