Abstract
Metaphase chromosomes of HeLa D98/AH-2 cells were studied by GTG-banding, NOR-silver staining and in situ hybridization using libraries specific for each human chromosome as probes (chromosome painting). The structure and composition of all marker chromosomes could be determined, allowing a critical assessment of earlier studies. The revised HeLa D98/AH-2 karyotype should provide a reference standard in certain cancer cytogenetic studies.
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Bengtsson BO, Nabholz M, Kennett R, Bodmer WF, Povey S, Swallow D (1975) Human intraspecific somatic cell hybrids: a genetic and karyotypic analysis of crosses between lymphocytes and D98/AH-2. Somat Cell Genet 1: 41–64
Claussen U, Kleider WS, Müller HG, Wille N, Baumann HA (1992) Quality control in routine chromosome analysis: prediction of total number of bands for the individual case analysed. Clin Genet 41: 100–104
Cremer T, Lichter P, Borden J, Ward DC, Manuelidis L (1988) Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosomespecific library probes. Hum Genet 80: 235–246
Francke U, Hammond DS, Schneider JA (1973) The band patterns of twelve D98/AH-2 marker chromosomes and their use for identification of intraspecific cell hybrids. Chromosoma 41: 111–121
Gey GO, Coffman WD, Kubicek MT (1952) Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res 12: 264–265
Heneen WK (1976) HeLa cells and their possible contamination of other cell lines: karyotype studies. Hereditas 82: 217–248
Howell WM, Black DA (1978) A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes. Hum Genet 43: 53–56
Howell WM, Black DA (1980) Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014
Klever M, Grond-Ginsbach C, Scherthan H, Schroeder-Kurth TM (1991) Chromosomal in situ suppression hybridization after Giemsa banding. Hum Genet 86: 484–486
Klinger HP (1982) Suppression of tumorigenicity. Cytogenet Cell Genet 32: 68–84
Klinger HP, Kaelbling M (1986) Suppression of tumorigenicity in somatic cell hybrids. IV. Chromosomes of normal human cells associated with suppression of tumorigenicity in hybrids with D98/AH-2 carcinoma cells. Cytogenet Cell Genet 42: 225–235
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80: 224–234
Miller OJ, Miller DA, Allerdice PW, Dev VG, Grewal MS (1971) Quinacrine fluorescent karyotypes of human diploid and heteroploid cell lines. Cytogenetics 10: 338–346
Nelson-Rees WA, Zhdanov VM, Hawthorne PK, Flandermeyer RR (1974) HeLa-like marker chromosomes and type-A variant glucose-6-phosphate dehydrogenase isoenzyme in human cell cultures producing Mason-Pfizer Monkey virus-like particles. J Nat Cancer Inst 53: 751–757
Nelson-Rees WA, Hunter L, Darlington GJ O'Brien SJ (1980) Characteristics of HeLa strains: permanent vs. variable features. Cytogenet Cell Genet 27: 216–231
Pinkel D, Landegent J Collins C, Fuscoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85: 9138–9142
Rosenberg C, Blakemore KJ, Kearns WC, Giraldez RA, Escallon CS, Pearson PL, Stetten G, (1992) Analysis of reciprocal translocations by chromosome painting: applications and limitations of the technique. Am J Hum Genet 50: 700–705
Rueß D (1992) Zytogenetische und molekulare Charakterisierung von tumorigenen und nicht-tumorigenen HeLa x Fibroblasten Zellhybriden. Diplomarbeit, Universität Hohenheim
Stanbridge EJ (1976) Suppression of malignancy in human cells. Nature 260: 17–20
Stanbridge EJ, Flandermeyer RR, Daniels DW, Nelson-Rees WA (1981) Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids. Somat Cell Genet 7: 699–712
Trask B (1991) DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization. Methods Cell Biol 35: 3–35
Zankl H, Bernhardt S (1977) Combined silver staining of the nucleolus organizing regions and Giemsa banding in human chromosomes. Hum Genet 37: 79–80
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Communicated by: D. Schweizer
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Rueß, D., Ye, LZ. & Grond-Ginsbach, C. HeLa D98/AH-2 studied by chromosome painting and conventional cytogenetical techniques. Chromosoma 102, 473–477 (1993). https://doi.org/10.1007/BF00357102
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DOI: https://doi.org/10.1007/BF00357102