Skip to main content
SpringerLink
Log in

Preferential delection of a specific region of mitochondrial DNA in Saccharomyces cerevisiae by ethidium bromide and 3-carbethoxy-psoralen

Directional retention of DNA sequence

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Grande strains of Saccharomyces cerevisiae were mutagenized either by ethidium bromide or by 3-carbethoxy-psoralen (a monofunctional furocoumarin derivative) activated by 365 nm light. 973 primary rho− clones induced were randomly collected and analyzed individually for the presence or absence of fifteen mitochondrial genetic markers.

  1. 1.

    Under mild conditions of mutagenesis, 83% of the primary clones showed single-deletion genotypes; a unique order of 14 markers could be deduced from the patterns of the deletion. The gene order confirmed our previous map constructed from the analysis of established non-random petite clones. From the frequencies of disjunction between markers, the distances separating 14 mitochondrial markers were estimated.

  2. 2.

    One region, carrying oxi-3, pho-1 and mit 175 loci, was preferentially lost in rho− mutants: there is a strong constraint in the frequencies of various genotypes found in rho− clones. On each side of this particular region, a bidirectionally oriented pattern of retention of markers is observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

mtDNA:

mitochondrial DNA

EB:

ethidium bromide

3 CPs:

3-carbethoxy-psoralen

rho:

cytoplasmic respiratory deficient mutants, or “petite”

References

  • Averbeck, D., Moustacchi, E.: Biological effects and repair of damage photoinduced by a derivative of psoralen substituted in the 3,4 reaction. II. Induction of reversion, mitotic recombination and cytoplasmic (ϱ −) mutation in yeast. Biochim. biophys. Acta (Amst.) (in press) (1978)

  • Averbeck, D., Moustacchi, E., Bisagni, E.: Biological effects and repair of damage photoinduced by a derivative of psoralen substituted at the 3,4 reaction site. I. Photoreactivity of this compound and lethal effect in yeast. Biochim. biophys. Acta (Amst.) (in press) (1978)

  • Bolotin-Fukuhara, M. Faye, G., Fukuhara, H.: Localization of some mitochondrial mutations in relation to transfer and ribosomal RNA genes in Saccharomyces cerevisiae. In: The genetic function of mitochondrial DNA (C. Saconne and A.M. Kroon, eds.), pp. 243–250. Amsterdam: Elsevier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Bolotin-Fukuhara, M., Faye, G., Fukuhara, H.: Temperature-sensitive respiratory-deficient mitochondrial mutations: Isolation and genetic mapping. Molec. gen. Genet. 152, 295–305 (1977)

    Google Scholar 

  • Bolotin-Fukuhara, M., Fukuhara H.: Modified recombination and transmission of mitochondrial genetic markers in rho minus mutants of Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 73, 4608–4612 (1976)

    Google Scholar 

  • Borst, P.: Structure of petite mtDNA; Prospects for mitochondrial gene isolation, mapping and sequencing. In: The biogenesis of mitochondria (A.M. Kroon and C. Saccone, eds.) pp. 147–156 New York: Academic Press 1974

    Google Scholar 

  • Casey, J., Cohen, M., Rabinowitz, M., Fukuhara, H., Getz, G.S.: Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast. J. molec. Biol. 63, 431–440 (1972)

    Google Scholar 

  • Casey, J., Hsu, U., Rabinowitz, M., Getz, G.S., Fukuhara, H.: Transfer RNA genes in the mitochondrial DNA of cytoplasmic petite mutants of Saccharomyces cerevisiae. J. molec. Biol. 88, 717–733 (1974)

    Google Scholar 

  • Deutsch, J., Dujon, B., Netter, P., Petrochilo, E., Slonimski, P.P., Bolotin-Fukuhara, M., Coen, D.: Mitochondrial genetics. VI. The petite mutation in Saccharomyces cerevisiae: interrelations between the loss of the rho+ factor and the loss of the drug resistance mitochondrial genetic markers. Genetics 76, 195–219 (1974)

    Google Scholar 

  • Faye, G., Fukuhara, H., Grandchamp, C., Lazowska, J., Michel, F., Casey, J., Getz, G.S., Locker, J., Rabinowitz, M., Bolotin-Fukuhara, M., Coen, D., Deutsch, J., Dujon, B., Netter, P., Slonimski, P.P., Mitochondrial nucleic acids in the petite colonie mutants: deletions and repetitions of genes. Biochimie 55, 779–792 (1973)

