Summary
A Collagen/Vicryl (Polyglactin) composite membrane (developed for use in urinary tract surgery) has been incubated in cultures of radioactively labelled urinary tract pathogens vis Escherichia coli, Staphylococcus epidermidis, and Proteus mirabilis for up to 1h. For comparison, collagen film, Vicryl mesh, and a number of absorbable and nonabsorbable sutures were similarly tested. Following incubation, samples were also examined by scanning electron microscopy. Under the experimental conditions employed, only minimal adherence of the micro-organisms to the collagen coated Vicryl mesh, its two individual components, as well as Vicryl and nylon sutures was observed. Significantly greater numbers of bacteria, however, ahered to silk and Chromic Catgut. The results suggest that adherence of microorganisms to the prosthesis even in infected urine is unlikely to develop into a microcolony of bacteria. However, it should be emphasised that great care must be excercised when extrapolating from the in-vitro to the in-vivo situation.
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References
Böyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest [Suppl] 21:77–89
Gorham SD, Monsour MJ, Scott R (1987) The in-vitro assessment of a collagen/vicryl (polyglactin) composite film together with candidate suture materials for use in urinary tract surgery. I. Physical testing. Urol Res 15:53–59
Gorham SD, Anderson JD, Monsour MJ, Scott R (1988) The in-vitro assessment of a collagen/vicryl (polyglactin) composite film together with candidate suture materials for use in urinary tract surgery. II. Surface deposition of urinary salts. Urol Res 16:111–117
Holbrook MC (1982) The resistance of polyglycolic acid sutures to attack by infected human urine. Br J Urol 54:313–315
Holderbaum D, Hall GS, Ehrhart LA (1986) Collagen binding to Staphylococcus aureus. Infect Immun 54:359–364
Mohammed R, Monsour MJ, Gorham SD, French DA, Scott R (1987) The use of a biodegradable collagen/vicryl composite membrane to repair partial nephrectomy in rabbits. Urol Res 15:239–242
Monsour MJ, Mohammed R, Gorham SD, French DA, Scott R (1987) An assessment of a collagen/vicryl composite membrane to repair defects of the urinary bladder in rabbits. Urol Res 15:235–238
Scott R, Mohammed R, Gorham SD, French DA, Monsour MJ, Chivas A, Hyland T (in press) The evolution of a biodegradable membrane for use in urological surgery. A summary of 109 in-vivo experiments in the rabbit. Br J Urol
Sebeseri O, Keller U, Spreng P, Tscholl R, Zingg E (1985) The physical properties of polyglycolic acid suture (dexon) in sterile and infected urine. Invest Urol 12:490–493
Speziale P, Raucci G, Visai L, Switalski LM, Timpl R, Hook M (1986) Binding of collagen to Staphylococcus aureus Cowan I. J Bacteriol 167:77–81
Williams DF (1980) The effect of bacteria on absorbable sutures. J Biomed Mater Res 14:329–338
Yudofsky SC, Scott FB (1969) Urolithiasis on suture materials: its importance, pathogenesis and prophylaxis. An introduction to the monofilament teflon suture. J Urol 102:745–749
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Gemmell, C.G., Gorham, S.D., Monsour, M.J. et al. The in-vitro assessment of a Collagen/Vicryl (Polyglactin) composite film together with candidate suture materials for potential use in urinary tract surgery. Urol. Res. 16, 381–384 (1988). https://doi.org/10.1007/BF00256047
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DOI: https://doi.org/10.1007/BF00256047