Summary
An immunohistochemical method is reported using the M-II68 monoclonal antibody, which detects mitochondrial accumulations (“ragged-red fibres”) in routinely processed (formalin-fixed, paraffin-embedded) muscle tissue. Ten cases with electron-microscopically and histochemically proven mitochondrial myopathy featured 4% to 24% ragged-red fibres. In a series of 50 muscle biopsies without mitochondrial myopathy, scattered ragged-red fibres (<0.1%) were present in a few normal and pathological muscles. The immunohistochemical method is specific for mitochondria, does not require frozen tissue and permits rapid examination of large areas.
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Present address and address for offprint requests: Ludwig-Boltzmann-Institut für Klinische Neurobiologie, Krankenhaus Lainz, Wolkersbergenstrasse, A-1130 Wien. Austria
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Paulus, W., Peiffer, J., Roggendorf, W. et al. Ragged-red fibres detected in paraffin sections by a monoclonal antibody to inner mitochondrial membrane. J Neurol 236, 482–483 (1989). https://doi.org/10.1007/BF00328512
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DOI: https://doi.org/10.1007/BF00328512