Summary
A peculiar type of cell, the ECL cell, accounts for a large portion of nonenterochromaffin endocrine cells in the gastric oxyntic glands of several mammals, including rat, mouse, guinea pig, rabbit, cat, dog, pig, and man. The ECL cell is characterized mainly by its secretory granules with irregular cores heavily reacting to Grimelius' silver, Sevier-Munger silver and phosphotungstic acid, while failing to react, in all species but the cat and rabbit, to Masson's argentaffin method. In the rat and mouse, the ECL cell seems to correspond exactly to the argyrophil non-argentaffin histamine-storing “enterochromaffin-like” cell of Håkanson and Owman (1967); in the remaining species, ECL cells seem to account for only a part of the gastric argyrophil “enterochromaffin-like” cells described by Håkanson and coworkers. Besides sporadic amines, ECL cell granules store non-amine components, whose possible nature is discussed.
Similar content being viewed by others
References
Black, W. C., Haffner, H. E.: Diffuse hyperplasia of gastric argyrophil cells and multiple carcinoid tumors. Cancer (Philad.) 21, 1080–1099 (1968).
Bussolati, G., Capella, C., Vassallo, G., Solcia, E.: Histochemical and ultrastructural studies on pancreatic A cells. Evidence for glucagon and non-glucagon components of the a granule. Diabetologia (1971, in press).
Capella, C., Solcia, E., Vassallo, G.: Identification of six types of endocrine cells in the gastrointestinal mucosa of the rabbit. Arch. histol. japon. 30, 479–495 (1969).
Carvalheira, A. F., Welsch, U., Pearse, A. G. E.: Cytochemical and ultrastructural observations on the argentaffin and argyrophil cells of the gastro-intestinal tract in mammals, and their place in the APUD series of polypeptide-secreting cells. Histochemie 14, 33–46 (1968).
Cavallero, C., Solcia, E., Vassallo, G., Capella, C.: Cellule endocrine della mucosa gastroenterica e loro possibile funzione. Rend. R. Gastroent. 1, 51–61 (1969).
Forssmann, W. G., Orci, L., Pictet, R., Renold, A. R., Rouiller, C.: The endocrine cells in the epithelium of the gastrointestinal mucosa of the rat. An electron microscope study. J. Cell Biol. 40, 692–715 (1969).
Grimelius, L.: A silver nitrate stain for α2 cells in human pancreatic islets. Acta Soc. Med. upsalien. 73, 243–270 (1968).
Håkanson, R., Liedberg, G., Lindstrand, K.: Endocrine cells in mammalian gastric mucosa: possible storage sites for vitamine B12-binding proteins. Experientia (Basel) 26, 357–359 (1970).
—, Lilja, B., Owman, Ch.: Properties of a new system of amine-storing cells in the gastric mucosa of the rat. Europ. J. Pharmacol. 1, 188–199 (1967).
—, Owman: Concomitant histochemical demonstration of histamine and catecholamines in enterochromaffin-like cells of gastric mucosa. Life Sci. 6, 759–766 (1967).
—: Argyrophilic reaction of histamine-containing epithelial cells in murine gastric mucosa. Experientia (Basel) 25, 525–526 (1969).
—, Sjöberg, N.-O., Sporrong, B.: Amine mechanisms in enterochromaffin and enterochromaffin-like cells of the gastric mucosa in various mammals. Histochemie 21, 189–220 (1970a).
Hosoda, S., Nakamura, W., Snell, K. C., Stewart, H. L.: Histamine production by transplantable argyrophilic gastric carcinoid of Praomys (Mastomys) natalensis. Science 170, 454–455 (1970).
Noll, W. W., Levine, R. J.: Histidine decarboxylase in gastric tissues of primates. Biochem. Pharmacol. 19, 1043–1053 (1970).
Orci, L., Pictet, R., Forssmann, W. G., Renold, A. E., Rouiller, Ch.: Structural evidence of glucagon producing cells in the intestinal mucosa of the rat. Diabetologia 4, 56–57 (1968).
Pearse, A. G. E.: The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologie physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969).
—, Coulling, I., Weavers, B., Friesen, S.: The endocrine polypeptide cells of the human stomach, duodenum, and jejunum. Gut 11, 649–658 (1970).
Rubin, W.: Proliferation of endocrine-like (enterochromaffin) cells in atrophie gastric mucosa. Gastroenterology 57, 641–648 (1969).
Sevier, A. C., Munger, B. L.: A silver method for paraffin sections of neural tissue. J. Neuropath. exp. Neurol. 24, 130–135 (1965).
Silverman, L., Glick, D.: The reactivity and staining of tissue proteins with phosphotungstic acid. J. Cell Biol. 40, 761–767 (1969).
Singh, I.: A modification of the Masson-Hamperl method for staining of argentaffin cells. Anat. Anz. 115, 81–82 (1964).
Solcia, E., Capella, C., Vassallo, G.: Lead-haematoxylin as a stain of endocrine cells. Significance of staining and comparison with other selective methods. Histochemie 20, 116–126 (1969).
—: Endocrine cells of the stomach and pancreas in states of gastric hypersecretion. Rend. R. Gastroent. 2, 147–158 (1970).
—, Sampietro, R., Capella, C.: Differential staining of catecholamines, 5-hydroxytryptamine and related compounds in aldehyde-fixed tissues. Histochemie 17, 273–283 (1969a).
—, Vassallo, G., Capella, C.: Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol. 43, 257–263 (1968).
—: Cytology and cytochemistry of hormone producing cells of the upper gastrointestinal tract. In: Origin, Chemistry, Physiology and Pathophysiology of the Gastrointestinal Hormones (W. Creutzfeldt, ed.). p. 3 Stuttgart: Schattauer 1970a.
—, Sampietro, R.: Endocrine cells in the antro-pyloric mucosa of the stomach. Z. Zellforsch. 81, 474–486 (1967).
Vassallo, G., Capella, C., Solcia, E.: Grimelius' silver stain for endocrine cell granules, as shown by electron microscopy. Stain Technol. 46, 7–13 (1971).
—: Endocrine cells of the human gastric mucosa. Z. Zellforsch. 118, 49–67 (1971a).
Vassallo, G., Solcia, E., Capella, C.: Light and electron microscopic identification of several types of endocrine cells in the gastrointestinal mucosa of the cat. Z. Zellforsch. 98, 333–356 (1969).
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965).
Williams, E. D., Sandler, M.: The classification of carcinoid tumours. Lancet 1, 238–239 (1963).
Author information
Authors and Affiliations
Additional information
This investigation was supported in part by Farmitalia S.p.A., Milano.
Rights and permissions
About this article
Cite this article
Capella, C., Vassallo, G. & Solcia, E. Light and electron microscopic identification of the histamine-storing argyrophil (ECL) cell in murine stomach and of its equivalent in other mammals. Z. Zellforsch. 118, 68–84 (1971). https://doi.org/10.1007/BF00331767
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331767