Summary
When plasmid pC194-1 is ligated to pBR322 to generate plasmid pHV15-1, deletions occur with high frequency within the joined pBR322 DNA. Generation of deletions is recE4 independent, and occurs in B. subtilis with a 1,000-fold higher frequency than in Escherichia coli. In the hybrid plasmid pVH15-1, deletion end-points are not at random, but at defined locations within pBR322. We propose that the base alteration, characterizing pC194-1, has stabilized within the plasmid a stem/loop structure, which acts as a deletion generator.
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Communicated by H. Saedler
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Alonso, J.C., Trautner, T.A. Generation of deletions through a cis-acting mutation in plasmid pC194. Mol Gen Genet 198, 432–436 (1985). https://doi.org/10.1007/BF00332935
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DOI: https://doi.org/10.1007/BF00332935