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Cold sensitivity in the transfer of a plasmid with a deletion hot spot into recombination deficient B. subtilis cells

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Summary

Various recombination-deficient mutants of B. subtilis, which are readily transformable by plasmid DNA at 42° C cannot be transformed at 30° C with chimeric plasmid derivatives that contain the deletion hot spot defined previously (Alonso and Trautner 1985a, b). Such interference was also observed in protoplast transformation and SPP1 transduction.

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References

  • Alonso JC, Trautner TA (1984) A mutation within plasmid pC194 affecting its transfer into recipient cells. Proceedings of the 7th European Meeting on Genetic Transformation, Paris, pp 114–120

  • Alonso JC, Trautner TA (1985a) A gene controlling segregation of the Bacillus subtilis plasmid pC194. Mol Gen Genet 198:427–431

    Google Scholar 

  • Alonso JC, Trautner TA (1985b) Generation of deletions through a cis acting mutation in plasmid pC194. Mol Gen Genet 198:432–436

    Google Scholar 

  • Canosi U, Morelli G, Trautner TA (1978) The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Mol Gen Genet 166:259–267

    Google Scholar 

  • Canosi U, Iglesias A, Trautner TA (1981) Plasmid transformation in Bacillus subtilis: Effects of insertion of Bacillus subtilis DNA into plasmid pC194. Mol Gen Genet 181:434–440

    Google Scholar 

  • Canosi U, Lüder G, Trautner TA (1982a) SPP1 mediated plasmid transduction. J Virol 44:431–436

    Google Scholar 

  • Canosi U, Mazza G, Tailor R (1982b) Identification of a mutation in a Bacillus subtilis plasmid affecting host sensitivity to methyl methane sulfonate Genetic exchange: A celebration and a new generation. Genetic and cellular technology, vol 1. In: Streips UN, Goodgal SH, Guild WR, Wilson GA (eds). Marcel Dekker Inc, New York and Basel, pp 107–116

    Google Scholar 

  • Chang ACY, Cohen SN (1979) High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet 168:111–115

    Google Scholar 

  • Ehrlich SD (1978) DNA cloning in Bacillus subtilis. Proc Natl Acad Sci USA 75:1433–1436

    Google Scholar 

  • Gryczan TJ, Contente S, Dubnau D (1978) Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bact 134:318–329

    Google Scholar 

  • Iordanescu S (1975) Recombinant plasmid obtained from two different, compatible staphylococcal plasmids. J Bact 124: 597–601

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harb Laboratory

  • Mazza G, Fortunato A, Ferrari E, Canosi U, Falaschi A, Polsinelli M (1975) Genetic and enzymic studies on the recombination process in Bacillus subtilis. Mol Gen Genet 136:9–30

    Google Scholar 

  • Mazza G, Galizzi A (1978) The genetics of DNA replication, repair and recombination in Bacillus subtilis. Microbiologica 1:111–135

    Google Scholar 

  • Rottländer E, Trautner TA (1970) Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol Gen Genet 108:47–60

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für molekulare Genetik, Ihnestrasse 63/73, D-1000, Berlin 33

    Juan Carlos Alonso & Thomas A. Trautner

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  1. Juan Carlos Alonso
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  2. Thomas A. Trautner
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Communicated by H. Saedler

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Alonso, J.C., Trautner, T.A. Cold sensitivity in the transfer of a plasmid with a deletion hot spot into recombination deficient B. subtilis cells. Mol Gen Genet 198, 437–440 (1985). https://doi.org/10.1007/BF00332936

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  • DOI: https://doi.org/10.1007/BF00332936

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