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Two isoaccepting seryl tRNAs coded by separate mitochondrial genes in yeast

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Summary

In S. cerevisiae four isoacceptor mitochondrial tRNAs for serine have been separated by reversed phase chromatography. At least two of these species are products of different genes. In this work the deletion mapping technique has been used to locate two genes for tRNAser. The gene for tRNAser previously localized in the oli I region of the mitochondrial genome has been found to code for tRNA 2ser , and another gene coding for tRNA 1ser has been detected in the region where most of other tRNA genes are found. Results of fine mapping experiments allowed to localize this gene in the proximity of the gene for tRNAarg.

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Authors and Affiliations

  1. Centro Acidi Nucleici, Università di Roma, Rome, (Italy)

    Elisabetta Colletti, Laura Frontali & Claudio Palleschi

  2. Section de Biologie, Institut Curie, Orsay, France

    Micheline Wesolowski & Hiroshi Fukuhara

  3. Istituto di Fisiologia Generale, Facoltà di Scienze M.F.N., Università di Roma, 100185, Roma, (Italy)

    Elisabetta Colletti, Laura Frontali & Claudio Palleschi

Authors
  1. Elisabetta Colletti
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  2. Laura Frontali
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  3. Claudio Palleschi
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  4. Micheline Wesolowski
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  5. Hiroshi Fukuhara
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Communicated by F. Kaudewitz

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Colletti, E., Frontali, L., Palleschi, C. et al. Two isoaccepting seryl tRNAs coded by separate mitochondrial genes in yeast. Molec. Gen. Genet. 175, 1–4 (1979). https://doi.org/10.1007/BF00267848

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  • DOI: https://doi.org/10.1007/BF00267848

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