Summary
The aim of this study was to investigate the detoxicant Mixed Function Oxidase system in two species of Antarctic fish, C. hamatus and P. bernacchii, collected during the Antarctic summer of 1989–1990 at the Italian Scientific Station at Terra Nova Bay (Ross Sea). Several specimens were induced by injection of phenobarbital (PB), 3-methylcholanthrene (3-MC) and PCBs in the caudal vein. The results show significant differences between the two species. In C. hamatus, basal activity of benzo(a)pyrene monooxygenase (BPMO) was among the lowest measured even in fish of temperate seas, and in P. bernacchii it was 20 times lower. The values of regenerating activities (NADPH-cytochrome c reductase (NADPH-CYTCRED), NADH-cytochrome c reductase (NADH-CYTCRED) and NADH-ferricyanide reductase (NADH-FERRIRED) suggest that the two species use different electron donor molecules. Injection of chemicals in the caudal vein did not provoke induction of MFO activity in P. bernacchii. In C. hamatus, phenobarbital and PB-type inducers did not cause induction but there was a statistically significant response to 3-MC.
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References
Coon MJ, Persson AV (1980) Microsomal Cytochrome P-450: A Central Catalyst in Detoxication Reactions. In: Jakoby WB, Academy Press, Inc. U.S.A Enzymatic Basis of Detoxication, vol. 1, pp 117–130
Chambers JE and Yarbrough JD (1976) Xenobiotic biotransformation systems in fishes. Comp Biochem Physiol 55C:77–84
De Matteis F (1984) Metabolismo epatico di sostanze esogene, influenze sulla tossicita' e interazioni metaboliche. 47° Congresso della Societa' Italiana di Medicina del Lavoro e Igiene Industriale. Assisi 17–20 Ottobre 1984
Dewitt HH, Heemstra PC, Gon O (1990) Nototheniidae. In: Gon O, Heemstra PC (eds), Fishes of the Southern Ocean. JLB Smith Institute of Ichthyology, Grahamstown, pp 279–331
di Prisco G, D' Avino R, Camardella E, Caruso C, Romano M, Rutigliano B (1988) Pesci antartici e adattamento alle basse temperature. Acqua-Aria 4:445–451
Eisele TA, Coulombe RA, Pawlowski NE, Nixon JE (1984) The effects of route of exposure and combined exposure of mixed function oxidase inducers and suppressors on hepatic parameters in rainbow trout (Salmo gairdneri). Aquatic Toxicol 5:211–226
Elskus AA, Stegeman JJ, Susani LC, Black D, Pruell RJ, Fluck SJ (1989) Polychlorinated biphenyls concentration and cytochrome P-450E expression in Winter Flounder from contaminated environments. Mar Environ Res 28:25–30
Fent K, Stegeman JJ (1991) Effects of tributyltin chloride in vitro on the hepatic microsomal monooxygenase system in the fish Stenotumus chrysops. Aquatic Toxicol 20:159–168
Focardi S, Fossi C, Leonzio C, Di Simplicio P (1989) Mixed Function Oxidase activity and conjugating enzymes in two species of Antarctic fish. Mar Environ Res 28:31–33
Focardi S, Lari L, Marsili L (1992) PCB congeners, DDTs and hexachlorobenzene in Antarctic fish from Terranova Bay (Ross Sea). Antarctic Sci 4:151–154
Fossi C, Leonzio C, Focardi S (1989) Regulatory detoxication responses in gobiidae experimentally exposed to PCBs. Ecotoxicol Environ Safety 18:11–14
James MO, Bend JR (1980) Polycyclic Aromatic Hydrocarbon Induction of Cytochrome P-450-Dependent Mixed-Function Oxidases in Marine Fish. Toxicol Appl Pharmacol 54:117–133
James MO, Khan MAQ, Bend JR (1979) Hepatic Microsomal Mixed-Function Oxidase activities in several marine species common to coastal Florida. Comp Biochem Physiol 62C: 155–164
Kappas A, Alvares AP (1975) How the liver metabolizes foreign substances. Sci Am 232:22–31
Koivusaari U, Pesonen M, Hanninen O (1984) Polysubstrate monooxygenase activity and sex hormones in pre-and postspawning rainbow trout (Salmo gairdneri). Aquatic Toxicol 5:67–76
Kurelec ie725–01, Britvic S, Rijavec M, Muller WEG, Zahn RK (1977) Benzo(a)pyrene monooxygenase induction in marine fish-Molecular response to oil pollution. Mar Biol 44:211–216
Lindstrom-Seppa P (1985) Seasonal variation of the xenobiotic metabolizing enzyme activities in the liver of male and female vendace (Coregonus albula L). Aquatic Toxicol 6:323–331
Livingstone DR, Farrar S (1984) Tissue and subcellular distribution of enzyme activity of mixed-function and benzo(a)pyrene metabolism in the common mussel Mytilus edulis. Sci Total Environ 39:209–235
Melancon MJ, Yeo SE, Lech JJ (1987) Induction of hepatic microsomal monooxygenase activity in fish by exposure to river water. Environ Toxicol Chem 6:127–135
Murphy PG (1972) Sulfuric acid for the cleanup of animal tissues for analyses of acid-stable chlorinated hydrocarbon residues. AOAC 55(6):1360–1362
Payne JF (1984) Mixed-function oxigenases in biological monitoring programs: Review of potential usage in different phyla of aquatic animals. In: Ecotoxicological Testing for the Marine Environment, vol 1:625–655
Payne JF, Maloney RA, Rahimtula (1979) Are petroleum hydrocarbons an important source of mutagens in the marine environment? Proceedings of the 1979 Oil Spill Conference. American Petroleum Institute. Publication No. 4308. Washington, D.C pp 533–536
Rijorec M, Britvic' S, Protic' M, Kurelec B (1981) Detection of the presence of xenobiotics in sea water samples from the Rijeka Bay applying benzo(a)pyrene monooxygenase induction. Thalassia Jugoslavica 17:245–260
Stegeman JJ (1989) Cytochrome P-450 forms in fish: catalytic, immunological and sequence similarities. Xenobiotica 19(10):1093–1110
Stegeman JJ, Chevion M (1980) Sex differences in P-450 and Mixed Function Oxidase activity in gonadally mature trout. Biochem Pharmacol 29:553–558
Winston GW, Shane BS, Henry CB (1988) Hepatic monooxygenase Induction and Promutagen Activation in Channel Catfish from a Contaminated River Basin. Ecotoxicol Environ Safety 16:258–271
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Focardi, S., Fossi, C., Lari, L. et al. Induction of mixed function oxidase (MFO) system in two species of antarctic fish from Terra Nova Bay (Ross Sea). Polar Biol 12, 721–725 (1992). https://doi.org/10.1007/BF00238873
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DOI: https://doi.org/10.1007/BF00238873