Summary
Using conventional techniques interspecific hybridization between N. glutinosa (2n=24) and N. megalosiphon (2n=40) results in failure while the reciprocal is successful. Attempts were made here to obtain an F1 hybrid of N. glutinosa X N. megalosiphon by embryo rescue. These reciprocal hybrids resembled each other in their phenotypic and chromosomal behaviour, i.e. there is broad spectrum of phenotypic variation coupled to chromosomal range from 2n-28 to 32. This may be due to the functional disharmony between chromosome sets of two unrelated species resulting in the elimination of chromosomes.
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Subhashini, U., Venkateswarlu, T., Anjani, K. et al. In vitro hybridization in an incompatible cross Nicotiana glutinosa X Nicotiana megalosiphon . Theoret. Appl. Genetics 71, 545–549 (1985). https://doi.org/10.1007/BF00251203
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DOI: https://doi.org/10.1007/BF00251203