    Google Scholar 

  • Faye, G., Kujawa, C., Fukuhara, H.: Physical and genetic organization of petite and grande yeast mitochondrial DNA. IV. In vivo transcription products of mitochondrial DNA and localization of 23S ribosomal RNA in petite mutants of Saccharomyces cerevisiae. J. molec. Biol. 88, 185–203 (1974)

    Google Scholar 

  • Faye, G., Bolotin-Fukuhara, M., Fukuhara, H.: Mitochondrial mutations that affect mitochondrial transfer ribonucleic acids in Saccharomyces cerevisiae. In: The genetics and the biogenesis of chloroplasts and mitochondria (T. Bücher, W. Neupert, W. Sebald and S. Werner, eds.), pp. 547–555. Amsterdam: North-Holland 1976

    Google Scholar 

  • Fukuhara, H., Bolotin-Fukuhara, M., Hsu, H.U., Rabinowitz, M.: Deletion mapping of mitochondrial transfer RNA genes in Saccharomyces cerevisiae by means of cytoplasmic petite mutants. Molec gen. Genet. 145, 7–17 (1976)

    Google Scholar 

  • Fukuhara, H., Wesolowski, M.: Preferential loss of a specific region of mitochondria DNA by rho− mutation. In: Genetics and biogenesis of mitochondria (W. Bandlow et al., eds.), pp. 123–131 Berlin: De Gruyter 1977

    Google Scholar 

  • Juliani, M.H., Hixon, S., Moustacchi, E.: Mitochondrial genetic damage induced in yeast by a photoactivated furocoumarin in combination with ethidium bromide and ultraviolet light. Molec. gen. Genet. 145, 249–254 (1976)

    Google Scholar 

  • Lancashire, W.E.: Mitochondrial mutations conferring heat or cold sensitivity in Saccharomyces cerevisiae. In: Genetics and biogenesis of chloroplasts and mitochondria (T. Bücher et al., eds.), pp. 481–490. Biomedical Press. Amsterdam: Elsevier-Northholland 1976

    Google Scholar 

  • Lang, B., Burger, G., Bandlow, W., Kaudewitz, F., Schweyen, R.J.: Antimycin- and funiculosin-resistant mutants in Saccharomyces cerevisiae: New markers on mitochondrial DNA. In: Genetics and biogenesis of chloroplasts and mitochondria (Th. Bücher et al., eds), pp. 461–465. Amsterdam: Elsevier-North-Holland Biomedical Press 1976

    Google Scholar 

  • Mathews, S., Schweyen, R.J., Kaudewitz, F.: Preferential loss or retention of mitochondrial genes in rho− clones. In: Genetics and biogenesis of mitochondria (W. Bandlow et al., ed.), pp. 123–131. Berlin: De Gruyter 1977

    Google Scholar 

  • Molloy, P.L., Linnane, A.W., Lukins, H.B.: Biogenesis of mitochondria: Analysis of deletion of mitochondrial antibiotic resistance markers in petite mutants of Saccharomyces cerevisiae. J. Bact. 122, 7–18 (1975)

    Google Scholar 

  • Morimoto, R., Lewin, A., Hsu, H.J., Rabinowitz, M., Fukuhara, H.: Restriction endonuclease analysis of mitochondrial DNA from grande and genetically characterized cytoplasmic petite clones of Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 72, 3868–3872 (1975)

    Google Scholar 

  • Morimoto, R., Lewin, A., Merten, S., Rabinowitz, M.: Restriction endonuclease mapping and analysis of grande and mutant yeast mitochondrial DNA. In: Genetics and biogenesis of chloroplasts and mitochondira (T. Bücher et al., eds), pp. 519–524. Amsterdam, Elsevier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Morimoto, R., Lewin, A., Rabinowitz, M.: Restriction cleavage map of mitochondrial DNA from the yeast Saccharomyces cerevisiae. Nucl. Acids Res. 4, 2331–2351 (1977)

    Google Scholar 

  • Musajo, L., Rodighiero, G., Caporale, G., Dall'Acqua, F., Marciani, S., Bordin, F., Bahichetti, F., Bevilacqua, R.: In: Sunlight and mannormal and abnormal photobiologic response (M.A. Pathak et al., eds), pp. 369–387, Tokyo: University of Tokyo Press 1974

