Abstract
Individual quaking aspen trees vary greatly in foliar chemistry and susceptibility to defoliation by gypsy moths and forest tent caterpillars. To relate performance of these insects to differences in foliar chemistry, we reared larvac from egg hatch to pupation on leaves from different aspen trees and analyzed leaf samples for water, nitrogen, total nonstructural carbohydrates, phenolic glycosides, and condensed tannins. Larval performance varied markedly among trees. Pupal weights of both species were strongly and inversely related to phenolic glycoside concentrations. In addition, gypsy moth performance was positively related to condensed tannin concentrations, whereas forest tent caterpillar pupal weights were positively associated with leaf nitrogen concentrations. A subsequent study with larvae fed aspen leaves supplemented with the phenolic glycoside tremulacin confirmed that the compound reduces larval performance. Larvae exhibited increased stadium durations and decreased relative growth rates and food conversion efficiencies as dietary levels of tremulacin increased. Differences in performance were more pronounced for gypsy moths than for forest tent caterpillars. These results suggest that intraspecific variation in defensive chemistry may strongly mediate interactions between aspen, gypsy moths and forest tent caterpillars in the Great Lakes region, and may account for differential defoliation of aspen by these two insect species.
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References
Arteel GE, Lindroth RL (1992) Effects of aspen phenolic glycosides on gypsy moth (Lepidoptera: Lymantriidae) susceptibility to Bacillus thuringiensis. Great Lakes Entomol 25:239–244
Ayres MP, Suomela J, Maclean SF Jr (1987) Growth performance of Epirrita autumnata (Lepidoptera: Geometridae) on mountain birch: trees, broods, and treexbrood interactions. Oecologia 74:450–457
Batzer HO, Water WE (1956) Forest tent caterpillar (Forest pest leaflet 9). USDA Forest Service, Washington, pp 1–8
Bazzaz FA, Chiariello NR, Coley PD, Pitelka LF (1987) Allocating resources to reproduction and defense. Bioscience 37:58–67
Bryant JP, Clausen TP, Reichardt PB, McCarthy MC, Werner RA (1987) Effect of nitrogen fertilization upon the secondary chemistry and nutritional value of quaking aspen (Populus tremuloides Michx.) leaves for the large aspen tortrix (Choristoneura conflictana (Walker)). Oecologia 73:513–517
Chang KG, Fechner GH, Schroeder HA (1989) Anthocyanins in autumn leaves of quaking aspen in Colorado. For Sci 35:229–236
Chilcote CA, Witter JA, Montgomery ME, Stoyenoff JL (1992) Intra- and inter-clonal variation in gypsy moth larval performance on bigtooth and trembling aspen. Can J For Res 22:1676–1683
Clausen TP, Reichardt PB, Bryant JP, Werner RA (1991) Long-term and short-term induction in quaking aspen: related phenomena? In: Tallamy DW, Raupp MJ (eds) Phytochemical induction by herbivores. Wiley, New York, pp 71–83
Farrar RR Jr, Barbour JD, Kennedy GG (1989) Quantifying food consumption and growth in insects. Ann Entomol Soc Am 82:593–598
Futuyma DJ, Saks ME (1981) The effect of variation in host plant on the growth of an oligophagous insect, Malacosoma americanum and its polyphagous relative, Malacosoma disstria. Ent Exp Appl 30:163–168
Griffin DH, Schaedle M, Manion PD, De Vit M (1991) Clonal variation in amino acid contents of roots, stems, and leaves of aspen (Populus tremuloides Michx.) as influenced by diurnal drought stress. Tree Physiol 8:337–350
Gypsy Moth News (1993) Gypsy moth defoliation 1992. In: Twardus DB (ed) Gypsy moth news 31. USDA Forest Service, Forest Health Protection, Morgantown, West Virginia. p 7
Hagerman AE, Butler LG (1980) Condensed tannin purification and characterization of tannin-associated proteins. J Agric Food Chem 28:947–952
Herbert RB (1989) The biosynthesis of secondary metabolites, 2nd edn. Chapman and Hall, London, pp 96–119
Herms DA, Mattson WJ (1992) The dilemma of plants: to grow or defend. Q Rev Biol 67:284–335
Hodson AC (1941) An ecological study of the forest tent caterpillar, Malacosoma disstria Hubn, in northern Minnesota. Univ Minn Agr Exp Sta Tech Bull 148:1–55
Hwang SY, Lindroth RL, Montgomery ME, Shields KS (1995) Effects of aspen leaf quality on gypsy moth (Lepidoptera: Lymantriidae) susceptibility to Bacillus thuringiensis. J Econ Entomol (in press)
Hunter AF, Lechowicz MJ (1992) Foliage quality changes during canopy development of some northern hardwood trees. Oecologia 89:316–323
Jelinski DE, Fisher LJ (1991) Spatial variability in the nutrient composition of Populus tremuloides: clone-to-clone differences and implications for cervids. Oecologia 88:116–124
Kleiner KW, Montgomery ME (1994) Forest stand susceptibility to the gypsy moth (Lepidoptera: Lymantriidae): species and site effects on foliage quality to larvae. Environ Entomol 23:699–711
Lang CA (1958) Simple microdetermination of Kjeldahl nitrogen in biological materials. Anal Chem 30:1692–1694
