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The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15

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Abstract

Type II collagen is the major extracellular matrix component of cartilage and correct expression of the α1(II) collagen gene is important for vertebrate skeletal development. In order to provide the basis for studying the control of type II collagen gene expression in embryogenesis and in mouse models of human connective tissue disease, the complete mouse Col2-a1 gene has been isolated in a single cosmid clone, cosMcol.2, and partially characterized. The gene is approximately 30 kb and is highly conserved in exon/intron structure and nucleotide and amino acid sequence (>80% homology) when compared with the human, rat, bovine and chicken equivalents. A high degree of conservation was also found in the 5′ flanking region of the rat, human and mouse α1(II) collagen genes, including the presence of several G+C and C+T rich, direct repeat motifs. The sites of transcription start, termination codon and polyadenylation have also been identified. Unlike chicken, bovine and human, where polyA attachment is at a single site, for the mouse Col2a-1 gene two polyadenylation sites are utilized. Col2a-1 has also been localized by interspecies backcross analysis to the central portion of mouse Chromosome (Chr) 15, approximately 8 centiMorgans (cM) proximal of Int-1 and 18 cM distal of Myc. Col2a-1 is therefore included in a linkage group which is conserved on human Chr 12q.

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Authors and Affiliations

  1. Department of Biochemistry, Hong Kong University, Sassoon Road, Hong Kong

    Kathryn S. E. Cheah, Patrick K. C. Au & Elizabeth T. Lau

  2. Department of Biochemistry, Imperial College of Science, Technology and Medicine, SW7 2AY, London, UK

    Peter F. R. Little

  3. Lincoln's Inn Fields, Imperial Cancer Research Fund, WC2A 3PX, London, UK

    Lisa Stubbs

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  1. Kathryn S. E. Cheah
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  2. Patrick K. C. Au
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  3. Elizabeth T. Lau
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  4. Peter F. R. Little
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  5. Lisa Stubbs
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The sequence data presented in this paper have been submitted to GenBank and have been assigned the accession numbers M63708, M63709 and M63710.

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Cheah, K.S.E., Au, P.K.C., Lau, E.T. et al. The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15. Mammalian Genome 1, 171–183 (1991). https://doi.org/10.1007/BF00351064

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