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Molecular cloning and characterization of two distinct hsp 85 sequences from the steroid responsive fungus Achlya ambisexualis

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Summary

In Achlya ambisexualis, hsp85 is one of the characteristic mycelial heat shock proteins induced in response to a rapid elevation in temperature (Silver et al. 1983). This heat shock protein has the same electrophoretic mobility on two-dimensional gels and is antigenically related to an 85 kDa steroid hormone-regulated protein which constites a component of the putative Achlya steroid hormone-receptor complex. We report here the isolation of two distinct, yet highly related, hsp85 gene sequences from Achlya genomic libraries. Northern analyses, using these two Achlya genomic sequences as probes, suggest that there are two hsp85 message population in Achlya and that at least one of these is regulated by the steroid hormone antheridiol.

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References

  • Arsenault GP, Diemann K, Barksdale AW, McMorris TC (1968) J Am Chem Soc 90: 5635–5636

    Google Scholar 

  • Barksdale AW (1969) Science 166: 831–837

    Google Scholar 

  • Barksdale AW, Lasure LL (1973) Bull Torrey Bot Club 100: 199–202

    Google Scholar 

  • Barnier JV, Bensaude O, Morange M, Babinet C (1987) Exp Cell Res 170: 186–194

    Google Scholar 

  • Bedard P-A, Brandhorst BP (1986) Dev Biol 117: 286–293

    Google Scholar 

  • Borkovich KA, Farrelly FW, Finkelstein DB, Taulien J, Linquist S (1989) Mol Cell Biol 9: 3919–3930

    Google Scholar 

  • Brunt SA, Silver JC (1986a) Exp Cell Res 163: 22–34

    Google Scholar 

  • Brunt SA, Silver JC (1986b) Exp Cell Res 165: 306–319

    Google Scholar 

  • Brunt SA, Silver JC (1987) Exp Mycol 11: 65–69

    Google Scholar 

  • Brunt SA, Riehl S, Silver JC (1990) Mol Cell Biol 10: 273–281

    Google Scholar 

  • Catelli GC, Binhart N, Feramisco JR, Helfman DM (1985) Nucleic Acids Res 13: 6036–6047

    Google Scholar 

  • Dodd JG, Horgen PA, Straus NA (1975) Cytobios 13: 31–36

    Google Scholar 

  • Farrelly FW, Finkelstein DB (1984) J Biol Chem 259: 5745–5751

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) Anal Biochem 132: 6–13

    Google Scholar 

  • Groner B, Hynes N, Sippel AE, Schutz G (1976) Nature 261: 599–601

    Google Scholar 

  • Gwynne DI, Brandhorst BP (1980) Exp Mycol 4: 251–259

    Google Scholar 

  • Haire RN, Peterson MS, O'Leary JJ (1988) J Cell Biol 106: 883–891

    Google Scholar 

  • Hickey E, Brandon SE, Sadis S, Smale G, Weber LA (1986) Gene 43: 147–154

    Google Scholar 

  • Hickey E, Brandon SE, Smale G, Lloyd D, Weber LA (1989) Mol Cell Biol 9: 2615–2626

    Google Scholar 

  • Holmgren R, Livak K, Morimoto R, Freund R, Messelson M (1979) Cell 18: 1359–1370

    Google Scholar 

  • Holmgren R, Corses V, Morimoto R, Blackman R, Messelson M (1981) Proc Natl Acad Sci USA 78: 3775–3778

    Google Scholar 

  • Horgen PA (1981) In: O'Day DH, Horgen PA (eds) Sexual interactions in eukaryotic microbes. Academic Press Inc, New York, pp 155–178

    Google Scholar 

  • Horton JS, Horgen PA (1985) Can J Biochem Cell Biol 63: 355–365

    Google Scholar 

  • Lees-Miller SP, Anderson CW (1989) J Biol Chem 264: 2431–2437

    Google Scholar 

  • Linquist S, Craig EA (1988) Annu Rev Genet 22: 631–677

    Google Scholar 

  • Michalski CJ (1978) Biochem Biophys Res Commun 84: 417–427

    Google Scholar 

  • Moore SK, Kozak C, Robinson EA, Ullrich SJ, Appella E (1989) J Biol Chem 264: 5343–5351

    Google Scholar 

  • Mottram JC, Murphy WJ, Agabian N (1989) Mol Biochem Parasitol 37: 115–128

    Google Scholar 

  • Ng R, Abelson J (1980) Proc Natl Acad Sci USA 77: 3912–3916

    Google Scholar 

  • Pekkala D, Silver JC (1987) Exp Cell Res 168: 325–337

    Google Scholar 

  • Pekkala D, Silver JC (1990) Exp Cell Res 187: 16–24

    Google Scholar 

  • Pekkala D, Heath IB, Silver JC (1984) Mol Cell Biol 4: 1198–1205

    Google Scholar 

  • Rebbe NF, Hickman WS, Ley TJ, Strafford DW, Hickman S (1989) J Biol Chem 264: 15006–15011

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Silver JC, Horgen PA (1974) Nature 249: 252–254

    Google Scholar 

  • Silver JC, Andrews DR, Pekkala D (1983) Can J Biochem Cell Biol 61: 447–455

    Google Scholar 

  • Timberlake WE, Orr WC (1984) In: Goldberger RF, Yamamoto KR (eds) Biological regulation of development, vol 3 B. Plenum Publishing Corp, New York, pp 255–283

    Google Scholar 

  • Ullrich SJ, Robinson EA, Law LW, Willinghan M, Appela E (1986) Proc Natl Acad Sci USA 83: 3121–3125

    Google Scholar 

  • Ullrich SJ, Moore SK, Appella E (1989) J Biol Chem 264: 6810–6816

    Google Scholar 

  • Walter T, Drabent B, Krebs H, Tomalak M, Heiss S, Benecke B-J (1989) Gene 83: 105–115

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology and Division of Life Sciences, University of Toronto, Scarborough Campus, M1C 1A4, Ontario, Canada

    Shelley A. Brunt & Julie C. Silver

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  1. Shelley A. Brunt
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  2. Julie C. Silver
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Communicated by H. Bertrand

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Brunt, S.A., Silver, J.C. Molecular cloning and characterization of two distinct hsp 85 sequences from the steroid responsive fungus Achlya ambisexualis . Curr Genet 19, 383–388 (1991). https://doi.org/10.1007/BF00309599

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  • DOI: https://doi.org/10.1007/BF00309599

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