    Google Scholar 

  • Nagley, P., Molloy, P.L., Lukins, H.B., Linnane, A.W.: Studies on mitochondrial gene purification using petite mutants of yeast: characterization of mutants enriched in ribosomal RNA cistrons. Biochem. biophys. Res. Commun. 57, 232–238 (1974)

    Google Scholar 

  • Nagley, P., Sriprakash, K.S., Rytka, J., Choo, B., Trembach, M.K., Lukins, H.B., Linnane, A.W.: Physical mapping of genetic markers in yeast mitochondrial genome. In: The genetic function of mitochondrial DNA. (C. Saccone and A.M. Kroon, eds.), pp. 231–242, Amsterdam: Elsevier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Piperno, G., Fonty, G., Bernardi, G.: The mitochondrial genome of “grande” yeast cells. II. Investigations on the compositional heterogeneity of mitochondrial DNA. J. molec. Biol. 65, 191–205 (1972)

    Google Scholar 

  • Prunell, A., Bernardi, G.: The mitochondrial genome of wild type yeast cells. IV. Genes and spacers. J. molec. Biol. 86, 825–841 (1974)

    Google Scholar 

  • Rytka, J., English, K.J., Hall, R.M., Linnane, A.W., Lukins, H.B.: The isolation and simultaneous physical mapping of mitochondrial mutations affecting respiratory complexes. In: Genetics and biogenesis of chloroplasts and mitochondria (T. Bücher et al., eds), pp. 427–434 Amsterdam: Elsvier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Sanders, J.P.M., Borst, P.: The organization of genes in yeast mitichondrial DNA. IV. Analysis of (dA-dT) clusters in yeast mitochondrial DNA by poly (U)-sephadex chromatography. Molec. gen. Genet. 157, 263–269 (1977)

    Google Scholar 

  • Sanders, J.P.M., Heyting, C., Difranco, A., Borst, P., Slonimski, P.: The organization of genes in yeast mitochondrial DNA. In: The genetic function of mitochondrial DNA (C. Saccone and A.M. Kroon, eds.), pp. 259–272. Amsterdam: Elsevier/North-Holland Biomedical Press 1976

    Google Scholar 

  • Schweyen, R.J., Steyer, U., Kaudewitz, F., Dujon, B., Slonimski, P.: Mapping of mitochondrial genes in Saccharomyces cerevisiae: Population and pedigree analysis of retention or loss of four genetic markers in rho− cells. Molec. gen. Genet. 146, 117–132 (1976b)

    Google Scholar 

  • Schweyen, R.J., Weiss-Brummer, B., Backhaus, B., Kaudewitz, F.: Lacolization of seven gene loci on a circular map of the mitochondrial genome of Saccharomyces cerevisiae. In: The genetic function of mitochondrial DNA (C. Saccone and A.M. Kroon, eds.), pp. 251–258, Amsterdam: Elsevier/North-Holland Biomedical Press 1976a

    Google Scholar 

  • Slonimski, P., Tzagoloff, A.: Localization in yeast mitochondrial DNA of mutations expressed in a deficiency of cytochrome oxidase and for coencyme QH2-cytochrome C reductase. Europ. J. Biochem. 61, 27–41 (1976)

    Google Scholar 

  • Strauss, B., Coyle, M., McMahon, M., Kato, K., Dolynink, M.: DNA synthesis, repair, and chromosome breaks in eucaryotic cells. In: Molecular and cellular repair processes (R.F. Beers, R.M. Herriott and R.C. Tilghman, eds.), pp. 1–124 Baltimore: Johns Hopkins University Press 1972

    Google Scholar 

  • Waters, R., Moustacchi, E.: The fate of ultraviolet induced pyrimidine dimers in the mitochondrial DNA of Saccharomyces cerevisiae following various post-irradiation cell treatments. Biochim. biophys. Acta (Amst.) 366, 241–250 (1974)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Section de Biologie, Fondation Curie-Institut du Radium, Bâtiment 110, F-91405, Orsay, France

    H. Fukuhara, E. Moustacchi & M. Wesolowski

Authors
  1. H. Fukuhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Moustacchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Wesolowski
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukuhara, H., Moustacchi, E. & Wesolowski, M. Preferential delection of a specific region of mitochondrial DNA in Saccharomyces cerevisiae by ethidium bromide and 3-carbethoxy-psoralen. Molec. Gen. Genet. 162, 191–201 (1978). https://doi.org/10.1007/BF00267876

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267876

Keywords

Search

Navigation