Summary
Plasmid pPGH1 originating from Pseudomonas putida strain H carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. Besides mobilization of pPGH1 by a plasmid of the incompatibility group P-1, hybrid plasmids conferring the Phl+ phenotype could be selected, when R68.45 was the conjugative plasmid. The hybrids contain the complete R68.45 and part of pPGH1. Integration of Phl-DNA of pPGH1 into R68.45 occurred exclusively via the IS21 region of R68.45.
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Communicated by W. Goebel
Dedicated to Udo Taubeneck on the occasion of his 60th birthday
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Herrmann, H., Janke, D., Krejsa, S. et al. In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype. Mol Gen Genet 214, 173–176 (1988). https://doi.org/10.1007/BF00340199
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DOI: https://doi.org/10.1007/BF00340199