Summary
A whey fermentation by Kluyveromyces fragilis was scaled-up to a 1000-dm3 stirred fermentor, by varying the stirrer speed, the air-flow rate and the initial concentration of lactose. Its evolution was simulated by applying the same unstructured model (consisting of a microbial specific growth rate of pseudo-first order with respect to the COD concentration and constant biomass yield per unit COD removed) set up in previous experiments using 8- to 80-dm3 fermentors. Despite the great scale-up ratios, very different operating conditions, and geometric dissimilarity, a series of empirical regressions previously developed allowed approximate, but acceptable prediction of the stoichiometric and kinetic coefficients of the above mathematical model, thus confirming the capability of this model to provide a reliable basis for further scale-up of this fermentation process to a production scale.
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References
Aiba S, Humphrey AE, Millis NF (1973) Biochemical engineering. 2nd edn. Academic Press, New York, pp 110–114
ASTM Standards (1964) Industrial water; atmospheric analysis. Part 23-D 1252:233–264
Beausejour D, Leduy A, Ramalho RS (1981) Batch cultivation of Kluyveromyces fragilis in cheese whey. Can J Chem Eng 59:522–526
Blakebrough N, Moresi M (1981a) Scale-up of whey fermentation in a pilot-scale fermenter. Eur J Appl Microbiol Biotechnol 12:173–178
Blakebrough N, Moresi M (1981b) Modelling of the process yields of a whey fermentation. Eur J Appl Microbiol Biotechnol 13:1–9
Cooper CM, Fernstrom GA, Miller SA (1944) Performance of agitated gas-liquid contactors. Ind Eng Chem 36:504–509
Holmberg A, Ranta J (1982) Procedure for parameter and state estimation of microbial growth process models. Automatica 18:181–193
Holmberg A, Sievanen R, Calberg G (1980) Fermentation of Bacillus thuringiensis for exotoxin production — process analysis study. Biotechnol Bioeng 22:1707–1724
Moresi M, Patete M (1988a) Cinetica del processo di crescita di K. fragilis su siero di latte. Ann Microbiol Enzimol 38:1–13
Moresi M, Patete M (1988b) K L a prediction in conventional stirred fermenters. J Chem Technol Biotechnol 42:197–210
Moresi M, Sebastiani E (1979) Optimization of whey fermentation in a shaken-flask fermenter. Eur J App Microbiol Biotechnol 8:63–71
Moresi M, Nacca C, Palleschi C (1979) Factor analysis in a whey fermentation in a shaken-flask fermenter. Eur J Appl Microbiol Biotechnol 8:49–61
Moresi M, Colicchio A, Sansovini F (1980a) Optimization of whey fermentation in jar fermenter. Eur J Appl Microbiol Biotechnol 9:173–183
Moresi M, Colicchio A, Sansovini F, Sebastiani E (1980b) Chemical oxygen demand reduction in a whey fermentation. Eur J Appl Microbiol Biotechnol 9:261–274
Moresi M, Clementi F, Rossi J (1987) Kinetics of untreated orange peel utilisation by F. avenaceum. J Chem Technol Biotechnol 40:233–249
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Moresi, M., Patete, M. & Trunfio, A. Scaling-up of a batch whey fermentation by Kluyveromyces fragilis . Appl Microbiol Biotechnol 31, 495–501 (1989). https://doi.org/10.1007/BF00270783
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DOI: https://doi.org/10.1007/BF00270783