Summary
Chromosomes have been studied in protoplast-derived potato plants of the tetraploid cultivars Maris Bard and Fortyfold. A high degree of aneuploidy was found amongst the regenerants of both cultivars but the nature of the chromosome variation differed. The Maris Bard regenerants were characterised by high chromosome numbers, a wide range of aneuploidy (46–92) and a low percentage of plants with the normal chromosome number (2n = 48), whereas a much higher proportion of the Fortyfold regenerants had 48 chromosomes and the variants were within a more limited aneuploid range. In both cultivars chromosome variation was found between calluses, within calluses and even within shoot cultures. The origin of the chromosome variation and the differences found between the two cultivars are discussed.
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Literature
Bayliss, M.W. (1980): Chromosomal variation in plant tissues in culture. Intern. Rev. Cytol. Suppl. 11A, 113–144
Constantin, M.J. (1981): Chromosome instability in cell and tissue cultures and regenerated plants. Environ Exp. Bot. 21, 359–368
D'Amato, F. (1977): Cytogenetics of differentiation in tissue and cell cultures. In: Applied and fundamental aspects of plant cell, tissue, and organ culture (eds. Reinert, J.; Bajaj, Y.P.S.), pp. 343–357. Berlin, Heidelberg, New York: Springer
D'Amato, F. (1978): Chromosome number variation in cultured cells and regenerated plants. In: Frontiers of plant tissue culture (ed. Thorpe, J.A.), pp. 287–295. Intern. Ass. Plant Tissue Culture, University of Calgary Offset Printing Services, Calgary, Canada
Larkin, P.J.; Scowcroft, W.R. (1981): Somaclonal variation — a novel source of variability from cell cultures. Theor. Appl. Genet. 60, 197–214
Matern, U.; Strobel, G.; Shepard, J.F. (1978): Reaction to phytotoxins in a potato population derived from mesophyll protoplasts. Proc. Natl. Acad. Sci. USA 75, 4935–4939
Orton, T.J. (1980): Chromosomal variability in tissue cultures and regenerated plants of Hordeum. Theor. Appl. Genet. 56, 101–112
Sacristan, M.D. (1971): Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L.) Wallr. Chromosoma 33, 273–283
Secor, G.A.; Shepard, J.F. (1981): Variability of protoplastderived potato clones. Crop Sci. 21, 102–105
Shepard, J.F.; Bidney, D.; Shahin, E. (1980): Potato protoplasts in crop improvement. Science 208, 17–24
Shepard, J.F. (1980): Mutant selection and plant regeneration from potato mesophyll protoplasts. In: Genetic improvement of crops/emergent techniques (eds. Rubenstein, I.; Gengenbach, R.L.; Philips, R.L.; Green, C.E.), pp. 185–219. Univ. of Minnesota Press, Minneapolis, MN
Thomas, E. (1981): Plant regeneration from shoot-culture derived protoplasts of tetraploid potato (Solanum tuberosum cv. Maris Bard). Plant Sci. Lett. 23, 84–88
Thomas, E.; Bright, S.W.J.; Franklin, J.; Lancaster, V.; Miflin, B.J.; Gibson, R. (1982): Variation amongst protoplast-derived potato plants (Solanum tuberosum cv. Maris Bard). Theor. Appl. Genet. 62, 65–68
Wenzel, G.; Schieder, O.; Przewozny, T.; Sopory, S.K.; Melchers, G. (1979): Comparison of single cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor. Appl. Genet. 55, 49–55
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Communicated by R. Riley
We regret to report the death of Emrys Thomas since the initiation of this work
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Karp, A., Nelson, R.S., Thomas, E. et al. Chromosome variation in protoplast-derived potato plants. Theoret. Appl. Genetics 63, 265–272 (1982). https://doi.org/10.1007/BF00304006
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DOI: https://doi.org/10.1007/BF00304006