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The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses

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Abstract

Chinese hamster ovary cells (line CHO) have been used extensively for metabolic, genetic, and radiobiological studies with only a superficial appreciation for the degree of aneuploidy characteristic of the line. A thorough karyologic analysis of CHO chromosomes using autoradiographic replication patterns, as well as centromere band (C-band) and Giemsa band (G-band) analysis, is presented. Our results demonstrate that only 8 of the 21 CHO chromosomes are normal when compared with euploid Chinese hamster chromosomes. In the 13 altered chromosomes, we found evidence of translocations, deletions, and pericentric inversions. These altered chromosomes have been characterized with respect to both origin and destination of translocated material. With the exception of the X2 chromosome, essentially all of the euploid chromatin is present in CHO cells. Autoradiographic replication patterns show that the normal sequence of chromosomal DNA synthesis is altered. Some sites which replicate late in euploid cells replicate early in CHO, and several late-replicating chromosomes in CHO cells replicate in early- or mid-S in euploid material. These studies may serve to elucidate the observed differences in mutagenic behavior between euploid fibroblasts and CHO cells.

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References

  • Arrighi, F. E., Hsu, T. C.: Localization of heteroohromatin in human chromosomes. Cytogenetics 10, 81–86 (1971).

    Google Scholar 

  • Bianchi, N. O., Ayres, J.: Heterochromatin location on the chromosomes of normal and transformed cells from the African green monkey (Cercopithecus aethiops). DNA denaturation-renaturation method. Exp. Cell Res. 68, 253–258 (1971).

    Google Scholar 

  • Drets, M. E., Shaw, M. W.: Specific banding patterns of human chromosomes. Proc. nat. Acad. Sci. (Wash.) 68, 2073–2077 (1971).

    Google Scholar 

  • Enger, M. D., Tobey, R. A.: Effects of isoleueine deficiency on nucleic acid and protein metabolism in cultured Chinese hamster cells. Continued ribonucleic acid and protein synthesis in the absence of deoxyribonucleic acid synthesis. Biochemistry 11, 269–277 (1972).

    Google Scholar 

  • Gurley, L. R., Hardin, J. M.: The metabolism of histone fractions. I. Synthesis of histone fractions during the life cycle of mammalian cells. Arch. Biochem. Biophys. 128, 285–292 (1968).

    Google Scholar 

  • Hsu, T. C.: Chromosomal evolution in cell populations. Int. Rev. Cytol. 12, 69–161 (1961).

    Google Scholar 

  • Hsu, T. C.: Mammalian chromosomes in vitro. XVIII. DNA replication sequence in the Chinese hamster. J. Cell Biol. 23, 53–62 (1964).

    Google Scholar 

  • Hsu, T. C., Arrighi, F. E.: Distribution of constitutive heterochromatin in mammalian chromosomes. Chromosoma (Berl.) 34, 243–253 (1971).

    Google Scholar 

  • Hsu, T. C., Zenzes, M. T.: Mammalian chromosomes in vitro. XVII. Idiogram of the Chinese hamster. J. nat. Cancer Inst. 32, 857–870 (1964).

    Google Scholar 

  • Kao, F., Puck, T. T.: Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents. J. cell. Physiol. 74, 245–257 (1969).

    Google Scholar 

  • Kao, F., Puck, T. T.: Genetics of somatic mammalian cells: Linkage studies with human-Chinese hamster cell hybrids. Nature (Lond.) 228, 329–332 (1970).

    Google Scholar 

  • Kato, H., Yosida, T. H.: Banding patterns of Chinese hamster chromosomes revealed by new techniques. Chromosoma (Berl.) 36, 272–280 (1972).

    Google Scholar 

  • Kraemer, P. M., Deaven, L. L., Crissman, H. A., Dilla, M. A. van: DNA constancy despite variability in chromosome number. In: Advances in cell and molecular biology (E. J. Du Praw, ed.), vol.II, p. 47–108. New York: Academic Press 1972.

    Google Scholar 

  • Kraemer, P. M., Petersen, D. F., Dilla, M. A. van: DNA constancy in heteroploidy and the stem line theory of tumors. Science 174, 714–717 (1971).

    Google Scholar 

  • Mace, M. L., Tevethia, S.S., Brinkley, B. R.: Differential immunofluorescent labeling of chromosomes with antisera specific for single strand DNA. Exp. Cell Res. (submitted 1972).

  • Marushige, K., Bonner, J.: Fractionation of liver protein. Proc. nat. Acad. Sci. (Wash.) 68, 2941–2944 (1971).

    Google Scholar 

  • Ord, M. G., Stocken, L. A.: Metabolic properties of histones from rat liver and thymus gland. Biochem. J. 98, 888–897 (1966).

    Google Scholar 

  • Pardue, M. L., Gall, J. G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970).

    Google Scholar 

  • Petersen, D. F., Tobey, R. A., Anderson, E. C.: Synchronously dividing mammalian cells. Fed. Proe. 28, 1771–1779 (1969).

    Google Scholar 

  • Puck, T. T., Cieciura, S. J., Robinson, A.: Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J. exp. Med. 108, 945–956 (1958).

    Google Scholar 

  • Ris, H., Kubai, D. F.: Chromosome structure. Ann. Rev. Genet. 4, 263–294 (1970).

    Google Scholar 

  • Seabright, M.: A rapid banding technique for human chromosomes. Lancet II, 971–972 (1971).

    Google Scholar 

  • Schnedl, W.: Analysis of the human karyotype using a reassociation technique. Chromosoma (Berl.) 34, 448–454 (1971).

    Google Scholar 

  • Stubblefield, E.: Mammalian chromosomes in vitro. XIX. Chromosomes of Don C, a Chinese hamster fibroblast strain with a part of autosome Ib translocated to the Y chromosome. J. nat. Cancer Inst. 37, 799–817 (1966).

    Google Scholar 

  • Walters, R. A., Petersen, D. F.: Radiosensitivity of mammalian cells. II. Radiation effects on macromolecular synthesis. Biophys. J. 8, 1487–1504 (1968).

    Google Scholar 

  • Zakharov, A. F., Egolina, N. A.: Asynchrony of DNA replication and mitotic spiralization along heterochromatic portions of Chinese hamster chromosomes. Chromosoma (Berl.) 23, 365–385 (1968).

    Google Scholar 

  • Zakharov, A. F., Egolina, N. A., Kakpakova, E. S.: Late-replicating chromosomal segments in aneuploid Chinese hamster cell lines as determined by autoradiography. J. nat. Cancer Inst. 36, 215–232 (1966).

    Google Scholar 

  • Zakharov, A. F., Kakpakova, E. S., Egolina, N. A., Pogosianz, H. E.: Chromosomal variability in clonal populations of a Chinese hamster cell strain. J. nat. Cancer Inst. 33, 935–956 (1964).

    Google Scholar 

  • Zapisek, W. F., Saponara, A. G., Enger, M. D.: Low molecular weight methylated ribonucleic acid species from Chinese hamster ovary cells. I. Isolation and characterization. Biochemistry 8, 1170–1181 (1969).

    Google Scholar 

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Deaven, L.L., Petersen, D.F. The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses. Chromosoma 41, 129–144 (1973). https://doi.org/10.1007/BF00319690

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