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Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ

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Summary

The nucleotide sequence of the DNA encoding the traM, finP and the promoter proximal segment of the traJ gene of the F plasmid has been determined. The predicted amino acid sequence for the traM protein shows that this inner membrane protein contains no signal sequence. The promoters for both the traM and traJ genes have been mapped by in vitro transcription and nuclease SI protection experiments. No unambiguous location can be assigned to the finP gene but all candidates, if translated, would encode small proteins of between 24 and 52 amino acids.

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References

  • Achtman M (1975) Mating aggregates in Escherichia coli conjugation. J Bacteriol 123:505–515

    Google Scholar 

  • Achtman M, Manning PA, Edelbluth C, Herrlich P (1979) Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells. Proc Natl Acad Sci USA 76:4837–4841

    Google Scholar 

  • Achtman M, Skurray RA, Thompson R, Helmuth R, Hall S, Beutin L, Clark AJ (1978) Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. J Bacteriol 133:1383–1392

    Google Scholar 

  • Atkins JF (1979) Is UAA or UGA part of the recoginition signal for ribosomal initiation? Nucleic Acids Res 7:1035–1041

    Google Scholar 

  • Berk AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732

    Google Scholar 

  • Clements JB, Sinsheimer RL (1975) Process of infection with bacteriophage ϕX174 XXXVII RNA metabolism in ϕX174-infected cells. J Virol 15:151–160

    Google Scholar 

  • Everett R, Willetts N (1980) Characterisation of an in vivo system for nicking at the origin of conjugal DNA transfer of the sex factor F. J Mol Biol 136:129–150

    Google Scholar 

  • Finnegan DJ, Willetts N (1973) The site of action of the F transfer inhibitor. Mol Gen Genet 127:307–316

    Google Scholar 

  • Gray CP, Sommer R, Polke C, Beck E, Schaller H (1978) Structure of the origin of DNA replication of bacteriophage fd. Proc Natl Acad Sci USA 75:50–53

    Google Scholar 

  • Helmuth R, Achtman M (1975) Operon structure of DNA transfer cistrons on the F sex factor. Nature 257:652–656

    Google Scholar 

  • Ikemura T (1981) Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J Mol Biol 146:1–21

    Google Scholar 

  • Johnson D, Everett R, Willetts N (1981) Cloning of F DNA fragments carrying the origin of transfer oriT and the fertility inhibition gene finP. J Mol Biol 153:187–202

    Google Scholar 

  • Kassavetis GA, Geiduschek EP (1982) Bacteriophage T4 late promoters: mapping 5′ ends of T4 gene 23 mRNAs. EMBO Journal 1:107–114

    Google Scholar 

  • Kennedy N, Beutin L, Achtman M, Skurray RA, Rahmsdorf U, Herrlich P (1977) Conjugation proteins encoded by the F sex factor. Nature 270:580–585

    Google Scholar 

  • Kingsman AR, Willetts NS (1978) The requirement for conjugal DNA synthesis in the donor strain during Flac transfer. J Mol Biol 121:287–300

    Google Scholar 

  • Kyte JF, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132

    Google Scholar 

  • Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564

    Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. In: Grossman L, Moldave R (eds) Methods in enzymology, vol 65. Academic Press, New York, pp 499–580

    Google Scholar 

  • McIntire S, Willetts N (1980) Transfer-deficient cointegrates of Flac and lambda prophage. Mol Gen Genet 178:165–172

    Google Scholar 

  • Queen C, Rosenberg M (1981) A promoter of pBR322 activated by cAMP receptor protein. Nucleic Acids Res 9:3365–3377

    Google Scholar 

  • Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Ann Rev Genet 13:319–353

    Google Scholar 

  • Shine J, Dalgarno L (1975) Determinant of cistron specificity in bacterial ribosomes. Nature 254:34–38

    Google Scholar 

  • Smith HO, Birnstiel M (1976) A simple method for DNA restriction site mapping. Nucleic Acids Res 3:2387–2398

    Google Scholar 

  • Staden R (1978) Sequence data handling by computer. Nucleic Acids Res 4:4037–4051

    Google Scholar 

  • Staden R (1978) Further procedures for sequence analysis by computer. Nucleic Acids Res 5:1013–1015

    Google Scholar 

  • Steitz JA (1979) Genetic signals and nucleotide sequences in messenger RNA. In: Goldberger RF (ed) Biological regulation and development, vol 1. Gene expression. Plenum Press, New York, pp 349–389

    Google Scholar 

  • Sutcliffe JG (1978) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43:77–90

    Google Scholar 

  • Thompson R, Achtman M (1978) The control region of the F sex factor DNA transfer cistrons: restriction mapping and DNA cloning. Mol Gen Genet 165:295–304

    Google Scholar 

  • Thompson R, Achtman M (1979a) The control region of the F sex factor DNA transfer cistrons: physical mapping by deletion analysis. Mol Gen Genet 169:49–57

    Google Scholar 

  • Thompson R, Achtman M (1979b) Nucleotide sequence of the F DNA transfer origin. In: Glover SW and Butler LO (eds) Proc 4th European Transformation and Transfection Meeting, Cotswold Press, Oxford, pp 331–338

    Google Scholar 

  • Twigg AJ, Sherratt DJ (1980) Trans-complementable copy-number mutants of plasmid ColE1. Nature 283:216–218

    Google Scholar 

  • Weaver RF, Weissman C (1979) Mapping RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15S-globin mRNA precursor and mature 10S-globin mRNA have identical map co-ordinates. Nucleic Acids Res 7:1175–1193

    Google Scholar 

  • Wickner W (1980) Assembly of proteins into membranes. Science 210:861–869

    Google Scholar 

  • Willetts N (1977) The transcriptional control of fertility in F-like plasmids. J Mol Biol 112:141–148

    Google Scholar 

  • Willetts N (1980) Sites and systems for conjugal DNA transfer in bacteria. In: Levy SB, Clowes RC, Koenig EL (eds) Molecular biology pathogenicity and ecology of bacterial plasmids. Plenum Press, New York, pp 207–215

    Google Scholar 

  • Willetts N, Skurray R (1980) The conjugation system of F-like plasmids. Ann Rev Genet 14:41–76

    Google Scholar 

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Communicated by W. Arber

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Thompson, R., Taylor, L. Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ . Mol Gen Genet 188, 513–518 (1982). https://doi.org/10.1007/BF00330058

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  • DOI: https://doi.org/10.1007/BF00330058

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