Summary
This paper reports that the opaque-6 (o6) mutation of maize, which causes seedling lethality and interferes in the endosperm with the synthesis of zeins and b-32 protein, is a proline requiring mutant functionally allelic to proline-1 (pro-1). Furthermore, immunological studies on the b-32 content of ten independently originated o6 and pro-1 alleles demonstrated that four alleles contain an apparently normal b-32 protein while the others are either devoid of it or contain trace amounts of cross-reacting proteins of lower molecular weight.
Similar content being viewed by others
References
Bertani A, Tonelli C, Gavazzi G (1980) Determination of Δ′-pyrroline-5-carboxilic acid reductase in proline-requiring mutants of Zea mays L. Maydica 25:17–24
Dierks-Vending C, Tonelli C (1982) Metabolism of praline, glutamate, and ornithine in proline mutant root of Zea mays L. Plant Physiol 69:130–134
Di Fonzo N, Manzocchi L, Salamini F, Soave C (1986) Purification and properties of an endosperm protein of maize associated with the opaque-2 and opaque-6 genes. Planta 167:587–594
Gavazzi G, Nava-Racchi M, Tonelli C (1975) A mutation causing proline requirement in Zea mays. Theor Appl Genet 46:339–345
Gavazzi G, Todesco G, Neuffer MG (1978) Location of the pro mutant to chromosome arm. Maize Genet Coop Newslett 52:66–67
Gengenbach BG (1977) Development of maize caryopses resulting from in vitro pollination. Planta 134:91–93
Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T. Nature 227:680–684
Ma Y, Nelson OE (1975) Amino acid composition and storage proteins in two new high-lysine mutants of maize. Cereal Chem 52:412–419
Motto M, Marotta R, Di Fonzo N, Soave C, Salamini F (1986) Ds-induced alleles at the Opaque-2 locus of maize. Genetics 112:121–133
Nelson OE, Burr B (1973) Biochemical genetics of higher plants. Annu Rev Plant Physiol 24:493–518
Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346–356
Racchi ML, Gavazzi G, Monti D, Manitto P (1978) An analysis of the nutritional requirements of the pro mutants in Zea mays. Plant Sci Lett 13:357–364
Racchi ML, Gavazzi G, Dierks-Ventling C, King PJ (1981) Characterization of proline-requiring mutants in Zea mays L. Z Pflanzenphysiol 101:303–311
Shimamoto KO, Nelson OE (1981) Movement of 14C-compounds from material tissue into maize seeds grown in vitro. Plant Physiol 67:429–432
Soave C, Tardani L, Di Fonzo N, Salamini F (1981) Zein level in maize endosperm depends on a protein under the control of the opaque-2 and opaque-6 loci. Cell 27:403–410
Soave C, Salamini F (1984) Organization and regulation of zein genes in maize endosperms. Philos Trans R Soc Lond, Ser B 304:341–347
Tonelli C, Racchi ML, Gavazzi G, Beretta G (1982) In vitro expression of proline-requiring mutants in Zea mays L. Z Pflanzenphysiol 107:437–442
Tonelli C, Racchi ML, Viani I, Gavazzi G (1984) In vitro development of proline requiring mutant maize kernels. Atti Assoc Genet Ital 30:231
Author information
Authors and Affiliations
Additional information
Communicated by P. L. Pfahler
Rights and permissions
About this article
Cite this article
Manzocchi, L., Tonelli, C., Gavazzi, G. et al. Genetic relationship between o6 and pro-1 mutants in maize. Theoret. Appl. Genetics 72, 778–781 (1986). https://doi.org/10.1007/BF00266544
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00266544