Summary
A Neurospora crassa mutation, mei-2, affecting meiosis and mutagen sensitivity, was characterized for its effect on meiotic recombination and chromosome pairing. Results from homozygous mei-2 crosses involving distant markers on the same chromosome demonstrated a drastic reduction in meiotic recombination. However, mitotic recombination continued to occur. Cytological observations indicated that pairing of homologous chromosomes in zygotene was greatly reduced or absent, resulting in aberrant segregation at anaphase I and often at subsequent divisions as well. The few mature ascospores produced were frequently disomic for one or more chromosomes.
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References
Alani E, Padmore R, Kleckner N (1990) Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination. Cell 61:419–436
Baker BS, Carpenter ATC, Esposito MS, Esposito RE, Sandler L (1976) The genetic control of meiosis. Annu Rev Genet 10:53–134
Carpenter ATC (1984) Genetic control of meiosis. In: Bennett MD, Gropp A, Wolf U (eds) Chromosomes Today 8, George Allen and Unwin, London, pp 70–79
Coyle MB, Pittenger TH (1965) Mitotic recombination in pseudowild types of Neurospora. Genetics 52:609–625
Davis RH, de Serres FJ (1970) Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol 17A:79–143
Foss HM, Selker EU (1991) Efficient DNA pairing in a Neurospora mutant defective in chromosome pairing. Mol Gen Genet 231:49–52
Golubovskaya IN (1979) Genetic control of meiosis. Int Rev Cytol 58:247–290
Haynes RH, Kunz BA (1981) DNA repair and mutagenesis in yeast. In: Strathern JN, Jones EM, Brach JR (eds) The molecular biology of the yeast Saccharomyces: life cycle and inheritance. Cold Spring Harbor Laboratory Press, New York, pp 371–414
Hollingsworth NM, Byers B (1989) HOP1: a yeast meiotic pairing gene. Genetics 121:445–462
Klapholz S, Waddell CS, Esposito RE (1985) The role of the SPO11 gene in meiotic recombination in yeast. Genetics 110:187–216
LeClerc G (1946) Occurrence of mitotic crossing over without meiotic crossing over. Science 103:553–554
Lu BC, Galeazzi DR (1978) Light and electron microscope observations of a meiotic mutant of Neurospora crassa. Can J Bot 56:2694–2706
Malone RE, Montelone BA, Edwards C, Carney K, Hoekstra MF (1988) A reexamination of the role of the RAD52 gene in spontaneous mitotic recombination. Curr Genet 14:211–223
Menees TM, Roeder GS (1989) MEI4, a yeast gene required for meiotic recombination. Genetics 123:675–682
Moreau PJF, Zickler D, Leblon G (1985) One class of mutants with disturbed centromere cleavage and chromosome pairing in Sordaria macrospora. Mol Gen Genet 198:189–197
Newmeyer D, Galeazzi DR (1977) The instability of neurospora duplication Dp(IL→IR) H4250 and its genetic control. Genetics 85:461–487
Newmeyer D, Taylor CW (1967) A pericentric inversion in Neurospora, with unstable duplication progeny. Genetics 56:771–791
Perkins DD, Radford A, Newmeyer D, Björkman M (1982) Chromosomal loci of Neurospora crassa. Microbiol Rev 46:426–570
Raju NB (1978) Meiotic nuclear behavior and ascospore formation in five homothallic species of Neurospora. Can J Bot 56:754–763
Raju NB (1980) Meiosis and ascospore genesis in Neurospora. Eur J Cell Biol 23:208–223
Raju NB (1986) Postmeiotic mitoses without chromosome replication in a mutagen-sensitive Neurospora mutant. Exp Mycol 10:243–251
Raju NB (1992) Genetic control of the sexual cycle in Neurospora. Mycological research (in press)
Raju NB, Newmeyer D (1977) Giant ascospores and abnormal croziers in a mutant of Neurospora crassa. Exp Mycol 1:152–165
Raju NB, Perkins DD (1978) Barren perithecia in Neurospora crassa. Can J Genet Cytol 20:41–59
Resnick MA (1987) Investigating the genetic control of biochemical events in meiotic recombination. In: Moens P (ed) Meiosis. Academic Press, NY, pp 157–210
Resnick MA, Nitiss J, Edwards C, Malone RE (1986) Meiosis can induce recombination in rad52 mutants of Saccharomyces cerevisiae. Genetics 113:531–550
Schroeder AL (1970) Ultraviolet-sensitive mutants of Neurospora. I. Genetic basis and effect on recombination. Mol Gen Genet 107:291–304
Schroeder AL (1986) Chromosome instability in mutagen sensitive mutants of Neurospora. Curr Genet 10:381–387
Schroeder AL (1988) Use of Neurospora to study DNA repair. In: Friedberg EC, Hanawalt PC (eds) DNA repair, a laboratory manual of research procedures, vol 3. Marcel Dekker, New York and Basel, pp 77–98
Schroeder AL, Olson LD (1983) Mutagen sensitivity of Neurospora meiotic mutants. Can J Genet Cytol 25:16–25
Selker EU (1990) Premeiotic instability of repeated sequences in Neurospora crassa. Annu Rev Genet 24:579–613
Smith DA (1974) Unstable diploids of Neurospora and a model for their somatic behavior. Genetics 76:1–17
Smith DA (1975) A mutant affecting meiosis in Neurospora. Genetics 80:125–133
Westergaard M, Mitchell HK (1947) Neurospora. V. A synthetic medium favoring sexual reproduction. Am J Bot 34:573–577
Zickler D, deLares L, Moreau PJF, Leblon G (1985) Defective pairing and synaptonemal complex formation in a Sordaria mutant (spo-44) with a translocated segment of the nucleolus organizer. Chromosoma 92:37–47
Zolan ME, Tremel CJ, Pukkila PJ (1988) Production and characterization of radiation-sensitive meiotic mutants of Coprinus cinereus. Genetics 120:379–387
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Communicated by B. Kilbey
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Schroeder, A.L., Raju, N.B. mei-2, a mutagen-sensitive mutant of Neurospora defective in chromosome pairing and meiotic recombination. Molec. Gen. Genet. 231, 41–48 (1991). https://doi.org/10.1007/BF00293819
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DOI: https://doi.org/10.1007/BF00293819