Summary
Thirty somatic hybrids between Solanum tuberosum and Solanum brevidens were analysed for mitochondrial and chloroplast genome rearrangements. In all cases, the chloroplast genomes were inherited from one of the parental protoplast populations. No chloroplast DNA alterations were evident but a range of mitochondrial DNA alterations, from zero to extensive intra- and inter-molecular recombinations, were found. Such recombinations involved specific ‘recombination hot spots’ in the mitochondrial genome. Not all hybrids regenerated from a common callus possessed identical mitochondrial genomes, suggesting that sorting out of mitochondrial populations in the callus may have been incomplete at the plant regeneration stage. Sorting out of organelles in planta was not observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Austin S, Baer MA, Helgeson JP (1985) Transfer of resistance to potato leaf roll virus from Solarium brevidens into Solanum tuberosum by somatic fusion. Plant Sci 39:75–82
Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984) Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196:244–253
Barsby TL, Shepard JF (1983) Regeneration of plants from mesophyll protoplasts of Solanum species of the Etuberosa group. Plant Sci Lett 31:101–105
Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens. Plant Cell Rep 3:165–167
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Boeshore ML, Lifshitz I, Hanson MR, Izhar S (1983) Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants. Mol Gen Genet 190:459–467
Chetrit P, Mathieu C, Vedel F, Pelletier G, Primard C (1985) Mitochondrial DNA polymorphism induced by protoplast fusion in Cruciferae. Theor Appl Genet 69:361–366
Fluhr R, Aviv D, Edelman M, Galun E (1983) Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana. Theor Appl Genet 65:289–294
Fluhr R, Aviv D, Galun E, Edelman M (1984) Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombination types. Theor Appl Genet 67:491–497
Fluhr R, Edelman M (1981) Conservation of sequence arrangement among higher plant chloroplast DNAs: molecular cross hybridization among the Solanaceae and between Nicotiana and Spinacia. Nucleic Acids Res 9:6841–6853
Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D (1982) Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet 186:50–56
Gleba Y, Kolesnik NN, Meshkene IV, Cherep NN, Parokonny AS (1984) Transmission genetics of the somatic hybridization process in Nicotiana. 1. Hybrids and cybrids among the regenerates from cloned protoplast fusion products. Theor Appl Genet 69:121–128
Gleba Y, Komarnitsky IK, Kolesnik NN, Meshkene IV, Martyn GI (1985) Transmission genetics of the somatic hybridization process in Nicotiana. 2. Plastome heterozygotes. Mol Gen Genet 198:476–481
Glimelius K, Chen K, Bonnett HT (1981) Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid plants. Planta 153:504–510
Iwai S, Nakata K, Nagao T, Kawashima N, Matsuyama S (1981) Detection of the Nicotiana rustica chloroplast genome coding for the large subunit of Fraction I protein in a somatic hybrid in which only the TV. tabacum chloroplast genome appeared to have been expressed. Planta 152:478–480
Kemble RJ, Gunn RE, Flavell RB (1980) Classification of normal and male-sterile cytoplasms in maize. 2. Electrophoretic analysis of DNA species in mitochondria. Genetics 95:451–458
Kemble RJ, Shepard JF (1984) Cytoplasmic DNA variation in a potato protoclonal population. Theor Appl Genet 69:211–216
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12:9249–9261
Matthews BF, Widholm JM (1985) Organelle DNA compositions and isoenzyme expression in an interspecific somatic hybrid of Daucus. Mol Gen Genet 198:371–376
Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance. Chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris and isolation of their somatic hybrids. Mol Gen Genet 179:693–698
Nagy F, Lazar G, Menczel L, Maliga P (1983) A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA. Theor Appl Genet 66:203–207
Nagy F, Torok I, Maliga P (1981) Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Mol Gen Genet 183:437–439
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell 29:537–550
Rose RJ, Johnson LB, Kemble RJ (1986) Restriction endonuclease studies on the chloroplast and mitochondrial DNAs of alfalfa (Medicago saliva L.) protoclones. Plant Mol Biol 6:331–338
Rothenberg M, Boeshore ML, Hanson MR, Izhar S (1985) Intergenomic recombination of mitochondrial genomes in a somatic hybrid plant. Curr Genet 9:615–618
Shepard JF (1980) Mutant selection and plant regeneration from plant mesophyll protoplasts. In: Rubenstein I, Gengenbach B, Phillips RL, Green CE (eds) Emergent techniques for the genetic improvement of crops. University Minnesota Press, Minneapolis, pp 185–219
Sidorov VA, Menzcel L, Nagy F, Maliga P (1981) Chloroplast transfer in Nicotiana based on metabolic complementation between irradiated and iodoacetate treated protoplasts. Planta 152:341–345
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Kemble, R.J., Barsby, T.L., Wong, R.S.C. et al. Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens . Theoret. Appl. Genetics 72, 787–793 (1986). https://doi.org/10.1007/BF00266546
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00266546