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Phenotypic determination of T-lymphocytes responding to chemotactic stimulation from fMLP, IL-8, human IL-10, and epidermal lymphocyte chemotactic factor

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Summary

Human T lymphocytes were collected after they had migrated towards N-formyl-methionyl-leukyl-phenylalanine (fMLP), rIL-8, human IL-10 (hIL-10), and epidermal lymphocyte chemotactic factor (ELCF). They were stained for determination of their phenotype by FACS analysis using anti-CD4, -CD8, -CD18, -CD45R0 and OPD4 antibodies. Human IL-10 increased the percentage of CD8+ T lymphocytes in the migrating cell population by 152% compared with cells migrating towards the medium and decreased the number of CD4+ T lymphocytes by 79%. ELCF increased the number of CD4+ T lymphocytes by 18%, and the number of CD45R0+ T lymphocytes by 52%, while the number of CD8+ T lymphocytes was decreased by 20%. rIL-8 increased the number of CD4+ T lymphocytes and decreased the CD8+ T lymphocytes. The distribution of the different subpopulations of T lymphocytes was not changed significantly by fMLP. The observed changes in the phenotypes did not occur when incubating T lymphocytes with the chemotaxins. Our observations demonstrate that individual chemotactic factors will attract specific subsets of T lymphocytes. They may help to explain the predominance of memory T lymphocytes (CD4R0+, CD4+) in allergic contact dermatitis and certain other skin diseases. They also confirm the results of a recent study, that showed hIL-10 to be selectively chemotactic for CD8+ T lymphocytes.

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Authors and Affiliations

  1. Department of Dermatology, Marselisborg Hospital, DK-8000, Aarhus C, Denmark

    C. O. C. Zachariae, T. Jinquan & K. Thestrup-Pedersenl

  2. Institute of Human Genetics, University of Aarhus, DK-8000, Aarhus C, Denmark

    V. Nielsen & K. Kaltoft

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  1. C. O. C. Zachariae
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  2. T. Jinquan
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  3. V. Nielsen
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  4. K. Kaltoft
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  5. K. Thestrup-Pedersenl
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Zachariae, C.O.C., Jinquan, T., Nielsen, V. et al. Phenotypic determination of T-lymphocytes responding to chemotactic stimulation from fMLP, IL-8, human IL-10, and epidermal lymphocyte chemotactic factor. Arch Dermatol Res 284, 333–338 (1992). https://doi.org/10.1007/BF00372035

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  • DOI: https://doi.org/10.1007/BF00372035

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