Abstract
We have found that precardiac mesoderm extirpated from chicken blastoderm at stage 5 fails to differentiate into beating tissue when cultured in Eagle's minimum essential medium (MEM), while it can pulsate provided either the endoderm is present or serum is added to the MEM. To identify the factor(s) which influence early myocardial differentiation, we examined the effect of insulin-like growth factor 1 (IGF-1), activin A and basic fibroblast growth factor (bFGF). All these growth factors showed a stimulating effect on myocardial differentiation and it is conceivable that these factors exhibit the same effect in vivo.
This is a preview of subscription content, log in via an institution to check access.
References
Arias M, Garcia C, Villar JM (1987) Ultrastructural analysis of chick embryo blastoderms explanted in vitro in absence of endoderm: differentiation capacity of mesoderm. Acta Anat 128: 27–32
Asashima M, Nakano H, Shimada K, Kinoshita K, Ishii K, Shibai H, Ueno N (1990) Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor). Roux's Arch Dev Biol 198: 330–335
De Pablo F, Scott LA, Roth J (1990) Insulin and insulin-like growth factor 1 in early development: peptides, receptors and biological events. Endocr Rev 11: 558–577
Gonzalez-Sanchez A, Bader D (1990) In vitro analysis of cardiac progenitor cell differentiation. Dev Biol 139: 197–209
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Jacobson AG, Sater AK (1988) Features of embryonic induction. Development 104:341–359
Kokan-Moore NP, Bolender DL, Lough J (1991) Secretion of inhibin βA by endoderm cultured from early embryonic chicken. Dev Biol 146: 242–245
Lee PL, Jonson DE, Cousens LS, Fried VA, Williams LT (1989) Purification and complementary DNA cloning of a receptor for basic fibroblast growth factor. Science 245: 57–60
Mathews LS, Vale WW (1991) Expression cloning of an activin receptor, a predicted transmembrane serine kinase. Cell 65:973–982
Mitrani E, Ziv T, Thomsen G, Shimoni Y, Melton DA, Bril A (1990) Activin can induce the formation of axial structures and is expressed in the hypoblast of the chick. Cell 63: 495–501
Muslin AJ, Williams LT (1991) Well-defined growth factors promote cardiac development in axolotl mesodermal explants. Development 112: 1095–1101
Orts-Llorca F (1963) Influence of the endoderm on heart differentiation during the early stages of development of the chicken embryo. Wilhelm Roux Arch Entwicklungsmech Org 154:533–551
Ohuchi H, Noji S, Koyama E, Myokai F, Nishikawa K, Nohno T, Tashiro K, Shiokawa K, Matsuo N, Taniguchi S (1992) Expression pattern of the activin receptor type IIA gene during differentiation of chick neural tissues, muscle and skin. FEBS Lett 303:185–189
Parlow MH, Bolendar DL, Kokan-Moore NP, Lough J (1991) Localization of bFGF-like proteins as punctate inclusions in the preseptation myocardium of the chicken embryo. Dev Biol 146:139–147
Renaud D, Le Douarin G (1968) Influence de l'environment tissulaire et des conditions de culture sur l'évolution du mésoderme précardiaque de l'embryon de Poulet. C R Acad Sci Set D 267:431–434
Stalsberg H, DeHaan RL (1969) The precardiac areas and formation of the tubular heart in the chick embryo. Dev Biol 19: 128–159
Sugi Y, Sasse J, Lough J (1993) Inhibition of precardiac mesoderm cell proliferation by antisense oligodeoxynucleotide complementary to fibroblast growth factor-2 (FGF-2). Dev Biol 157:28–37
Wiens D, Sullins M, Spooner BS (1984) Precardiac mesoderm differentiation in vitro: actin-isotype synthetic transitions, myofibrillogenesis, initiation of heartbeat, and the possible involvement of collagen. Differentiation 28: 62–72
Yamazaki Y, Hirakow R (1991) Factors required for differentiation of chick precardiac mesoderm cultured in vitro. Proc Jpn Acad [Ser. B] 67: 165–169
Author information
Authors and Affiliations
Additional information
Correspondence to: Y. Yamazaki
Rights and permissions
About this article
Cite this article
Yamazaki, Y., Hirakow, R. Effect of growth factors on the differentiation of chick precardiac mesoderm in vitro. Roux's Arch Dev Biol 203, 290–294 (1994). https://doi.org/10.1007/BF00360525
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00360525