Summary
The nucleotide sequence of the melB gene coding for the Na+(Li+)/melibiose symporter of Salmonella typhimurium LT2 was determined, and its amino acid sequence was deduced. It consists of 1428 bp, corresponding to a protein of 476 amino acid residues (calculated molecular weight 52800). The amino acid sequence is homologous to that of the melibiose permease of Escherichia coli K12, with 85% identical residues. All, except one, of the amino acid residues that have been reported to be important for cation or substrate recognition in the melibiose permease of E. coli are conserved in the melibiose permease of S. typhimurium. In addition, part of the sequence resembles the lactose permease of Streptococcus thermophilus, the animal glucose transporter (GLUT1), the plasmid-coded raffinose permease (RafB), and the NADH-ubiquinone oxidoreductase chain 4 (Nuo4) of Aspergillus amstelodami.
Similar content being viewed by others
References
Alting-Mess MA, Short JM (1989) pBluescriptll: gene mapping vectors. Nucleic Acids Res 17:9494
Andersson SGE, Kurland CG (1990) Codon preferences in freeliving microorganisms. Microbiol Rev 54:198–210
Aslanidis C, Schmid K, Schmitt R (1989) Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli. J Bacteriol 171:6753–6763
Berns KI, Thomas Jr Ca (1965) Isolation of high molecular weight DNA from Haemophilus influenzae. J Mol Biol 11:476–490
Botfield MC, Wilson TH (1988) Mutations that simultaneously alter both sugar and cation specificity in the melibiose carrier of Escherichia coli. J Biol Chem 263:12909–129015
Botfield MC, Noguchi K, Tsuchiya T, Wilson TH (1992) Membrane topology of the melibiose carrier of Escherichia coli. J Biol Chem 267:1818–1822
Bullock WO, Fernandez JM, Short JM (1987) X11-blue: A high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5:376–379
Eisenberg D, Schwarz E, Komaromy M, Wall R (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J Mol Biol 179:125–142
Ikemura T (1982) Correlation between the abundance of yeast transfer RNAs and the occurrence of the respective codons on protein genes. J Mol Biol 158:573–597
Ishikawa T, Hama H, Tsuda M, Tsuchiya T (1987) Isolation and properties of a mutant of Escherichia coli possessing defective Na+/H+ antiporter. J Biol Chem 262:7443–7446
Kaback HR (1987) Use of site-directed mutagenesis to study the mechanism of a membrane transport protein. Biochemistry 26:2071–2076
Kawakami T, Akizawa Y, Ishikawa T, Shimamoto T, Tsuda M, Tsuchiya T (1988) Amino acid substitutions and alteration in cation specificity in the melibiose carrier of Escherichia coli. J Biol Chem 263:14276–14280
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
Lennox ES (1955) Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190–206
Levinthal M (1971) Biochemical studies of melibiose metabolism in wild type and mel mutant strains of Salmonella typhimurium. J Bacteriol 105:1047–1052
Liljestrom P, Liljestrom P (1987) Nucleotide sequence of the me/A gene, coding for α-galactosidase in Escherichia coli K-12. Nucleic Acids Res 15:2213–2220
Lopilato J, Tsuchiya T, Wilson TH (1978) Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli. J Bacteriol 134:147–156
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Maloney PC, Ambudkar SV, Anantharam V, Sonna LA, Varadhachary AV (1990) Anion-exchange mechanism in bacteria. Microbiol Rev 54:1–17
Mueckler M, Caruso C, Baldwin SA, Panico M, Blench I, Morris HR, Allard WJ, Lienhard GE, Lodish HF (1985) Sequence and structure of a human glucose transporter. Science 229:941–945
Niiya S, Moriyama Y, Futai M, Tsuchiya T (1980) Cation coupling to melibiose transport in Salmonella typhimurium. J Bacteriol 144:192–199
Niiya S, Yamasaki K, Wilson TH, Tsuchiya T (1982) Altered cation coupling to melibiose transport in mutants of Escherichia coli. J Biol Chem 257:8902–8906
Poolman B, Royer TJ, Mainzer ST, Schmidt BF (1989) Lactose transport system of Streptococcus thermophilus: a hybrid protein with homology to the melibiose carrier and enzyme III of phosphoenolpyruvate-dependent phosphotransferase system. J Bacteriol 171:244–253
Poucher T, Sarker HK, Bassilana M, Kaback HR, Leblanc G (1990) Histidine-94 is the only important histidine residue in the melibiose permease of Eseherichia coli. Proc Natl Acad Sci USA 87:468–472
Prestidge S, Pardee A (1965) A second permease for methyl-thio-β-d-galactoside in Escherichia coli. Biochim Biophys Acta 100:591–593
Sal GD, Mantioletti G, Schneider C (1988) A one-tube plasmid mini-preparation suitable for sequencing. Nucleic Acids Res 16:9878
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shine J, Dalgarno L (1974) The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346
Shiota S, Yamane Y, Futai M, Tsuchiya T (1985) Escherichia coli mutants possessing an Li+-resistant melibiose carrier. J Bacteriol 162:106–109
Stern MJ, Ames GF-L, Smith NH, Robinson EC, Higgins CF (1984) Repetitive extragenic palindromic sequences: A major component of the bacterial genomes. Cell 37:1015–1026
Stock J, Roseman S (1971) A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun 44:132–138
Tanaka S, Lerner SA, Lin ECC (1967) Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol 93:642–648
Thelen P, Tsuchiya T, Goldberg EB (1991) Characterization and mapping of a major Na+/H+ antiporter gene of Escherichia coli. J Bacteriol 173:6553–6557
Tsuchiya T, Wilson TH (1978) Cation-sugar cotransport in the melibiose transport system of Escherichia coli. Membr Biochem 2:63–79
Tsuchiya T, Oho M, Shiota-Niiya S (1983) Lithium ion-sugar cotransport via the melibiose transport system in Escherichia coli. J Biol Chem 258:12765–12767
von Heijne G (1986) The distribution of positively charged residues in bacterial inner membrane proteins correlates with the transmembrane topology. EMBO J 5:3021–3027
Yazyu H, Shiota-Niiya S, Shimamoto T, Kanazawa H, Futai M, Tsuchiya T (1984) Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem 259:4320–4326
Yazyu H, Shiota S, Futai M, Tsuchiya T (1985) Alteration in cation specifity of the melibiose transport carrier of Escherichia coli due to replacement of proline 122 with serine. J Bacteriol 162:933–937
Author information
Authors and Affiliations
Additional information
Communicated by J. Lengeler
The nucleotide sequence reported in this paper has been submitted to the DDBJ/GenBank/EMBL Data Bank with accession number X62101
Rights and permissions
About this article
Cite this article
Mizushima, K., Awakihara, S., Kuroda, M. et al. Cloning and sequencing of the meIB gene encoding the melibiose permease of Salmonella typhimurium LT2. Molec. Gen. Genet. 234, 74–80 (1992). https://doi.org/10.1007/BF00272347
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00272347