Summary
In order to develop a more useful system for extracellular protein production from Escherichia coli, we have constructed the new excretion vectors pEAP82-1, pEAP82-2, and pEAP82-3. These vectors have, respectively, a single, double, and triple penicillinase promoter upstream of a penicillinase structural gene; E. coli HB101 carrying pEAP82-2 or pEAP82-3 produced respectively, about twice or three times as much penicillinase protein than that produced by E. coli carrying pEAP82-1, and 70% to 80% of the protein was excreted into the culture medium. The E. coli carrying pEAP82-3 was cultivated at various temperatures and it was observed that the optimum for extracellular penicillinase production was 30°–37°C. Using this multi-promoter excretion system, the amount of extracellular production of human growth hormone was increased by several fold as observed with penicillinase excretion.
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Murakami, Y., Furusato, T., Kato, C. et al. Construction of new excretion vectors: two and three tandemly located promoters are active for extracellular protein production from Escherichia coli . Appl Microbiol Biotechnol 30, 619–623 (1989). https://doi.org/10.1007/BF00255369
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DOI: https://doi.org/10.1007/BF00255369