Lance DR (1983) Host-seeking behavior of gypsy moth: the influence of polyphagy and highly apparent host plants. In: Ahmad S (ed) Herbivorous insects: host-seeking behavior and mechanisms. Academic Press, New York, pp 201–224
Larsson S, Wirén A, Lundgren L, Ericsson T (1986) Effects of light and nutrient stress on leaf phenolic chemistry in Salix dasyclados and susceptibility to Galerucella lineola (Coleoptera). Oikos 47:205–210
Lechowicz MJ, Mauffette Y (1986) Host preferences of the gypsy moth in eastern North America versus European forests. Rev Entomol Quebec 31:43–51
Lindroth RL, Bloomer MS (1991) Biochemical ecology of the forest tent caterpillar: responses to dietary protein and phenolic glycosides. Oecologia 86:408–413
Lindroth RL, Hemming JDC (1990) Responses of the gypsy moth (Lepidoptera: Lymantriidae) to tremulacin, an aspen phenolic glycoside. Environ Entomol 19:842–847
Lindroth RL, Weisbrod AV (1991) Genetic variation in response of the gypsy moth to aspen phenolic glycosides. Biochem Syst Ecol 19:97–103
Lindroth RL, Hsia MTS, Scriber JM (1987a) Characterization of phenolic glycosides from quaking aspen. Biochem Syst Ecol 15:677–680
Lindroth RL, Hsia MTS, Scriber JM (1987b) Seasonal patterns in the phytochemistry of three Populus species. Biochem Syst Ecol 15:681–686
LIndroth RL, Kinney KK, Platz CL (1993a) Responses of deciduous trees to elevated atmospheric CO2: productivity, phytochemistry and insect performance. Ecology 74:763–777
Lindroth RL, Reich PB, Tjoelker MG, Volin JC, Oleksyn J (1993b) Light environment alters response to ozone stress in seedlings of Acer saccharum Marsh. and hybrid Populus L. III. Consequences for performance of gypsy moth. New Phytol 124:647–651
Mattson WJ, Palmer SR (1988) Changes in levels of foliar minerals and phenolics in trembling aspen, Populus tremuloides, in response to artificial defoliation. In: Mattson WJ, Levieux J, Bernard-Dagan C (eds) Mechanisms of woody plant defenses against insects: search for pattern. Springer, Berlin Heidelberg New York, pp 157–169
Meyer GA, Montgomery ME (1987) Relationships between leaf age and the food quality of cottonwood foliage for the gypsy moth, Lymantria dispar. Oecologia 72:527–532
Montgomery ME (1989) Relationships between foliar chemistry and susceptibility to Lymantria dispar. In: WE Wallner, McManus KA (eds) Lymantriidae: a comparison of features of new and old world tussock moths (General technical report NE-123). USDA Forest Service, Broomall, Pennsylvania, pp 339–350
Nichols-Orians CM, Fritz RS, Clausen TP (1993) The genetic basis for variation in the concentration of phenolic glycosides in Salix sericea: clonal variation and sex-based differences. Biochem Syst Ecol 21:535–542
Palo RT (1984) Distribution of birch (Betula spp.), willow (Salix spp.), and poplar (Populus spp.) secondary metabolites and their potential role as chemical defense against herbivores. J Chem Ecol 10:499–520
Parkinson JA, Allen SE (1975) A wet oxidation procedure suitable for the determination of nitrogen and mineral nutrients in biological material. Commun Soil Sci Plant Anal 6:1–11
Porter LJ, Hrstich LN, Chan BG (1986) The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochem 25:223–230
Robison DJ (1993) The feeding ecology of the forest tent caterpillar, Malacosoma disstria Hubner, among hybrid poplar clones, Populus spp. Ph.D. Thesis, University of Wisconsin, Madison
Roden DB, Surgeoner GA (1991) Survival, development time, and pupal weights of larvae of gypsy moth reared on foliage of common trees of the upper Great Lakes region. North J Appl For 8:126–128
Rossiter MC, Schultz JC, Baldwin IT (1988) Relationships among defoliation red oak phenolics, and gypsy moth growth and reproduction. Ecology 69:267–277
Roth SK, Lindroth RL, Montgomery ME (1994) Effects of foliar phenolics and ascorbic acid on performance of the gypsy moth (Lymantria dispar). Biochem Syst Ecol 22:341–351
SAS Institute (1989) SAS user's guide: statistics, version 6 edn. SAS Institute, Cary
Schultz JC (1983) Habitat selection and foraging tactics of caterpillars in heterogeneous trees. In: Denno RF, McClure MS (eds) Variable plants and herbivores in natural and managed systems. Academic Press, New York, pp 61–90
Suomela J, Nilson A (1994) Within-tree and among-tree variation in growth of Epirrita autumnata on mountain birch leaves. Ecol Entomol 19:45–56
Waldbauer GP (1968) The consumption and utilization of food by insects. Adv Insect Physiol 5:229–288
Waterman PG, Mole S (1994) Analysis of phenolic plant metabolites. Blackwell, London, pp 1–43
Whitham TG (1983) Host manipulation of parasites: within-plant variation as a defense against rapidly evolving pests. In: Denno RF, McClure MS (eds) Variable plants and herbivores in natural and managed systems. Academic Press, New York, pp 61–90
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Hemming, J.D.C., Lindroth, R.L. Intraspecific variation in aspen phytochemistry: effects on performance of gypsy moths and forest tent caterpillars. Oecologia 103, 79–88 (1995). https://doi.org/10.1007/BF00328428
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DOI: https://doi.org/10.1007/BF00328428