Skip to main content
Log in

Genetic relationship between o6 and pro-1 mutants in maize

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

This paper reports that the opaque-6 (o6) mutation of maize, which causes seedling lethality and interferes in the endosperm with the synthesis of zeins and b-32 protein, is a proline requiring mutant functionally allelic to proline-1 (pro-1). Furthermore, immunological studies on the b-32 content of ten independently originated o6 and pro-1 alleles demonstrated that four alleles contain an apparently normal b-32 protein while the others are either devoid of it or contain trace amounts of cross-reacting proteins of lower molecular weight.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bertani A, Tonelli C, Gavazzi G (1980) Determination of Δ′-pyrroline-5-carboxilic acid reductase in proline-requiring mutants of Zea mays L. Maydica 25:17–24

    Google Scholar 

  • Dierks-Vending C, Tonelli C (1982) Metabolism of praline, glutamate, and ornithine in proline mutant root of Zea mays L. Plant Physiol 69:130–134

    Google Scholar 

  • Di Fonzo N, Manzocchi L, Salamini F, Soave C (1986) Purification and properties of an endosperm protein of maize associated with the opaque-2 and opaque-6 genes. Planta 167:587–594

    Google Scholar 

  • Gavazzi G, Nava-Racchi M, Tonelli C (1975) A mutation causing proline requirement in Zea mays. Theor Appl Genet 46:339–345

    Google Scholar 

  • Gavazzi G, Todesco G, Neuffer MG (1978) Location of the pro mutant to chromosome arm. Maize Genet Coop Newslett 52:66–67

    Google Scholar 

  • Gengenbach BG (1977) Development of maize caryopses resulting from in vitro pollination. Planta 134:91–93

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T. Nature 227:680–684

    Google Scholar 

  • Ma Y, Nelson OE (1975) Amino acid composition and storage proteins in two new high-lysine mutants of maize. Cereal Chem 52:412–419

    Google Scholar 

  • Motto M, Marotta R, Di Fonzo N, Soave C, Salamini F (1986) Ds-induced alleles at the Opaque-2 locus of maize. Genetics 112:121–133

    Google Scholar 

  • Nelson OE, Burr B (1973) Biochemical genetics of higher plants. Annu Rev Plant Physiol 24:493–518

    Google Scholar 

  • Peterson GL (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83:346–356

    Google Scholar 

  • Racchi ML, Gavazzi G, Monti D, Manitto P (1978) An analysis of the nutritional requirements of the pro mutants in Zea mays. Plant Sci Lett 13:357–364

    Google Scholar 

  • Racchi ML, Gavazzi G, Dierks-Ventling C, King PJ (1981) Characterization of proline-requiring mutants in Zea mays L. Z Pflanzenphysiol 101:303–311

    Google Scholar 

  • Shimamoto KO, Nelson OE (1981) Movement of 14C-compounds from material tissue into maize seeds grown in vitro. Plant Physiol 67:429–432

    Google Scholar 

  • Soave C, Tardani L, Di Fonzo N, Salamini F (1981) Zein level in maize endosperm depends on a protein under the control of the opaque-2 and opaque-6 loci. Cell 27:403–410

    Google Scholar 

  • Soave C, Salamini F (1984) Organization and regulation of zein genes in maize endosperms. Philos Trans R Soc Lond, Ser B 304:341–347

    Google Scholar 

  • Tonelli C, Racchi ML, Gavazzi G, Beretta G (1982) In vitro expression of proline-requiring mutants in Zea mays L. Z Pflanzenphysiol 107:437–442

    Google Scholar 

  • Tonelli C, Racchi ML, Viani I, Gavazzi G (1984) In vitro development of proline requiring mutant maize kernels. Atti Assoc Genet Ital 30:231

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by P. L. Pfahler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Manzocchi, L., Tonelli, C., Gavazzi, G. et al. Genetic relationship between o6 and pro-1 mutants in maize. Theoret. Appl. Genetics 72, 778–781 (1986). https://doi.org/10.1007/BF00266544

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00266544

Key words

Navigation