Summary
During the transition of Escherichia coli cultures from exponential growth to stationary phase, the pre-existing RNA polymerase was found to be converted into at least three different holoenzyme forms, which could be separated by phosphocellulose column chromatography. The relative levels of these three holoenzyme forms changed depending on the phase of cell growth. The altered stationary phase forms of RNA polymerase showed promoter recognition properties that were different from those of the holoenzyme from exponentially growing cells. Enzyme reconstitution experiments showed that the altered promoter selectivity was due to modification of the core enzyme. We propose that modulation of RNA polymerase plays a role in the global switch of gene expression during the transition from exponential growth to stationary phase.
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References
Aldea M, Garrido T, Hernandez-Chico C, Vicente M, Kushner SR (1989) Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene. EMBO J 8:3923–3931
Aldea M, Garrido T, Pla J, Vicente M (1990) Division genes in Escherichia coli are expressed coordinately to cell septum requirements by gearbox promoters. EMBO J 9:3787–3894
Atlung T, Nielsen A, Hansen FG (1989) Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growthphase dependent gene expression in Escherichia coli. J Bacteriol 171:1683–1691
Buchanan CE, Sowell MO (1982) Synthesis of penicillin-binding protein 6 by stationary-phase Escherichia coli. J Bacteriol 151:491–494
De la Rosa MA, de Pedro MA, Vazquez D (1982) Modification of penicillin-binding proteins of Escherichia coli associated with changes in the state of growth of the cells. FEMS Lett 14:91–94
Diaz-Guerra L, Moreno F, Millan JS (1989) Cloning and mapping of the genetic determinants for microcin C7 production and immunity. J Bacteriol 171:2960–2968
Enami M, Ishihama A (1982) Biosynthesis of RNA polymerase in Escherichia coli, XII. Noncoordinate synthesis of core enzyme subunits after suppression of cell growth. Mol Gen Genet 185:373–387
Fujita N, Nomura T, Ishihama A (1987) Promoter selectivity of Escherichia coli RNA polymerase. Purification and properties of holoenzyme containing the heat-shock σ subunit. J Biol Chem 262:1855–1859
Fukuda R, Iwakura Y, Ishihama A (1974) Heterogeneity of RNA polymerase in Escherichia coli. J Mol Biol 83:353–367
Gonzalez N, Wiggs J, Chamberlin M (1977) A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase. Arch Biochem Biophys 182:404–408
Helmann JD, Chamberlin M (1988) Structure and function of bacterial sigma factors. Annu Rev Biochem 57:839–872
Hernandes-Chico C, Millan JS, Kolter R, Moreno F (1986) Growth phase and OmpR regulation of transcription of microcin B17 genes. J Bacteriol 167:1058–1065
Ishihama A (1981) Subunit assembly of Escherichia coli RNA polymerase. Adv Biophys 21:163–173
Ishihama A (1988) Promoter selectivity of prokaryotic RNA polymerases. Trends Genet 4:282–286
Ishihama A, Ito K (1972) Subunits of RNA polymerase in function and structure, II. Reconstitution of Escherichia coli RNA polymerase from isolated subunits. J Mol Biol 72:111–123
Kajitani M, Ishihama A (1983a) Determination of the promoter strength in the mixed transcription system: Promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli. Nucl Acids Res 11:671–686
Kajitani M, Ishihama A (1983b) Determination of the promoter strength in the mixed transcription system, II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli. Nucleic Acids Res 11:3837–3887
Kawakami K, Saitoh T, Ishihama A (1979) Biosynthesis of RNA polymerase in Escherichia coli. IX. Growth-dependent variations in the synthesis rate, content and distribution of RNA polymerase. Mol Gen Genet 174:107–116
Kobayashi M, Nagata K, Ishihama A (1990) Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter −35 region on promoter strength. Nucleic Acids Res 18:7367–7372
Wada A, Yamazaki Y, Fujita N, Ishihama A (1990) Structure and probable genetic location of a “ribosome modulation factor” associated with 1005 ribosomes in stationary-phase. Proc Natl Acad Sci 87:2657–2661
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This is paper no. 9 in the series “Promoter Selectivity of Escherichia coli RNA polymerase”. Paper 8 is Kobayashi et al. (1990)
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Ozakil, M., Wada, A., Fujita, N. et al. Growth phase-dependent modification of RNA polymerase in Escherichia coli . Molec. Gen. Genet. 230, 17–23 (1991). https://doi.org/10.1007/BF00290644
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DOI: https://doi.org/10.1007/BF00290644