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Immobilization of Penicillium chrysogenum: spore growth on Celite

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Summary

Penicillium chrysogenum spores have been immobilized by adsorption on two grades of wet or dry diatomaceous earth particles, Chromosorb-W and Celite R-633. Almost 90% of the spores were adsorbed within 2 h and those remaining in suspension were removed by washing to minimise the growth of free mycelia. After germination the immobilized biomass was almost independent of the spore loading on the particles and whether or not the spore suspension was added to wet or dry particles. The free biomass obtained was less than 5% of the immobilized biomass.

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Author notes

  1. G. Lilley

    Present address: Celltech Ltd, 216 Bath Road, SL1 4EN, Slough, Berks, UK

Authors and Affiliations

  1. SERC Centre for Biochemical Engineering, Department of Chemical and Biochemical Engineering, University College London, Torrington Place, WC1E 7JE, London, UK

    T. Keshavarz, E. Walker, R. Eglin, G. Lilley & M. D. Lilly

  2. School of Biotechnology, Polytechnic of Central London, 115 New Cavendish Street, W1M 8JS, London, UK

    G. Holt

  3. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, UK

    A. T. Bull

Authors
  1. T. Keshavarz
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  2. E. Walker
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  3. R. Eglin
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  4. G. Lilley
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  5. G. Holt
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  6. A. T. Bull
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  7. M. D. Lilly
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Keshavarz, T., Walker, E., Eglin, R. et al. Immobilization of Penicillium chrysogenum: spore growth on Celite. Appl Microbiol Biotechnol 30, 487–491 (1989). https://doi.org/10.1007/BF00263853

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  • DOI: https://doi.org/10.1007/BF00263853

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